I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?

Name: HE4/WFDC2 Antibody (OTI1E12) [CoraFluor&#8482; 1]
Brand: Novus Biologicals
SKU: NBP2-71599CL1
Price: 1069 USD

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At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following here

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, IHC
Clone	OTI1E12
Clonality	Monoclonal
Host	Mouse
Conjugate	CoraFluor 1

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP2-71599AF350

Alexa Fluor 405 NBP2-71599AF405

Alexa Fluor 488 NBP2-71599AF488

Alexa Fluor 532 NBP2-71599AF532

Alexa Fluor 594 NBP2-71599AF594

Alexa Fluor 647 NBP2-71599AF647

Alexa Fluor 700 NBP2-71599AF700

Alexa Fluor 750 NBP2-71599AF750

Allophycocyanin NBP2-71599APC

Biotin NBP2-71599B

DyLight 350 NBP2-71599UV

DyLight 405 NBP2-71599V

DyLight 488 NBP2-71599G

DyLight 550 NBP2-71599R

DyLight 594 NBP2-71599DL594

DyLight 650 NBP2-71599C

DyLight 680 NBP2-71599FR

DyLight 755 NBP2-71599IR

FITC NBP2-71599F

HRP NBP2-71599H

Janelia Fluor 525 NBP2-71599JF525

Janelia Fluor 549 NBP2-71599JF549

Janelia Fluor 646 NBP2-71599JF646

Janelia Fluor 669 NBP2-71599JF669

PE NBP2-71599PE

PerCP NBP2-71599PCP

Unconjugated NBP2-46360

Catalog# & Formulation	Size	Price
Standard Formulation NBP2-46360

Azide and BSA Free NBP2-71599

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-71599AF350
Alexa Fluor 405	NBP2-71599AF405
Alexa Fluor 488	NBP2-71599AF488
Alexa Fluor 532	NBP2-71599AF532
Alexa Fluor 594	NBP2-71599AF594
Alexa Fluor 647	NBP2-71599AF647
Alexa Fluor 700	NBP2-71599AF700
Alexa Fluor 750	NBP2-71599AF750
Allophycocyanin	NBP2-71599APC
Biotin	NBP2-71599B
DyLight 350	NBP2-71599UV
DyLight 405	NBP2-71599V
DyLight 488	NBP2-71599G
DyLight 550	NBP2-71599R
DyLight 594	NBP2-71599DL594
DyLight 650	NBP2-71599C
DyLight 680	NBP2-71599FR
DyLight 755	NBP2-71599IR
FITC	NBP2-71599F
HRP	NBP2-71599H
Janelia Fluor 525	NBP2-71599JF525
Janelia Fluor 549	NBP2-71599JF549
Janelia Fluor 646	NBP2-71599JF646
Janelia Fluor 669	NBP2-71599JF669
PE	NBP2-71599PE
PerCP	NBP2-71599PCP
Unconjugated	NBP2-46360

HE4/WFDC2 Antibody (OTI1E12) [CoraFluor™ 1]

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Description	CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen	Human recombinant protein fragment corresponding to amino acids 31-124 of human WFDC2 (NP_006094) produced in E.coli.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	WFDC2
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Immunohistochemistry Immunohistochemistry-Paraffin Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark. Do not freeze.
Buffer	PBS
Preservative	No Preservative
Purity	Immunogen affinity purified