What is the difference between NB100-105 and NB100-123?

NB100-105 and NB100-123 are similar as far as immunogens and clones are concerned. However, they are different with respect to their purification process, concentrations and recommended dilutions. NB100-123 is Protein A purified IgG2b with a concentration of 1.5 mg/ml, whereas NB100-105 is Protein G purified IgG2b with 4.0 mg/ml concentration. Another difference is the recommended dilutions (because of the differences in their concentration).

NB100-105 has the same clone name (H1alpha67) as NB100-123.Are these the same clone?

These are the same clones, however their purifications are slightly different (and thus their recommended dilutions for certain applications are a bit different, please see the section "Applications/Dilutions" on the specific product pages for differences).NB100-123: Protein A purifiedThe basis for purification of IgG, IgG fragments and IgG subclasses is the high affinity of protein A and protein G for the Fc region of polyclonal and monoclonal IgG-type antibodies. Protein A and protein G are bacterial proteins, which, when coupled to chromatography matrix such as Agarose or Sepharose, generate exceptionally useful, easy to use media (resin) for many routine applications. Protein A/G is a recombinant of Protein A and Protein G that has the additive binding properties of both proteins. Protein A, protein G and protein A/G can be used for purification of monoclonal IgG-type antibodies, purification of polyclonal IgG subclasses, and the adsorption and purification of immune complexes involving IgG. IgG subclasses can be isolated from cell culture supernatants and serum and from ascites fluid.Lastly, we have been producing NB100-105 for a longer time period, and thus why it has considerably more publication references than NB100-123.

Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?

HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.

Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.

All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, we suggest the following four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products have been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449) (cited in at least 31 peer reviewed publications).

I would like to know, does a path exist for detection of HIF 1 in venous blood before and after revascularization of the leg?

We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present, but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.

What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?

The theoretical molecular weight of HIF 1-alpha is ~93kDa. However, you will likely see a band between 100-120kDa due to phosphorylation.

We got the Hif1a (NB100-105) antibody from you guys. I used the concentration that is mentioned on your website, but I am getting a band of a completely different size (~70kDa) and not the 120 kDa mentioned.

HIF-1 alpha is a notoriously difficult protein to work with due to its rapid degradation. Therefore, the ~70kDa bands are most likely degradation products. It is very important to lyse the cells in hypoxic conditions. We strongly recommend lysing the cells directly into the Laemmli buffer and doing that quickly, so that the exposure to oxygen is minimized.Please go through our hypoxia related FAQs, you should find them very informative.Also, running a positive control may help confirm the band specificity in your samples. You may prepare them yourself or choose some from our catalog, for example: 1) HeLa Hypoxic / Normoxic Cell Lysate (NBP2-36452)2) HeLa Hypoxic (CoCl2) / Normoxic Cell Lysate (NBP2-36450)

I performed several Western Blots of HIF-1 alpha with different lysis buffers, whole lysates, and cytoplasm/nuclei extractions. I can’t seem to get a good western blot (poor signal, band much lower than expected, etc.). Can someone suggest some technical considerations/tricks I should consider using?

A major issue that researchers working with HIF-1 alpha is degradation due to exposure to oxygen. In western blot, this results in a weaker band and/or the appearance of multiple low molecular weight bands (40-80 kDa). We recommend preparing the lysates after collection of cells/tissues as quickly as possible (on ice), preferably in a hypoxic chamber. We also recommend including a true hypoxia mimetic control (eg: cells treated with CoCl2, DMOG… etc.). The controls help distinguish your band of interest from potential degradation/dimer bands.For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.

I am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research. Can someone let me know if I’m getting the right HIF band or just some non-specific bands? Thanks.

(1) HIF-1 alpha’s theoretical molecular weight is 93kDa. The post translationally modified/ubiquitinated form of HIF-1 alpha protein (fails to undergo proteasomal degradation) shows up as a band in the 110-130 kDa range on a Western blot.(2) The dimeric protein may appear at a position above 200 kDa on non-reducing gels.(3) Importantly, HIFs are among the most rapidly degradable proteins; therefore, sample preparation is highly important when analyzing HIF1 alpha or HIF2 alpha. When degraded, HIF-1 alpha may show up between 40-80 kDa position on Western blot. Degradation may be avoided by preparing the samples as soon as possible after collection of cells/tissues in hypoxic chamber. Notably, the tissues/cells should be kept on ice during lysate preparation and the lysates should be analyzed as soon as possible.(4) For troubleshooting suggestions/feedback on more than 25 similar frequently asked questions, I would recommend visiting Novus page: FAQs - Hypoxia and HIFs (5) Last but not the least, Novus technical support team may be contacted via email

I have Hif1a nuclear protein extract at -80C. I am wondering if anyone knows how long it would be good for at that temperature since HIf1a is known to be degraded easily.Thank you!

You could try a few things to further inhibit the degradation.1) Use the protease inhibitors (if you are not already using them).2) Lyse cells into a buffer that contains SDS or LDS (eg: Laemmli's buffer), since SDS and LDS denature and inhibit proteases. Lysis may even be performed with reducing agents in the buffer (eg. DTT), but this will make your lysates unsuitable for BCA assay.3) Lysing samples rapidly ensures that the samples are instantly homogenized (it also shears DNA released by the SDS).5) Flash-freezing samples in liquid nitrogen rather than freezing at -80*C reduces the window of time for protease activity.6) Freeze samples in individual aliquots, instead of thawing the same vial multiple times.

I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?

CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.

I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?

CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.

Is cross-reactivity with HIF-2 alpha tested/predicted?

Although we don’t have cross-reactivity data with regards to HIF-2 alpha, we predict minimal cross-reactivity based on low sequence similarity observed from BLAST analysis between HIF-1 alpha and HIF-2 alpha.

We ordered and received the HIF-1 alpha antibody NB100-449 and on the packing slip it says that is prepared in TBS+0.1% BSA. I will be using it for western blots. DO you think my choice of blocking buffer (milk or Blotto) could interfere with the activity of this antibody or should it be necessarily BSA based?

Choice of blocking buffer is entirely within your discretion; it will not affect the antibody binding activity. Please note, some blocking buffers may work better than others and sometimes optimization is needed.When working with hypoxia there are other important factors to consider, as HIF-alpha is very easily degraded. The lysates should be freshly prepared. Also lysate preparation should be as quick as possible to avoid any exposure to oxygen - we recommend lysing cells directly into the SDS sample loading buffer (Laemmli buffer). We also highly recommend using positive control (you can prepare them yourself or choose some of those we have for sale).I have attached some additional information that you may find quite useful. Also here are some hypoxia related FAQ addressing common concerns.

Our lab recently ordered NB100-449, HIF-1 alpha antibody. Unfortunately an inexperienced technician stored it at -20C rather than 4C for approximately 2 days. Have you done any tests to determine antibody functionality if frozen?

The recommended storage condition of HIF-1 alpha antibody NB100-449 is 4C and we highly recommended not storing the product lower than the freezing point, as it may potentially disrupt the protein folding and destroy the antigen binding site of the antibody. Since we likely have not tested a storage condition of -20C for this antibody, we cannot really say if this antibody has been impaired by the storage condition. Our recommendation would be to test the antibody in a small portion of your treated cell line and see if the antibody is still reactive to the HIF-1 alpha protein.

Could you clarify if Yeast is a reactive species for HIF1a product #NB100-105?

This HIF-1 alpha antibody has not been tested in yeast. The homology is not significantly homologous so we do not believe there will be cross reactivity to the yeast protein.

Can HIF-1 alpha Antibody (Dylight 488), product NB100-479G, react with goat species? Does this product have preservatives in it?

NB100-479G has not been tested in goat species. Only the listed species on the product page and datasheet will be guaranteed. Reactivity: Hu, Mu, Rt, Ca, Fi, Ha, Pm, RbThe immunogen for this antibody corresponds to amino acids 530-825 of mouse Hifa. Running a sequence alignment of this sequence with the goat sequence found on UniProt yields around 81% homology.Mouse Hif1a: https://www.uniprot.org/uniprot/Q61221#sequencesGoat Hif1a: https://www.uniprot.org/uniprot/A0A023R978#sequencesLastly, there is 0.05% Sodium Azide present in the formulation of the product. This is also listed on the product page and datasheet. Buffer: 50mM Sodium BoratePreservative: 0.05% Sodium Azide

What's the difference between NBP2-75977 vs NBP2-75978?

While the same immunogen was used to make both HIF-1 alpha antibodies, they are different clones, meaning they recognize a different epitope on the immunogen .

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HIF-1 alpha Antibody (H1alpha67)

Name: HIF-1 alpha Antibody (H1alpha67)
Brand: Novus Biologicals
SKU: NB100-123
Rating: 4.2 (9 reviews)

(161 Publications)

(9 Reviews)

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Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Results of in situ hybridization and immunohistochemistry on thin adjacent section to detect expression of HIF-1a1.2 mRNA and HIF1a protein in malignant and benign prostate tissue. In situ hybridization (antisense probe, Fig. 3a) and immunostaining with HIF1a Ab2 (Fig. 3c) on thin adjacent sections of NE-differentiated prostate adenocarcinoma showed co-localization of HIF1a1.2 transcript and HIF-1a protein. Incubation with sense probe did not generate any detectable hybridization signals (Fig. 3b). Both In situ hybridization (Fig. 3d antisense) and HIF-1a Ab2 immunostaining (Fig. 3f) were negative in non-NE-differentiated prostate adenocarcinoma. In situ hybridization with sense probe performed on non-NE-differentiated prostate cancer (Fig. 3e). Image collected and cropped by CiteAb from the following publication (//www.biomedcentral.com/1471-2407/10/385), licensed under a CC-BY license.

Orthogonal Strategies: Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Results of in situ hybridization and immunohistochemistry on thin adjacent section to detect expression of HIF-1a1.2 ...read more

Genetic Strategies: Knockdown Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - HIF-1 inhibits the apoptosis of hypoxic glioblastoma cells. U87MG cells were transfected with siRNA against HIF-1alpha. ...read more

Biological Strategies: Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - MLN4924 induces accumulation of HIF1a in a time dependent manner. Cells were treated with 0.1uM MLN4924 for indicated time ...read more

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis of HIF-1 alpha in human lung tissue. Image courtesy of product review by Aneta Gandjeva.

Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - MG132 attenuates the inhibitory effect of interleukin-1beta on HIF-1/AM axis. U87MG cells were incubated for 2 h in hypoxia (1% O2) with or without ...read more

Biological Strategies: Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear ...read more

Biological Strategies: Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). ...read more

Biological Strategies: Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Dapagliflozin protects against metabolic switch by inhibition of HIF-1 alpha. Representative images ...read more

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistochemical detection of HIF-1alpha protein in human tissues. The nature of the tissue is indicated on top of each figure. Original ...read more

Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining in pig endothelial cells under hypoxia condition using NB100-123. Image from verified customer review.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining on pig tissue (brown) using NB100-123. Image from verified customer review.

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining of biotin conjugated HIF-1 alpha (NB100-123B) in human kidney.

Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunoprecipitation of HIF-1alpha using NB100-123 in COS7 CoCl2 treated lysate. Heavy and light chains are also detected. Image using the HRP form of ...read more

Immunohistological and western blot analyses of HIF-1 alpha .(A) PAB liver stained by HIF-1 alpha (original magnification, ×20). (B) Sham-operated control liver stained by HIF-1 alpha (original magnification, ×20). ...read more

Biological Strategies: Immunocytochemistry/ Immunofluorescence: Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Images of HIF-1 alpha localization (red) in CTR and CoCl2-treated oAECs after 24 h ...read more

Western Blot: Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Image of HIF-1 alpha activation in AECs control cells (CTR) and after 24h of 10 and 50µM of CoCl2-treatment; HIF-1 alpha time course at ...read more

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistochemical detection of HIF-1 alpha protein in human tissues. The nature of the tissue is indicated on top of each figure. Original ...read more

Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistological & western blot analyses of HIF-1 alpha .(A) PAB liver stained by HIF-1 alpha (original magnification, ×20). (B) Sham-operated control ...read more

Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis of CBF treatment in HCT116 implanted mouse models & tumour tissue samples. (a) Tumour size versus CBF treatment. The ...read more

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - CycT alleviates tumor hypoxia. The effects of CycT on the levels of exogenous hypoxia-marker pimonidazole labeling (A), the levels of ...read more

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Schematic demonstration of the three HIF1 alpha antibodies used in this study & their epitopes (Figs. 2a & b). Ab1 is a polyclonal antibody raised ...read more

Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - GAD65/67-positive neurons expressed HIF-1 alpha under hypoxic conditions. A) HIF-1 alpha expression co-localized with GAD65/67-ir ...read more

Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Levels of GSH in cortical neurons exposed to hypoxia. A) During hypoxia, GSH increased in MAP2-ir neurons with round somata (bottom ...read more

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Results of in situ hybridization & immunohistochemistry on thin adjacent section to detect expression of HIF-1 alpha 1.2 mRNA & HIF1 alpha protein in ...read more

Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - HX-induced Sirt1 expression in white matter OPCs requires HIF1 alpha .(a) HIF1 alpha stabilization of Sirt1 transcript expression in OPCs as revealed by ...read more

Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - HIF-1 alpha expression in primary cortical neurons exposed to hypoxia/ischemia. A & A’) Neurons were double-stained for HIF-1 ...read more

Product Details

Summary

Reactivity	Hu, Mu, Rt, Po, Av, Bv, Ca, Ma, Pm, Rb, ShSpecies Glossary
Applications	WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IP, WB, ChIP, KD, KO
Clone	H1alpha67
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	1.0 mg/ml

Validated by:

Biological Strategies

Genetic Strategies

Orthogonal Strategies

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB100-123AF350

Alexa Fluor 405 NB100-123AF405

Alexa Fluor 488 NB100-123AF488

Alexa Fluor 532 NB100-123AF532

Alexa Fluor 594 NB100-123AF594

Alexa Fluor 647 NB100-123AF647

Alexa Fluor 700 NB100-123AF700

Alexa Fluor 750 NB100-123AF750

Allophycocyanin NB100-123APC

Biotin NB100-123B

CoraFluor 1 NB100-123CL1

DyLight 350 NB100-123UV

DyLight 405 NB100-123V

DyLight 488 NB100-123G

DyLight 550 NB100-123R

DyLight 594 NB100-123DL594

DyLight 650 NB100-123C

DyLight 680 NB100-123FR

DyLight 755 NB100-123IR

FITC NB100-123F

HRP NB100-123H

Janelia Fluor 525 NB100-123JF525

Janelia Fluor 585 NB100-123JF585

Janelia Fluor 635 NB100-123JF635

Janelia Fluor 669 NB100-123JF669

PE NB100-123PE

PerCP NB100-123PCP

mFluor Violet 450 SE NB100-123MFV450

mFluor Violet 500 SE NB100-123MFV500

mFluor Violet 610 SE NB100-123MFV610

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Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-123AF350
Alexa Fluor 405	NB100-123AF405
Alexa Fluor 488	NB100-123AF488
Alexa Fluor 532	NB100-123AF532
Alexa Fluor 594	NB100-123AF594
Alexa Fluor 647	NB100-123AF647
Alexa Fluor 700	NB100-123AF700
Alexa Fluor 750	NB100-123AF750
Allophycocyanin	NB100-123APC
Biotin	NB100-123B
CoraFluor 1	NB100-123CL1
DyLight 350	NB100-123UV
DyLight 405	NB100-123V
DyLight 488	NB100-123G
DyLight 550	NB100-123R
DyLight 594	NB100-123DL594
DyLight 650	NB100-123C
DyLight 680	NB100-123FR
DyLight 755	NB100-123IR
FITC	NB100-123F
HRP	NB100-123H
Janelia Fluor 525	NB100-123JF525
Janelia Fluor 585	NB100-123JF585
Janelia Fluor 635	NB100-123JF635
Janelia Fluor 669	NB100-123JF669
PE	NB100-123PE
PerCP	NB100-123PCP
mFluor Violet 450 SE	NB100-123MFV450
mFluor Violet 500 SE	NB100-123MFV500
mFluor Violet 610 SE	NB100-123MFV610

HIF-1 alpha Antibody (H1alpha67) Summary

Immunogen	This HIF-1 alpha Antibody (H1alpha67) was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].
Localization	HIF-1 alpha is a nuclear protein that activates gene transcription in response to reduced cellular O2 concentration.
Isotype	IgG2b
Clonality	Monoclonal
Host	Mouse
Gene	HIF1A
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Chromatin Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature
ELISA 1:100 - 1:2000. Use reported in scientific literature
Flow Cytometry 1:10 - 1:1000
Gel Super Shift Assays 1:1 - 1:100. Use reported in scientific literature
Immunoblotting reported in multiple pieces of scientific literature
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry 1:100 - 1:300
Immunohistochemistry-Paraffin 1:100 - 1:300
Immunoprecipitation 1:10
Knockdown Validated
Knockout Validated reported in scientific literature (PMID 27991597)
Western Blot 1:500 - 1:1000

Application Notes

By WB, this antibody recognizes bands at 120kDa representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 120kDa representing post-translational modifications. Nuclear extracts are recommended for WB.

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Control

Human Lung Whole Tissue Lysate (Adult Whole Normal)
COS-7 Nuclear Hypoxic Induced Cell Lysate
HeLa CoCl2 treated/Untreated Cell Lysate
HepG2 CoCl2 treated/Untreated Cell Lysate
HeLa Hypoxic / Normoxic Cell Lysate
HepG2 Hypoxic / Normoxic Cell Lysate
HIF-1 alpha Knockout CoCl2-treated/untreated HeLa Cell Lysate
HIF-1 alpha Knockout Hypoxic-treated/untreated HeLa Cell Lysate
NIH 3T3 CoCl2 treated/Untreated Cell Lysate
L929 CoCl2 treated/Untreated Cell Lysate
Raw 264.7 CoCl2 treated/Untreated Cell Lysate
PC12 CoCl2 treated/Untreated Cell Lysate
PC12 Hypoxic / Normoxic Cell Lysate
Raw 264.7 Hypoxic / Normoxic Cell Lysate
L929 Hypoxic / Normoxic Cell Lysate
NIH 3T3 Hypoxic / Normoxic Cell Lysate

Reviewed Applications

Read 9 Reviews rated 4.2

using
NB100-123 in the following applications:

Western Blot
Immunoprecipitation
Immunohistochemistry
Immunofluorescence
Immunohistochemistry-Paraffin
Immunocytochemistry

Publications

Read Publications using
NB100-123 in the following applications:

Chemotaxis
3 publications
ELISA
4 publications
FLOW
1 publication
GS
3 publications
ICC/IF
19 publications
IF/IHC
35 publications
IHC-P
29 publications
IP
3 publications
Immunohistochemistry
2 publications
Immunohistochemistry-Paraffin
1 publication
KO
1 publication
WB
57 publications
Western Blot
1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Rabbit reactivity reported in scientific literature (PMID: 16738327, 26339038).

Packaging, Storage & Formulations

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS with 1% BSA
Preservative	0.05% Sodium Azide
Concentration	1.0 mg/ml
Purity	Protein A purified

Alternate Names for HIF-1 alpha Antibody (H1alpha67)

AINT
anti-HIF-1 alpha
anti-HIF1A
ARNT interacting protein
ARNT-interacting protein
Basic-helix-loop-helix-PAS protein MOP1
BHLHE78
Class E basic helix-loop-helix protein 78
H1alpha67
HIF 1A
HIF1 alpha
HIF-1 alpha
HIF1
HIF1A
HIF-1a
HIF-1alpha
HIF-1-alpha
HIF1-alpha
hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
hypoxia-inducible factor 1-alpha
Member of PAS protein 1
member of PAS superfamily 1
MOP1
PAS domain-containing protein 8
PASD8

Background

Hypoxia contributes to the pathophysiology of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease (1). In cancer and particularly solid tumors, hypoxia plays a critical role in the regulation of genes involved in stem cell renewal, epithelial to mesenchymal transition (EMT), metastasis and angiogenesis. In the tumor microenvironment (TME), hypoxia influences the properties and function of stromal cells (e.g., fibroblasts, endothelial and immune cells) and is a strong determinant of tumor progression (2,3).

HIF-1 or hypoxia inducible factor 1 (predicted molecular weight 93kDa), is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. In its active form under hypoxic conditions, HIF-1 is stabilized by the formation of a heterodimer of HIF-1 alpha and ARNT/HIF-1 beta subunits. Nuclear HIF-1 engages p300/CBP for binding to hypoxic response elements (HREs). This process induces transcription and regulation of genes including EPO, VEGF, iNOS2, ANGPT1 and OCT4 (4,5).

Under normoxic conditions, the HIF-1 alpha subunit is rapidly targeted and degraded by the ubiquitin proteasome system. This process is mediated by prolyl hydroxylase domain enzymes (PHDs), which catalyze the hydroxylation of key proline residues (Pro-402 and Pro-564) within the oxygen-dependent degradation domain of HIF-1 alpha. Once hydroxylated, HIF-1 alpha binds the von Hippel-Lindau tumor suppressor protein (pVHL) for subsequent ubiquitination and proteasomal degradation (4). pVHL dependent regulation of HIF-1 alpha plays a role in normal physiology and disease states. Regulation of HIF-1 alpha by pVHL is critical for the suppressive function of FoxP3+ regulatory Tcells (6). Repression of pVHL expression in chronic lymphocytic leukemia (CLL) B cells leads to HIF-1 alpha stabilization and increased VEGF secretion (7).

References

1. Semenza, G. L., Agani, F., Feldser, D., Iyer, N., Kotch, L., Laughner, E., & Yu, A. (2000). Hypoxia, HIF-1, and the pathophysiology of common human diseases. Advances in Experimental Medicine and Biology.

2. Muz, B., de la Puente, P., Azab, F., & Azab, A. K. (2015). The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia. https://doi.org/10.2147/hp.s93413

3. Huang, Y., Lin, D., & Taniguchi, C. M. (2017). Hypoxia inducible factor (HIF) in the tumor microenvironment: friend or foe? Science China Life Sciences. https://doi.org/10.1007/s11427-017-9178-y

4. Koyasu, S., Kobayashi, M., Goto, Y., Hiraoka, M., & Harada, H. (2018). Regulatory mechanisms of hypoxia-inducible factor 1 activity: Two decades of knowledge. Cancer Science. https://doi.org/10.1111/cas.13483

5. Dengler, V. L., Galbraith, M. D., & Espinosa, J. M. (2014). Transcriptional regulation by hypoxia inducible factors. Critical Reviews in Biochemistry and Molecular Biology. https://doi.org/10.3109/10409238.2013.838205

6. Lee, J. H., Elly, C., Park, Y., & Liu, Y. C. (2015). E3Ubiquitin Ligase VHL Regulates Hypoxia-Inducible Factor-1 alpha to Maintain Regulatory T Cell Stability and Suppressive Capacity. Immunity. https://doi.org/10.1016/j.immuni.2015.05.016

7. Ghosh, A. K., Shanafelt, T. D., Cimmino, A., Taccioli, C., Volinia, S., Liu, C. G., ... Kay, N. E. (2009). Aberrant regulation of pVHL levels by microRNA promotes the HIF/VEGF axis in CLL B cells. Blood. https://doi.org/10.1182/blood-2008-10-185686

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Antibody (H1beta234) ...

NB100-124

Immunocytochemistry/ Immunofluorescence ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free

(4 Reviews)

(106 Publications)

Species: Bv, Ma, Hu, Mu, Pm, Rt, Sh

Applications: ChIP, CHIP-SEQ, GS, IB, ICC/IF, IHC, IHC-P, IP, WB

Erythropoietin/EPO
[HRP]

MEP00B

(105 Publications)

Species: Mu

Applications: ELISA

Von Hippel Lindau
Antibody (OTI1E1)

NBP2-45411

Species: Hu, Mu, Rt

Applications: IHC, IHC-P, WB

HIF-2 alpha/EPAS1
Antibody - BSA Free

NB100-122

Western Blot HIF-2 alpha/EPAS1 Antibody - BSA Free

(37 Reviews)

(823 Publications)

Species: Fi, Ha, Hu, Mu, Pm, Rb, Rt, Re, Sh

Applications: ChIP, Dual ISH-IHC, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, KO, PAGE, Simple Western, WB

AKT [p Ser473]
Antibody [Unconjugate ...

AF887

Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific

Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific

(49 Publications)

Species: Hu, Mu, Rt

Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB

Glut1 Antibody -
BSA Free

NB110-39113

Flow Cytometry Glut1 Antibody - BSA Free

(7 Reviews)

(62 Publications)

Species: Hu, Mu, Rb, Rt

Applications: ChIP, ChIP, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, Simple Western, WB

Carbonic Anhydrase
IX/CA9 Antibody - BSA ...

NB100-417

Immunoblotting Carbonic Anhydrase IX/CA9 Antibody - BSA Free

(7 Reviews)

(279 Publications)

Species: Hu, Mu, Pl, Rt

Applications: ChIP, Dual ISH-IHC, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr, PLA, Simple Western, WB

p53 Antibody (PAb
240) - BSA Free

NB200-103

Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free

(3 Reviews)

(42 Publications)

Species: Hu, Mu, Rt, Xp(-), Ye

Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB

ERK1 Antibody
(1E5) - BSA Free

NBP2-22203

Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free

Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free

(1 Publication)

Species: Hu, Pm, Mu, Rt

Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB

ERK2 Antibody
[Unconjugated]

AF1230

Immunohistochemistry ERK2 Antibody [Unconjugated]

Knockout Validated ERK2 Antibody [Unconjugated]

(3 Publications)

Species: Hu, Mu, Rt

Applications: IHC, KO, WB

TOR/mTOR [p
Ser2448] Antibody

NB600-607

Western Blot TOR/mTOR [p Ser2448] Antibody

(2 Reviews)

(9 Publications)

Species: Hu, Rt

Applications: ELISA, IHC, IHC-P, WB

HIF-1 alpha
Antibody (H1alpha67)

NB100-105

Flow Cytometry HIF-1 alpha Antibody (H1alpha67)

(45 Reviews)

(1040 Publications)

Species: Bv, Ca, Fe, Ma, Hu, Pm, Mu, Po, Pm, Rb, Rt, Sh, Xp

Applications: ChIP, ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, KO, LA, PLA, Simple Western, TCS, WB

iNOS Antibody -
BSA Free

NB300-605

(3 Reviews)

(130 Publications)

Species: Hu, Mu, Po, Rt

Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB

TIMP-1 [HRP]

DTM100

(97 Publications)

Species: Hu

Applications: ELISA

TNF-alpha
[Unconjugated]

210-TA

(3 Reviews)

(809 Publications)

Species: Hu

Applications: BA

EGLN1/PHD2 Antibody

NB100-137

Immunohistochemistry EGLN1/PHD2 Antibody

(12 Reviews)

(92 Publications)

Species: Hu, Mu, Pm, Rt

Applications: Flow, ICC/IF, IHC, IHC-P, IP, KD, KO, Simple Western, WB

Publications for HIF-1 alpha Antibody (NB100-123)(161)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Porcine, Primate, Rabbit, Sheep.

We have publications tested in 13 applications: Chemotaxis, ELISA, FLOW, GS, ICC/IF, IF/IHC, IHC-P, IP, Immunohistochemistry, Immunohistochemistry-Paraffin, KO, WB, Western Blot.

Showing Publications 1 - 10 of 161. Show All 161 Publications.

Publications using NB100-123	Applications	Species
Jenna Kerry, Erin J Specker, Morgan Mizzoni, Andrea Brumwell, Leslie Fell, Jenna Goodbrand, Michael N Rosen, James Uniacke Autophagy-dependent alternative splicing of ribosomal protein S24 produces a more stable isoform that aids in hypoxic cell survival. FEBS letters 2024-03-12 [PMID: 38281767]
Heyer, V;Reina-San-Martin, B; Optimal AID expression and efficient immunoglobulin class switch recombination are dependent on the Hypoxia-Inducible Factor European journal of immunology 2023-05-04 [PMID: 37143384] (WB, Mouse)	WB	Mouse
Li X, Ma TK, Wang M et al. YY1-induced upregulation of LncRNA-ARAP1-AS2 and ARAP1 promotes diabetic kidney fibrosis via aberrant glycolysis associated with EGFR/PKM2/HIF-1? pathway Frontiers in Pharmacology 2023-02-15 [PMID: 36874012]
Zheng Z, Xu T, Liu Z et al. Cryptolepine suppresses breast adenocarcinoma via inhibition of HIF-1 mediated glycolysis Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022-06-23 [PMID: 35753261] (WB, Human)	WB	Human
Wang N, Prabhakar NR, Nanduri J PROTEIN PHOSPHATASE 1 REGULATES REACTIVE OXYGEN SPECIES DEPENDENT DEGRADATION OF HISTONE DEACETYLASE 5 BY INTERMITTENT HYPOXIA American journal of physiology. Cell physiology 2022-06-15 [PMID: 35704695] (WB)	WB
Di Mattia M, Mauro A, Delle Monache S et al. Hypoxia-Mimetic CoCl2 Agent Enhances Pro-Angiogenic Activities in Ovine Amniotic Epithelial Cells-Derived Conditioned Medium Cells 2022-01-28 [PMID: 35159271] (WB, IF/IHC, Sheep)	WB, IF/IHC	Sheep
Medeiros PJ, Pascetta SA, Kirsh SM et al. Expression of hypoxia inducible factor-dependent Neuropeptide Y Receptors Y1 and Y5 sensitizes hypoxic cells to NPY stimulation The Journal of biological chemistry 2022-01-27 [PMID: 35093384] (WB, Human)	WB	Human
Binyamin O, Frid K, Keller G Et al. Comparing anti-aging hallmark activities of Metformin and Nano-PSO in a mouse model of genetic Creutzfeldt-Jakob Disease Neurobiology of Aging 2021-11-01 [PMID: 34875507] (IHC-P, Mouse)	IHC-P	Mouse
Kang HJ, Min BK, Choi WI et al. Pyruvate dehydrogenase kinase 1 and 2 deficiency reduces high-fat diet-induced hypertrophic obesity and inhibits the differentiation of preadipocytes into mature adipocytes Experimental & Molecular Medicine 2021-09-22 [PMID: 34552205] (Western Blot)	Western Blot
Nanduri J, Wang N, Wang Bl, Prabhakar Nr Lysine demethylase KDM6B regulates HIF-1 alpha mediated systemic and cellular responses to intermittent hypoxia Physiological genomics 2021-07-23 [PMID: 34297635]
Gaba RC, Khabbaz RC, Muchiri RN et al. Conventional versus drug-eluting embolic transarterial chemoembolization with doxorubicin: comparative drug delivery, pharmacokinetics, and treatment response in a rabbit VX2 tumor model Drug delivery and translational research 2021-04-16 [PMID: 33861419]
Guo Y, Zhou J, Li X et al. The Association of Suppressed Hypoxia-Inducible Factor-1 Transactivation of Angiogenesis With Defective Recovery From Cerebral Ischemic Injury in Aged Rats Frontiers in Aging Neuroscience 2021-02-26 [PMID: 33716719]
Kerry J, Specker E, Mizzoni M et al. Autophagy-dependent alternative splicing event produces a more stable ribosomal protein S24 isoform that aids in hypoxic cell survival bioRxiv 2023-09-26 (WB)	WB
Yeh JL, Kuo CH, Shih PW et al. Xanthine derivative KMUP-1 ameliorates retinopathy Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-07-03 [PMID: 37406513]
Bharti A, Urs AB, Kumar P. Significance of HIF-1? Expression and LOXL-2 Localization in Progression of Oral Squamous Cell Carcinoma Asian Pacific Journal of Cancer Prevention 2021-02-01 [PMID: 33639646] (Immunohistochemistry, Immunohistochemistry-Paraffin)	Immunohistochemistry, Immunohistochemistry-Paraffin
Kalainayakan SP, Ghosh P, Dey S et al. Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression Sci Rep 2019-02-07 [PMID: 30723259] (IHC-P, Mouse)	IHC-P	Mouse
Timpano S, Guild BD, Specker EJ et al. Physioxic human cell culture improves viability, metabolism, and mitochondrial morphology while reducing DNA damage. FASEB J. 2019-01-16 [PMID: 30649960] (WB, Mouse)	WB	Mouse
Melanson G Computational analysis implicates interferon-stimulated gene 15 in hypoxic ribonucleoprotein dynamics Thesis 2021-01-01 (WB, Human)	WB	Human
Schmid R, Schmidt S, Detsch R Et al. A New Printable Alginate/Hyaluronic Acid/Gelatin Hydrogel Suitable for Biofabrication of In Vitro and In Vivo Metastatic Melanoma Models Advanced Functional Materials 2021-10-13 (IF/IHC, Rat)	IF/IHC	Rat
Segura S, Stolnicu S, Boros M et al. mTOR Pathway Activation Assessed by Immunohistochemistry in Cervical Biopsies of HPV-associated Endocervical Adenocarcinomas (HPVA): Correlation With Silva Invasion Patterns Applied Immunohistochemistry & Molecular Morphology 2021-08-01 [PMID: 33587450] (Immunohistochemistry)	Immunohistochemistry
Wang N, Shi XF, Khan SA et al. Hypoxia inducible factor-1 mediates pancreatic beta-cell dysfunction by intermittent hypoxia Am. J. Physiol., Cell Physiol. 2020-09-16 [PMID: 32936698] (WB, Mouse)	WB	Mouse
Cai T, Ke Q, Fang Y et al. Sodium-glucose cotransporter 2 inhibition suppresses HIF-1alpha-mediated metabolic switch from lipid oxidation to glycolysis in kidney tubule cells of diabetic mice Cell Death Dis 2020-05-22 [PMID: 32444604] (ICC/IF, IHC-P, Human, Mouse)	ICC/IF, IHC-P	Human, Mouse
Lee, SM;Lee, KW;Kim, MA;Song, YS;Goo, JM;Park, CM; Serial Texture Analyses on ADC Maps for Evaluation of Antiangiogenic Therapy in Rat Breast Cancer Anticancer Res. 2019-04-01 [PMID: 30952728] (IF/IHC, Rat)	IF/IHC	Rat
Megele R, Riemenschneider MJ, Dodoo-Schittko F et al. Intra tumoral treatment with oxygen ozone in glioblastoma: A systematic literature search and results of a case series. Oncol Lett. [PMID: 30344733] (IF/IHC, Human)	IF/IHC	Human
Chen CW, Cheng TJ, Ho CH et al. Increased risk of brain cancer incidence in stroke patients: a clinical case series, population-based and longitudinal follow-up study Oncotarget 2017-12-12 [PMID: 29312585] (IHC-P, Human)	IHC-P	Human
Puchert M, Pelkner F, Stein G et al. Astrocytic expression of the CXCL12 receptor, CXCR7/ACKR3 is a hallmark of the diseased, but not developing CNS Mol. Cell. Neurosci. 2017-09-08 [PMID: 28889992] (Human)		Human
Mandl M, Depping R. ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells. Cancer Cell Int. 2017-08-31 [PMID: 28855849]
Wang L, Zheng Q, Yuan Y et al. Effects of 17b-estradiol and 2-methoxyestradiol on the oxidative stress-hypoxia inducible factor-1 pathway in hypoxic pulmonary hypertensive rats. Exp Ther Med 2017-05-01 [PMID: 28565876] (WB, Rat)	WB	Rat
Tong D, Liu Q, Liu G et al. The HIF/PHF8/AR axis promotes prostate cancer progression. Oncogenesis. 2016-12-19 [PMID: 27991916] (IP, Human)	IP	Human
Jablonska B, Gierdalski M, Chew LJ et al.. Sirt1 regulates glial progenitor proliferation and regeneration in white matter after neonatal brain injury. Nat Commun 2016-12-19 [PMID: 27991597] (KO, Mouse)	KO	Mouse
Timpano S, Uniacke J. Human Cells Cultured under Physiological Oxygen Utilize Two Cap-binding Proteins to recruit Distinct mRNAs for Translation. J Biol Chem 2016-05-13 [PMID: 27002144]
Fujimoto Y, Urashima T, Shimura D et al. Low Cardiac Output Leads Hepatic Fibrosis in Right Heart Failure Model Rats. PLoS One 2016-02-10 [PMID: 26863419] (WB)	WB
Favier F B, Britto F A et al. Endurance training prevents negative effects of the hypoxia mimetic dimethyloxalylglycine on cardiac and skeletal muscle function. J Appl Physiol (1985) 2016-02-15 [PMID: 26679609] (WB, Rat)	WB	Rat
Kim JI, Lee HJ, Goo JM et al. Correlation of volumetric perfusion CT parameters with hypoxia inducible factor-1 alpha expression in a rabbit VX2 tumor model. Acta Radiol 2015-09-02 [PMID: 26339038] (Rabbit)		Rabbit
Pang X, Wang Z, Yin D, Zhang Z. Overexpression of hypoxia-inducible factor prolyl- hydoxylase attenuated by HCG-induced vascular endothelial growth factor expression in luteal cells. Mol Med Rep 2015-05-16 [PMID: 25975603]
Henique C, Mansouri A, Vavrova E et al. Increasing mitochondrial muscle fatty acid oxidation induces skeletal muscle remodeling toward an oxidative phenotype FASEB J. 2015-02-23 [PMID: 25713059] (WB, Mouse)	WB	Mouse
Bruns Dr, Brown Rd, Stenmark Kr et al. Mitochondrial Integrity in a neonatal Bovine Model of Right Ventricular Dysfunction. Am. J. Physiol. Lung Cell Mol. Physiol. 2014-11-21 [PMID: 25416385]
Su Ej, Xin H, Yin P et al. Impaired fetoplacental angiogenesis in growth-restricted fetuses with abnormal umbilical artery Doppler velocimetry is mediated by aryl hydrocarbon receptor nuclear translocator (ARnT). J. Clin. Endocrinol. Metab. 2014-10-24 [PMID: 25343232] (IP, Human)	IP	Human
Niemi Henna, Honkonen Krista, Korpisalo Petra et al. HIF-1alpha and HIF-2alpha induce angiogenesis and improve muscle energy recovery. Eur J Clin Invest. 2014-10-01 [PMID: 25208310]
Motzer Robert J, Hutson Thomas E, Hudes Gary R et al. Investigation of novel circulating proteins, germ line single-nucleotide polymorphisms, and molecular tumor markers as potential efficacy biomarkers of first-line sunitinib therapy for advanced renal cell carcinoma. Cancer Chemother Pharmacol. 2014-08-07 [PMID: 25100134] (IHC-P, Human)	IHC-P	Human
Xiao W, Shinohara M, Komori K et al. The importance of physiological oxygen concentrations in the sandwich cultures of rat hepatocytes on gas-permeable membranes. Biotechnol. Prog. 2014-07-30 [PMID: 25078970]
Kaira K, Murakami H, Endo M et al. Biological Correlation of 18F-FDG Uptake on PET in Pulmonary Neuroendocrine Tumors. Anticancer Res. 2013-10-01 [PMID: 24122985]
Chaux A, Albadine R, Schultz L et al. Dysregulation of the mammalian target of rapamycin pathway in chromophobe renal cell carcinomas. Hum Pathol. 2013-08-15 [PMID: 23953228] (IHC-P, Human)	IHC-P	Human
Sceneay J, Liu MC, Chen A et al. The Antioxidant N-Acetylcysteine Prevents HIF-1 Stabilization under Hypoxia In Vitro but Does Not Affect Tumorigenesis in Multiple Breast Cancer Models In Vivo. PLoS One 2013-06-20 [PMID: 23840457] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
Pang X, Wang Z, Yin D, Zhang Z. Overexpression of hypoxia-inducible factor prolyl- hydoxylase attenuated by HCG-induced vascular endothelial growth factor expression in luteal cells. African Journal of Biotechnology 10(42):8227-8235.
Ohmoto T, Yoshitani N, Nishitsuji K et al. CD44-expressing undifferentiated carcinoma with rhabdoid features of the pancreas: Molecular analysis of aggressive invasion and metastasis. Pathol. Int. 2015-03-09 [PMID: 25753521] (IHC-P, Human)	IHC-P	Human
Ramamoorthy Prabhu, Shi Honglian. Ischemia induces different levels of hypoxia inducible factor-1alpha protein expression in interneurons and pyramidal neurons. Acta Neuropathol Commun. 2014-05-05 [PMID: 24887017] (ICC/IF, Rat)	ICC/IF	Rat
Obama T, Takayanagi T, Kobayashi T et al. Vascular induction of a disintegrin and metalloprotease 17 by angiotensin II through hypoxia inducible factor 1alpha. Am J Hypertens 2015-01-01 [PMID: 24871629]
Sun W, Depping R, Jelkmann W. Interleukin-1beta promotes hypoxia-induced apoptosis of glioblastoma cells by inhibiting hypoxia-inducible factor-1 mediated adrenomedullin production. Cell Death Dis. 2014-01-23 [PMID: 24457964] (WB, Human)	WB	Human
Li C, Hashimi SM, Cao S et al. The mechanisms of chansu in inducing efficient apoptosis in colon cancer cells. Evid Based Complement Alternat Med 2013-01-01 [PMID: 23818933] (ICC/IF, Human)	ICC/IF	Human
Li J, Levick SP, Dipette DJ et al. Alpha-calcitonin gene-related peptide is protective against pressure overload-induced heart failure. Regul Pept 2013-06-28 [PMID: 23816470] (WB, Mouse)	WB	Mouse
Tanaka T, Yamaguchi J, Higashijima Y, Nangaku M. Indoxyl sulfate signals for rapid mRNA stabilization of Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (CITED2) and suppresses the expression of hypoxia-inducible genes in experimental CKD and uremia. FASEB J 2013-06-21 [PMID: 23792300] (WB, Chemotaxis, Human)	WB, Chemotaxis	Human
Yang Chunmei, Lee Heehyoung, Pal Sumanta et al. B cells promote tumor progression via STAT3 regulated-angiogenesis. PLoS One 2013-01-01 [PMID: 23734190] (WB, Mouse)	WB	Mouse
Toba H, Kondo K, Sadohara Y et al. 18F-fluorodeoxyglucose positron emission tomography/computed tomography and the relationship between fluorodeoxyglucose uptake and the expression of hypoxia-inducible factor-1alpha, glucose transporter-1 and vascular endothelial growth factor in thymic epithelial tumours. Eur J Cardiothorac Surg 2013-05-14 [PMID: 23674658] (IHC-P, Human)	IHC-P	Human
Kar AN, Macgibeny MA, Gervasi NM et al. Intra-axonal Synthesis of Eukaryotic Translation Initiation Factors Regulates Local Protein Synthesis and Axon Growth in Rat Sympathetic Neurons. J Neurosci 2013-04-24 [PMID: 23616526] (WB, Rat)	WB	Rat
Lundgrin EL, Park MM, Sharp J et al. Fasting 2-Deoxy-2-[18F]fluoro-D-glucose Positron Emission Tomography to Detect Metabolic Changes in Pulmonary Arterial Hypertension Hearts over 1 Year. Ann Am Thorac Soc 2013-02-01 [PMID: 23509326] (IHC-P, Human)	IHC-P	Human
Aziz SA, Sznol JA, Adeniran A et al. Expression of drug targets in primary and matched metastatic renal cell carcinoma tumors. BMC Clin Pathol 2013-02-01 [PMID: 23374878] (IF/IHC, Human)	IF/IHC	Human
Reddy VD, Padmavathi P, Kavitha G et al. Alcohol-induced oxidative/nitrosative stress alters brain mitochondrial membrane properties Mol Cell Biochem 2012-12-01 [PMID: 23212448] (WB, Rat)	WB	Rat
Brenner RM, Slayden OD. Molecular and functional aspects of menstruation in the macaque Rev Endocr Metab Disord 2012-10-30 [PMID: 23108498] (IF/IHC, Primate)	IF/IHC	Primate
Ioannou M, Pyrpasopoulou A, Simos G et al. Upregulation of VEGF expression is associated with accumulation of HIF-1 alpha in the skin of naive scleroderma patients Mod Rheumatol 2012-10-25 [PMID: 23096096] (IF/IHC, Human)	IF/IHC	Human
Tirziu D, Jaba IM, Yu P et al. Endothelial nuclear factor-kB-dependent regulation of arteriogenesis and branching Circulation. 2012-11-27 [PMID: 23091063] (WB, Human)	WB	Human
Shinmei S, Sakamoto N, Goto K et al. MicroRNA-155 is a predictive marker for survival in patients with clear cell renal cell carcinoma Int J Urol 2012-10-10 [PMID: 23050614] (IF/IHC, Human)	IF/IHC	Human
Salem AF, Howell A, Sartini M. Downregulation of stromal BRCA1 drives breast cancer tumor growth via upregulation of HIF-1 alpha, autophagy and ketone body production. Cell Cycle. 2012-11-01 [PMID: 23047605] (WB, Human)	WB	Human
Clarke DN, Al Ahmad A, Lee B et al. Perlecan Domain V Induces VEGf Secretion in Brain Endothelial Cells through Integrin alpha5beta1 and ERK-Dependent Signaling Pathways PLoS One 2012-01-01 [PMID: 23028886] (WB, Mouse)	WB	Mouse
Ding A, Kalaignanasundaram P, Ricardo SD et al. Chronic treatment with tempol does not significantly ameliorate renal tissue hypoxia or disease progression in a rodent model of polycystic kidney disease Clin Exp Pharmacol Physiol 2012-09-25 [PMID: 23006058] (IF/IHC, ICC/IF, Rat)	IF/IHC, ICC/IF	Rat
Gu HF, Zheng X, Abu Seman N et al. Impact of the Hypoxia-Inducible Factor-1 alpha (HIF1A) Pro582Ser Polymorphism on Diabetes Nephropathy. Diabetes Care 2013-02-01 [PMID: 22991450] (IHC-P, Mouse)	IHC-P	Mouse
Zhao Y, Xiong X, Jia L, Sun Y. Targeting Cullin-RING ligases by MLN4924 induces autophagy via modulating the HIF1-REDD1-TSC1-mTORC1-DEPTOR axis Cell Death Dis 2012-09-06 [PMID: 22951983] (WB, Human)	WB	Human
Mhawech-Fauceglia P, Wang D, Samrao D et al. The role of hypoxic-inducible factor (HIF1alpha) and aldolaseC protein in endometrial carcinogenesis: a retrospective study of 279 patients BMJ Open 2012-08-24 [PMID: 22923632] (IF/IHC, Human)	IF/IHC	Human
Chiavarina B, Martinez-Outschoorn UE, Whitaker-Menezes D et al. Metabolic reprogramming and two-compartment tumor metabolism: Opposing role(s) of HIF1alpha and HIF2alpha in tumor-associated fibroblasts and human breast cancer cells Cell Cycle 2012-09-01 [PMID: 22894905] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Mayer A, Schneider F, Vaupel P et al. Differential expression of HIF-1 in glioblastoma multiforme and anaplastic astrocytoma. Int J Oncol 2012-10-01 [PMID: 22825389] (IF/IHC, Human)	IF/IHC	Human
Kishimoto K, Yoshida S, Ibaragi S et al. Hypoxia-induced up-regulation of angiogenin, besides VEGF, is related to progression of oral cancer Oral Oncol 2012-06-11 [PMID: 22694909] (WB, IF/IHC, Human)	WB, IF/IHC	Human
Jiang W, Zhu Z, McGinley JN et al. Identification of a molecular signature underlying inhibition of mammary carcinoma growth by dietary N-3 Fatty acids Cancer Res 2012-08-01 [PMID: 22651929] (WB, Rat)	WB	Rat
Galzio R, Cristiano L, Fidoamore A et al. Hypoxia modulation of peroxisome proliferator-activated receptors (PPARs) in human glioblastoma stem cells. Implications for therapy J Cell Biochem 2012-11-01 [PMID: 22644833] (ICC/IF, FLOW, Human)	ICC/IF, FLOW	Human
Weber DC, Tille JC, Combescure C et al. The prognostic value of expression of HIF1alpha, EGFR and VEGF-A, in localized prostate cancer for intermediate- and high-risk patients treated with radiation therapy with or without androgen deprivation therapy Radiat Oncol 2012-04-30 [PMID: 22546016] (IF/IHC, Human)	IF/IHC	Human
Chaux A, Schultz L, Albadine R et al. Immunoexpression status and prognostic value of mammalian target of rapamycin and hypoxia-induced pathway members in papillary cell renal cell carcinomas Hum Pathol 2012-12-01 [PMID: 22542128] (IF/IHC, Human)	IF/IHC	Human
Jeong CW, Yoon CY, Jeong SJ, Hong SK, Byun SS, Kwak C, Lee SE. The Role of Hypoxia-Inducible Factor-1? and -2? in Androgen Insensitive Prostate Cancer Cells. Urol Oncol. 2012-04-24 [PMID: 22537539] (WB, Human)	WB	Human
Kaira K, Serizawa M, Koh Y, Takahashi T, Hanaoka H, Oriuchi N, Endo M, Kondo H, Nakajima T, Yamamoto N. Relationship between (18)F-FDG uptake on positron emission tomography and molecular biology in malignant pleural mesothelioma. Eur J Cancer. 2012-02-11 [PMID: 22330319] (WB, IHC-P, Human)	WB, IHC-P	Human
Holscher M, Schafer K, Krull S, Farhat K, Hesse A, Silter M, Lin Y, Pichler BJ, Thistlethwaite P, El-Armouche A, Maier LS, Katschinski DM, Zieseniss A. Unfavourable consequences of chronic cardiac HIF-1? stabilization. Cardiovasc Res. 2012-02-20 [PMID: 22258630] (IHC-P, Mouse)	IHC-P	Mouse
Kaira K, Oriuchi N, Takahashi T et al. L-type amino acid transporter 1 (LAT1) expression in malignant pleural mesothelioma. Anticancer Res 2011-12-01 [PMID: 22199264] (IF/IHC, Human)	IF/IHC	Human
Chu W, Wan L, Zhao D, Qu X, Cai F, Huo R, Wang N, Zhu J, Zhang C, Zheng F, Cai R, Dong D, Lu Y, Yang B. Mild hypoxia induced cardiomyocyte hypertrophy via up-regulation of HIF-1?-mediated TRPC signaling. J Cell Mol Med. 2011-11-30 [PMID: 22129453] (ICC/IF, WB, Rat)	ICC/IF, WB	Rat
Kaira K, Nakagawa K, Ohde Y et al. Depolarized MUC1 expression is closely associated with hypoxic markers and poor outcome in resected non-small cell lung cancer. Int J Surg Pathol 2012-06-01 [PMID: 22108499] (Human)		Human
Kaira K, Abe M, Nakagawa K et al. (18)F-FDG uptake on PET in primary mediastinal non-thymic neoplasm: A clinicopathological study. Eur J Radiol. 2011-11-03 [PMID: 22055682] (IF/IHC, Human)	IF/IHC	Human
Kaira K, Oriuchi N, Takahashi T et al. LAT1 expression is closely associated with hypoxic markers and mTOR in resected non-small cell lung cancer. Am J Transl Res 2011-01-01 [PMID: 22046488] (IF/IHC)	IF/IHC
Kaira K, Okumura T, Nakagawa K et al. MUC1 Expression in Pulmonary Metastatic Tumors: A Comparison of Primary Lung Cancer Pathol Oncol Res. 2012-04-18 [PMID: 21964914] (IF/IHC, Human)	IF/IHC	Human
Kaira K, Ohde Y, Nakagawa K et al. Thymidylate synthase expression is closely associated with outcome in patients with pulmonary adenocarcinoma Med Oncol 2011-01-01 [PMID: 21948461] (IF/IHC, Human)	IF/IHC	Human
Sreekumar PG, Hinton DR, Kannan R. Methionine sulfoxide reductase A: Structure, function and role in ocular pathology. World J Biol Chem 262(8):184-192. 2011-08-01 [PMID: 21909460] (WB, Human)	WB	Human
Schultz L, Chaux A, Albadine R et al. Immunoexpression status and prognostic value of mTOR and hypoxia-induced pathway members in primary and metastatic clear cell renal cell carcinomas. Am J Surg Pathol 2011-10-01 [PMID: 21881486] (Human)		Human
Tandon R, SenGupta D, Ndhlovu LC et al. Identification of human endogenous retrovirus-specific T cell responses in vertically HIV-1-infected subjects. J Virol 2011 Nov [PMID: 21880743] (WB)	WB
Peurala E, Koivunen P, Bloigu R, Haapasaari KM, Jukkola-Vuorinen A. Expressions of individual PHDs associate with good prognostic factors and increased proliferation in breast cancer patients. Breast Cancer Res Treat;133(1):179-88. 2012-05-01 [PMID: 21877141] (IF/IHC, Human)	IF/IHC	Human
Schneider C, Krischke G, Rascher W et al. Systemic hypoxia differentially affects neurogenesis during early mouse brain maturation. Brain Dev. 2011-08-06 [PMID: 21824737] (WB, Mouse)	WB	Mouse
Kaira K, Ohde Y, Endo M et al. Expression of 4F2hc (CD98) in pulmonary neuroendocrine tumors. Oncol Rep 2011-10-01 [PMID: 21750865] (IF/IHC, Human)	IF/IHC	Human
Monzon FA, Alvarez K, Peterson L et al. Chromosome 14q loss defines a molecular subtype of clear-cell renal cell carcinoma associated with poor prognosis. Mod Pathol 2011-11-01 [PMID: 21725288] (IF/IHC, Human)	IF/IHC	Human
Evenou F, Hamon M, Fujii T et al. Gas?permeable membranes and co?culture with fibroblasts enable high?density hepatocyte culture as multilayered liver tissues. Biotechnology Progress 2011-01-01 [PMID: 21630487] (IHC-P, ICC/IF, Mouse)	IHC-P, ICC/IF	Mouse
Namas RA, Metukuri MR, Dhupar R et al. Hypoxia-induced overexpression of BNIP3 is not dependent on hypoxia-inducible factor 1? in mouse hepatocytes. Shock. 2011-08-01 [PMID: 21558981] (WB, Mouse)	WB	Mouse
Park HJ, Baek KH, Lee HL et al. Hypoxia inducible factor-1alpha directly induces the expression of receptor activator of nuclear factor-?B ligand in periodontal ligament fibroblasts. Mol Cells;31(6):573-8. 2011-06-01 [PMID: 21533547] (WB, Human)	WB	Human
Weroha SJ, Oberg AL, Ziegler KL et al. Phase II trial of lapatinib and topotecan (LapTop) in patients with platinum-refractory/resistant ovarian and primary peritoneal carcinoma Gynecol Oncol 2011-07-01 [PMID: 21514634] (IF/IHC, Human)	IF/IHC	Human
Kaira K, Okumura T, Ohde Y et al. Correlation Between 18F-FDG Uptake on PET and Molecular Biology in Metastatic Pulmonary Tumors. J Nucl Med. 2011-04-15 [PMID: 21498541] (IHC-P, Human)	IHC-P	Human
Huang LJ, Chuang IC, Dong HP, Yang RC. Hypoxia-inducible factor 1alpha regulates the expression of the mitochondrial ATPase inhibitor protein (IF1) in rat liver. Shock;36(1):90-6. 2011-07-01 [PMID: 21412184] (WB, Rat)	WB	Rat
Hoelscher M, Silter M, Krull S et al. Cardiomyocyte-specific Prolyl-4-hydroxylase domain 2 knock out protects from acute myocardial ischaemic injury. J Biol Chem. 2011-01-01 [PMID: 21270129] (IHC-P, Mouse)	IHC-P	Mouse
Gibbings S, Elkins ND, Fitzgerald H et al. Xanthine oxidoreductase promotes the inflammatory state of mononuclear phagocytes through effects on chemokine expression, PPAR{gamma} sumoylation, and HIF-1{alpha}. J Biol Chem. 2010-11-08 [PMID: 21059659] (WB, Rat)	WB	Rat
Chiavarina B, Whitaker-Menezes D, Migneco G et al. HIF1-alpha functions as a tumor promoter in cancer associated fibroblasts, and as a tumor suppressor in breast cancer cells: Autophagy drives compartment-specific oncogenesis. Cell Cycle 2010-09-01 [PMID: 20864819] (ICC/IF)	ICC/IF
Martinez-Outschoorn UE, Trimmer C, Lin Z et al. Autophagy in cancer associated fibroblasts promotes tumor cell survival: Role of hypoxia, HIF1 induction and NFkappaB activation in the tumor stromal microenvironment. Cell Cycle 2010-09-01 [PMID: 20855962] (ICC/IF)	ICC/IF
Tickoo SK, Milowsky MI, Dhar N et al. Hypoxia-inducible factor and mammalian target of rapamycin pathway markers in urothelial carcinoma of the bladder: possible therapeutic implications. BJU Int 2011-03-01 [PMID: 20707797]
Monsef N, Soller M, Panagopoulos I, Abrahamsson PA. HIF1alpha isoforms in benign and malignant prostate tissue and their correlation to neuroendocrine differentiation. BMC Cancer 2010-01-01 [PMID: 20663134] (IF/IHC, Human)	IF/IHC	Human
Kaira K, Endo M, Abe M et al. Biologic correlates of F-FDG uptake on PET in pulmonary pleomorphic carcinoma Lung Cancer 2011-02-01 [PMID: 20646779] (IF/IHC, Human)	IF/IHC	Human
Kaira K, Endo M, Abe M et al. Biologic correlation of 2-[18F]-fluoro-2-deoxy-D-glucose uptake on positron emission tomography in thymic epithelial tumors. J Clin Oncol 2010-08-10 [PMID: 20625125] (IF/IHC, Human)	IF/IHC	Human
Ioannou M, Mylonis I, Kouvaras E et al. Validated analysis of HIF-1alpha expression in cancer cells using a controlled and comparative immunoassay. Oncol Rep 2010-07-01 [PMID: 20514457] (WB, Human)	WB	Human
Choudhury KR, Yagle KJ, Swanson PE et al. A robust automated measure of average antibody staining in immunohistochemistry images. J Histochem Cytochem 2010-02-01 [PMID: 19687472] (IF/IHC, Human)	IF/IHC	Human
Lee DW, Rajagopalan S, Siddiq A et al. Inhibition of Prolyl Hydroxylase Protects against 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Neurotoxicity: MODEL FOR THE POTENTIAL INVOLVEMENT OF THE HYPOXIA-INDUCIBLE FACTOR PATHWAY IN PARKINSON DISEASE. J Biol Chem;284(42):29065-29076. 2009-01-01 [PMID: 19679656] (WB, ICC/IF, IHC-P, Mouse)	WB, ICC/IF, IHC-P	Mouse
Evenou F, Fujii T, Sakai Y. Spontaneous formation of highly functional three-dimensional multilayer from human hepatoma Hep G2 cells cultured on an oxygen-permeable polydimethylsiloxane membrane. Tissue Eng Part C Methods 2010-04-01 [PMID: 19563254] (IF/IHC, Human)	IF/IHC	Human
Aubert S, Fauquette V, Hemon B et al. MUC1, a New Hypoxia Inducible Factor Target Gene, Is an Actor in Clear Renal Cell Carcinoma Tumor Progression. Cancer Res;69(14):5707-5715. 2009-01-01 [PMID: 19549898] (GS, IHC-P, WB, Human)	GS, IHC-P, WB	Human
Rhoads RP, Johnson RM, Rathbone CR et al. Satellite cell-mediated angiogenesis coincides with a functional hypoxia-inducible factor (HIF) pathway. Am J Physiol Cell Physiol :00391. 2009-01-01 [PMID: 19386789] (ICC/IF, WB, Rat)	ICC/IF, WB	Rat
Gossl M, Versari D, Lerman LO et al. Low vasa vasorum densities correlate with inflammation and subintimal thickening: potential role in location--determination of atherogenesis. Atherosclerosis 2009-10-01 [PMID: 19368925] (IF/IHC, Porcine)	IF/IHC	Porcine
Hatipoglu OF, Hirohata S, Cilek MZ et al. ADAMTS1 Is a Unique Hypoxic Early Response Gene Expressed by Endothelial Cells. J Biol Chem;284(24):16325-16333. 2009-01-01 [PMID: 19349275] (Chemotaxis, Human)	Chemotaxis	Human
Virmani S, Rhee TK, Ryu RK et al. Comparison of hypoxia-inducible factor-1alpha expression before and after transcatheter arterial embolization in rabbit VX2 liver tumors. J Vasc Interv Radiol 2008-10-01 [PMID: 18922400] (IF/IHC)	IF/IHC
Stagg J, Sharkey J, Pommey S, Young R, Takeda K, Yagita H, Johnstone RW, Smyth MJ. Antibodies targeted to TRAIL receptor-2 and ErbB-2 synergize in vivo and induce an antitumor immune response. Proc Natl Acad Sci U S A;105(42):16254-9. 2008-10-21 [PMID: 18838682] (IHC-P, Mouse)	IHC-P	Mouse
Flynn JR, Wang L, Gillespie DL et al. Hypoxia-regulated protein expression, patient characteristics, and preoperative imaging as predictors of survival in adults with glioblastoma multiforme. Cancer 2008-09-01 [PMID: 18618497] (IHC-P, Human)	IHC-P	Human
Rosenberger C, Rosen S, Shina A et al. Activation of hypoxia-inducible factors ameliorates hypoxic distal tubular injury in the isolated perfused rat kidney. Nephrol Dial Transplant;23(11):3472-8. 2008-11-01 [PMID: 18515655] (IHC-P, Rat)	IHC-P	Rat
Radek KA, Kovacs EJ, Gallo RL, DiPietro LA. Acute Ethanol Exposure Disrupts VEGF Receptor Cell Signaling in Endothelial Cells. Am J Physiol Heart Circ Physiol. :00699.2007. 2008-05-09 [PMID: 18469146] (WB, Rat, Mouse)	WB	Rat, Mouse
Diensthuber M, Potinius M, Rodt T et al. Expression of bcl-2 is associated with microvessel density in olfactory neuroblastoma. J Neurooncol 2008-09-01 [PMID: 18431543] (IHC-P)	IHC-P
Lopez-Aviles S, Lambea E, Moldon A et al. Activation of Srk1 by the mitogen-activated protein kinase Sty1/Spc1 precedes its dissociation from the kinase and signals its degradation. Mol Biol Cell 2008-04-01 [PMID: 18272791] (ELISA)	ELISA
Klatte, T et al. Hypoxia-inducible factor 1 alpha in clear cell renal cell carcinoma. Clin Cancer Res;13(24):7388-93. 2007-12-15 [PMID: 18094421] (IHC-P, Human)	IHC-P	Human
Cabuk D, Basaran G, Celikel C et al. Vascular endothelial growth factor, hypoxia-inducible factor 1 alpha and CD34 expressions in early-stage gastric tumors: relationship with pathological factors and prognostic impact on survival. Oncology 2007-01-01 [PMID: 18025805] (Human)		Human
Yamamoto Y, Ibusuki M, Okumura Y, Kawasoe T, Kai K, Iyama K, Iwase H. Hypoxia-inducible factor 1alpha is closely linked to an aggressive phenotype in breast cancer. Breast Cancer Res Treat;110(3):465-75. 2008-08-01 [PMID: 17805961] (IHC-P, Human)	IHC-P	Human
Tracy K, Dibling BC, Spike BT, Knabb JR, Schumacker P, Macleod KF. BNIP3 is an RB/E2F target gene required for hypoxia-induced autophagy. Mol Cell Biol;27(17):6229-42. 2007-09-01 [PMID: 17576813] (Chemotaxis, GS, Mouse)	Chemotaxis, GS	Mouse
Monsef N, Helczynski L, Lundwall A, Pahlman S et al. Localization of immunoreactive HIF-1alpha and HIF-2alpha in neuroendocrine cells of both benign and malignant prostate glands. Prostate 2007-08-01 [PMID: 17562539] (ICC/IF, Human)	ICC/IF	Human
Anderson KM, Tsui P, Guinan P et al. The proliferative response of hela cells to 2-deoxy-D-glucose under hypoxic or anoxic conditions: an analogue for studying some properties of in vivo solid cancers. Anticancer Res 2006-11-01 [PMID: 17201127] (IF/IHC, WB, Human)	IF/IHC, WB	Human
Gagnon PM, Simmons DD, Bao J et al. Temporal and genetic influences on protection against noise-induced hearing loss by hypoxic preconditioning in mice. Hear Res 2007-04-01 [PMID: 17107766] (WB, Mouse)	WB	Mouse
Ramanathan M, Pinhal-Enfield G, Hao I et al. Synergistic up-regulation of vascular endothelial growth factor (VEGF) expression in macrophages by adenosine A2A receptor agonists and endotoxin involves transcriptional regulation via the hypoxia response element in the VEGF promoter. Mol Biol Cell 18(1):14-23. 2007-01-01 [PMID: 17065555] (ELISA, Human, Mouse)	ELISA	Human, Mouse
Khan Z, Michalopoulos GK, Stolz DB. Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation. Am J Pathol 2006-10-01 [PMID: 17003483] (ICC/IF, IHC-P, Rat)	ICC/IF, IHC-P	Rat
Isaacs JT, Pili R, Qian DZ et al. Identification of ABR-215050 as lead second generation quinoline-3-carboxamide anti-angiogenic agent for the treatment of prostate cancer. Prostate 2006-12-01 [PMID: 16955399] (ICC/IF)	ICC/IF
An HJ, Maeng O, Kang KH, Lee JO, Kim YS, Paik SG, Lee H. Activation of Ras up-regulates pro-apoptotic BNIP3 in nitric oxide-induced cell death. J Biol Chem;281(45):33939-48. 2006-11-10 [PMID: 16954213] (WB, Human, Mouse)	WB	Human, Mouse
Laderoute KR, Amin K, Calaoagan JM, Knapp M, Le T, Orduna J, Foretz M, Viollet B. 5'-AMP-activated protein kinase (AMPK) is induced by low-oxygen and glucose deprivation conditions found in solid-tumor microenvironments. Mol Cell Biol;26(14):5336-47. 2006-07-01 [PMID: 16809770] (WB, Mouse)	WB	Mouse
Zheng X, Ruas JL, Cao R et al. Cell-type-specific regulation of degradation of hypoxia-inducible factor 1a: Role of subcellular compartmentalization. Mol Cell Biol 2006-06-01 [PMID: 16738327] (IP, WB, Rabbit)	IP, WB	Rabbit
Kim CM, Vocke C, Torres-Cabala C et al. Expression of hypoxia inducible factor-1alpha and 2alpha in genetically distinct early renal cortical tumors. J Urol 2006-05-01 [PMID: 16600797] (Human)		Human
Franco M, Man S, Chen L et al. Targeted anti-vascular endothelial growth factor receptor-2 therapy leads to short-term and long-term impairment of vascular function and increase in tumor hypoxia. Cancer Res 2006-04-01 [PMID: 16585189] (IHC-P, WB, Human)	IHC-P, WB	Human
Michelsen J, Thiesson H, Walter S et al. Tissue expression and plasma levels of adrenomedullin in renal cancer patients. Clin Sci (Lond) 2006-07-01 [PMID: 16515534] (IF/IHC, Human)	IF/IHC	Human
Lu H et al. Reversible inactivation of HIF-1 prolyl hydroxylases allows cell metabolism to control basal HIF-1. J Biol Chem;280(51):41928-39. 2005-12-23 [PMID: 16223732] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Murphy BJ, Sato BG, Dalton TP, Laderoute KR. The metal-responsive transcription factor-1 contributes to HIF-1 activation during hypoxic stress. Biochem Biophys Res Commun 2005-11-25 [PMID: 16216223] (WB, Mouse)	WB	Mouse
Isaacs JS, Jung YJ, Mole DR et al. HIF overexpression correlates with biallelic loss of fumarate hydratase in renal cancer: novel role of fumarate in regulation of HIF stability. Cancer Cell 2005-08-01 [PMID: 16098467] (ELISA, Human)	ELISA	Human
Gross J, Fuchs J, Machulik A et al. Apoptosis, necrosis and hypoxia inducible factor-1 in human head and neck squamous cell carcinoma cultures. Int J Oncol 2005-09-01 [PMID: 16077932] (Human)		Human
Brown NS, Streeter EH, Jones A et al. Cooperative stimulation of vascular endothelial growth factor expression by hypoxia and reactive oxygen species: the effect of targeting vascular endothelial growth factor and oxidative stress in an orthotopic xenograft model of bladder carcinoma. Br J Cancer 2005-05-09 [PMID: 15841086] (WB, Rat)	WB	Rat
Li YM, Zhou BP, Deng J et al. A hypoxia-independent hypoxia-inducible factor-1 activation pathway induced by phosphatidylinositol-3 kinase/Akt in HER2 overexpressing cells. Cancer Res 2005-04-15 [PMID: 15833858]
Luyendyk JP, Maddox JF, Green CD et al. Role of hepatic fibrin in idiosyncrasy-like liver injury from lipopolysaccharide-ranitidine coexposure in rats. Hepatology 2004-12-01 [PMID: 15565632] (IF/IHC, Rat)	IF/IHC	Rat
Caron A, Desrosiers RR, Beliveau R. Kidney ischemia-reperfusion regulates expression and distribution of tubulin subunits, beta-actin and rho GTPases in proximal tubules. Arch Biochem Biophys 2004-11-01 [PMID: 15464724] (Rat)		Rat
Tezel G, Wax MB. Hypoxia-inducible factor 1alpha in the glaucomatous retina and optic nerve head. Arch Ophthalmol 2004-09-01 [PMID: 15364715] (IHC-P, Human)	IHC-P	Human
Cangul H. Hypoxia upregulates the expression of the NDRG1 gene leading to its overexpression in various human cancers. BMC Genet 2004-09-02 [PMID: 15341671] (IF/IHC, Human)	IF/IHC	Human
Nordal RA, Nagy A, Pintilie M, Wong CS. Hypoxia and hypoxia-inducible factor-1 target genes in central nervous system radiation injury: a role for vascular endothelial growth factor. Clin Cancer Res 2004-05-15 [PMID: 15161688] (ELISA)	ELISA
Reichner JS, Albina JE. Determination of the role of hypoxia-inducible factor 1 in wound healing. Methods Enzymol 2004-01-01 [PMID: 15063696] (Rat)		Rat
Bernaudin M, Sharp FR. Methods to detect hypoxia-induced ischemic tolerance in the brain. Methods Enzymol 2004-01-01 [PMID: 15063689] (WB)	WB
Uchida T, Rossignol F, Matthay MA et al. Prolonged hypoxia differentially regulates hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha expression in lung epithelial cells: implication of natural antisense HIF-1alpha. J Biol Chem 2004-04-09 [PMID: 14744852] (ICC/IF, Human)	ICC/IF	Human
de Candia, P et al. Angiogenesis impairment in Id-deficient mice cooperates with an Hsp90 inhibitor to completely suppress HER2/neu-dependent breast tumors. Proc Natl Acad Sci U S A;100(21):12337-42. 2003-10-14 [PMID: 14526102] (IHC-P, Mouse)	IHC-P	Mouse
Burke, B et al. Hypoxia-induced gene expression in human macrophages: implications for ischemic tissues and hypoxia-regulated gene therapy. Am J Pathol;163(4):1233-43. 2003-10-01 [PMID: 14507633] (IF/IHC, Human)	IF/IHC	Human
Seagroves TN, Hadsell D, McManaman J et al. HIF1alpha is a critical regulator of secretory differentiation and activation, but not vascular expansion, in the mouse mammary gland. Development 2003-04-01 [PMID: 12620994] (WB)	WB
Mandriota SJ et al. HIF activation identifies early lesions in VHL kidneys: evidence for site-specific tumor suppressor function in the nephron. Cancer Cell;1(5):459-68. 2002-06-01 [PMID: 12124175] (IHC-P, Human)	IHC-P	Human
Fujii, T et al. Effect of hypoxia on human seminoma cells. Int J Oncol;20(5):955-62. 2002-05-01 [PMID: 11956589] (ICC/IF, Human)	ICC/IF	Human
Tang T, Arbiser JL, Brandt SJ. Phosphorylation by mitogen-activated protein kinase mediates the hypoxia-induced turnover of the TAL1/SCL transcription factor in endothelial cells. J Biol Chem;277(21):18365-72. 2002-05-24 [PMID: 11904294] (WB, Human)	WB	Human
Albina JE, Mastrofrancesco B, Vessella JA et al. HIF-1 expression in healing wounds: HIF-1alpha induction in primary inflammatory cells by TNF-alpha. Am J Physiol Cell Physiol 2001-12-01 [PMID: 11698256] (Rat)		Rat
Duyndam, MCA et al. Induction of vascular endothelial growth factor expression and hypoxia-inducible factor 1alpha protein by the oxidative stressor arsenite. J Biol Chem;276(51):48066-76. 2001-12-21 [PMID: 11641398] (WB, Human)	WB	Human
Tacchini L, Dansi P, Matteucci E, Desiderio MA. Hepatocyte growth factor signalling stimulates hypoxia inducible factor-1 (HIF-1) activity in HepG2 hepatoma cells. Carcinogenesis;22(9):1363-71. 2001-09-01 [PMID: 11532856] (GS, WB, Human)	GS, WB	Human
Zhong, H et al. Overexpression of hypoxia-inducible factor 1alpha in common human cancers and their metastases. Can. Res.1999; 59: 5830-5835. 1999-01-01 [PMID: 10582706] (WB, IHC-P, Mouse, Human)	WB, IHC-P	Mouse, Human
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Reviews for HIF-1 alpha Antibody (NB100-123) (9) 4.29

Average Rating: 4.2

(Based on 9 reviews)

We have 9 reviews tested in 3 species: Human, Rat, Other.

Reviews using NB100-123:

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Human

12/12/2014

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Application	Western Blot
Sample Tested	See PMID:23091063
Species	Human

reviewed by:
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Other

07/15/2014

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Application	Western Blot
Sample Tested	Rat liver total lysate
Species	Other
Lot	R3

Immunoprecipitation HIF-1 alpha NB100-123

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Human

04/28/2014

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Application	Immunoprecipitation
Species	Human

reviewed by:
Verified Customer

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04/25/2014

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Application	Western Blot
Sample Tested	Mouse brain cortex whole lysate
Species	Other
Lot	R3

Immunohistochemistry HIF-1 alpha NB100-123

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reviewed by:
Ewa Kaniewska

IHC

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04/14/2014

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Application	Immunohistochemistry
Species	Other

reviewed by:
Ewa Kaniewska

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04/03/2014

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Application	Immunofluorescence
Species	Other

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reviewed by:
Prabir Chakraborty

Human

01/24/2014

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Application	Western Blot
Sample Tested	A2780 CELL LYSATE
Species	Human
Lot	S-3

Immunohistochemistry-Paraffin HIF-1 alpha NB100-123

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IHC-P

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05/15/2012

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Application	Immunohistochemistry-Paraffin
Sample Tested	human lung
Species	Human

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ICC

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03/24/2009

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Application	Immunocytochemistry
Sample Tested	Rat Primary Motor Neurons
Species	Rat
Lot	M

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FAQs for HIF-1 alpha Antibody (NB100-123). (Showing 1 - 10 of 13 FAQs).

Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?
- HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.
Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
- All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, we suggest the following four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products have been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449) (cited in at least 31 peer reviewed publications).
I would like to know, does a path exist for detection of HIF 1 in venous blood before and after revascularization of the leg?
- We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present, but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.
What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
- The theoretical molecular weight of HIF 1-alpha is ~93kDa. However, you will likely see a band between 100-120kDa due to phosphorylation.
We got the Hif1a (NB100-105) antibody from you guys. I used the concentration that is mentioned on your website, but I am getting a band of a completely different size (~70kDa) and not the 120 kDa mentioned.
- HIF-1 alpha is a notoriously difficult protein to work with due to its rapid degradation. Therefore, the ~70kDa bands are most likely degradation products. It is very important to lyse the cells in hypoxic conditions. We strongly recommend lysing the cells directly into the Laemmli buffer and doing that quickly, so that the exposure to oxygen is minimized.Please go through our hypoxia related FAQs, you should find them very informative.Also, running a positive control may help confirm the band specificity in your samples. You may prepare them yourself or choose some from our catalog, for example: 1) HeLa Hypoxic / Normoxic Cell Lysate (NBP2-36452)2) HeLa Hypoxic (CoCl2) / Normoxic Cell Lysate (NBP2-36450)
I performed several Western Blots of HIF-1 alpha with different lysis buffers, whole lysates, and cytoplasm/nuclei extractions. I can’t seem to get a good western blot (poor signal, band much lower than expected, etc.). Can someone suggest some technical considerations/tricks I should consider using?
- A major issue that researchers working with HIF-1 alpha is degradation due to exposure to oxygen. In western blot, this results in a weaker band and/or the appearance of multiple low molecular weight bands (40-80 kDa). We recommend preparing the lysates after collection of cells/tissues as quickly as possible (on ice), preferably in a hypoxic chamber. We also recommend including a true hypoxia mimetic control (eg: cells treated with CoCl2, DMOG… etc.). The controls help distinguish your band of interest from potential degradation/dimer bands.For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.
I am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research. Can someone let me know if I’m getting the right HIF band or just some non-specific bands? Thanks.
- (1) HIF-1 alpha’s theoretical molecular weight is 93kDa. The post translationally modified/ubiquitinated form of HIF-1 alpha protein (fails to undergo proteasomal degradation) shows up as a band in the 110-130 kDa range on a Western blot.(2) The dimeric protein may appear at a position above 200 kDa on non-reducing gels.(3) Importantly, HIFs are among the most rapidly degradable proteins; therefore, sample preparation is highly important when analyzing HIF1 alpha or HIF2 alpha. When degraded, HIF-1 alpha may show up between 40-80 kDa position on Western blot. Degradation may be avoided by preparing the samples as soon as possible after collection of cells/tissues in hypoxic chamber. Notably, the tissues/cells should be kept on ice during lysate preparation and the lysates should be analyzed as soon as possible.(4) For troubleshooting suggestions/feedback on more than 25 similar frequently asked questions, I would recommend visiting Novus page: FAQs - Hypoxia and HIFs (5) Last but not the least, Novus technical support team may be contacted via email
I have Hif1a nuclear protein extract at -80C. I am wondering if anyone knows how long it would be good for at that temperature since HIf1a is known to be degraded easily.Thank you!
- You could try a few things to further inhibit the degradation.1) Use the protease inhibitors (if you are not already using them).2) Lyse cells into a buffer that contains SDS or LDS (eg: Laemmli's buffer), since SDS and LDS denature and inhibit proteases. Lysis may even be performed with reducing agents in the buffer (eg. DTT), but this will make your lysates unsuitable for BCA assay.3) Lysing samples rapidly ensures that the samples are instantly homogenized (it also shears DNA released by the SDS).5) Flash-freezing samples in liquid nitrogen rather than freezing at -80*C reduces the window of time for protease activity.6) Freeze samples in individual aliquots, instead of thawing the same vial multiple times.
I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?
- CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.
I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?
- CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.
Is cross-reactivity with HIF-2 alpha tested/predicted?
- Although we don’t have cross-reactivity data with regards to HIF-2 alpha, we predict minimal cross-reactivity based on low sequence similarity observed from BLAST analysis between HIF-1 alpha and HIF-2 alpha.
What is the difference between NB100-105 and NB100-123?
- NB100-105 and NB100-123 are similar as far as immunogens and clones are concerned. However, they are different with respect to their purification process, concentrations and recommended dilutions. NB100-123 is Protein A purified IgG2b with a concentration of 1.5 mg/ml, whereas NB100-105 is Protein G purified IgG2b with 4.0 mg/ml concentration. Another difference is the recommended dilutions (because of the differences in their concentration).
NB100-105 has the same clone name (H1alpha67) as NB100-123.Are these the same clone?
- These are the same clones, however their purifications are slightly different (and thus their recommended dilutions for certain applications are a bit different, please see the section "Applications/Dilutions" on the specific product pages for differences).NB100-123: Protein A purifiedThe basis for purification of IgG, IgG fragments and IgG subclasses is the high affinity of protein A and protein G for the Fc region of polyclonal and monoclonal IgG-type antibodies. Protein A and protein G are bacterial proteins, which, when coupled to chromatography matrix such as Agarose or Sepharose, generate exceptionally useful, easy to use media (resin) for many routine applications. Protein A/G is a recombinant of Protein A and Protein G that has the additive binding properties of both proteins. Protein A, protein G and protein A/G can be used for purification of monoclonal IgG-type antibodies, purification of polyclonal IgG subclasses, and the adsorption and purification of immune complexes involving IgG. IgG subclasses can be isolated from cell culture supernatants and serum and from ascites fluid.Lastly, we have been producing NB100-105 for a longer time period, and thus why it has considerably more publication references than NB100-123.

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Control Lysate(s)

Human Lung Whole Tissue Lysate (Adult Whole Normal)
COS-7 Nuclear Hypoxic Induced Cell Lysate
HeLa CoCl2 treated/Untreated Cell Lysate
HepG2 CoCl2 treated/Untreated Cell Lysate
HeLa Hypoxic / Normoxic Cell Lysate
HepG2 Hypoxic / Normoxic Cell Lysate
HIF-1 alpha Knockout CoCl2-treated/untreated HeLa Cell Lysate
HIF-1 alpha Knockout Hypoxic-treated/untreated HeLa Cell Lysate
NIH 3T3 CoCl2 treated/Untreated Cell Lysate
L929 CoCl2 treated/Untreated Cell Lysate
Raw 264.7 CoCl2 treated/Untreated Cell Lysate
PC12 CoCl2 treated/Untreated Cell Lysate
PC12 Hypoxic / Normoxic Cell Lysate
Raw 264.7 Hypoxic / Normoxic Cell Lysate
L929 Hypoxic / Normoxic Cell Lysate
NIH 3T3 Hypoxic / Normoxic Cell Lysate

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Blogs on HIF-1 alpha. Showing 1-10 of 37 blog posts - Show all blog posts.

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Hypoxia-Dependent CAR Stabilizing Construct in T cells Improves Solid Tumor Targeting and Efficacy
By Victoria Osinski, PhDDespite advances in the development of cancer immunotherapies, those specifically targeting tumors still remains limited. Currently, there is great interest in utilizing chimeric antigen rece... Read full blog post.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ... Read full blog post.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff... Read full blog post.

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Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax... Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ... Read full blog post.

Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling
By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric ... Read full blog post.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy... Read full blog post.

Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor
By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve... Read full blog post.

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HAF007

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Species:	Human

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HIF1A

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

HIF-1 alpha Antibody (H1alpha67)

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Reviews for HIF-1 alpha Antibody (NB100-123) (9) 4.29

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