![Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp75 antibody diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Western-Blot-NBP1-31596-img0007.jpg "Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp75 antibody diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GRP75/HSPA9B/Mortalin. The exact sequence is proprietary. |
| Localization | Mitochondrion |
| Marker | Immortalization Marker |
| Predicted Species | Xenopus (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HSPA9 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 74 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for GRP75/HSPA9B/Mortalin Antibody (NBP1-31596)Find related products by research area.
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| Gene Symbol | HSPA9 |
![Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Grp75 stained by Grp75 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31596-img0002.jpg "Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Grp75 stained by Grp75 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Rat duodenum. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunohistochemistry-Paraffin-NBP1-31596-img0005.jpg "Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Rat duodenum. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Whole cell extract (6 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Western-Blot-NBP1-31596-img0003.jpg "Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Whole cell extract (6 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Rat brain. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunohistochemistry-Paraffin-NBP1-31596-img0001.jpg "Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Rat brain. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Mouse duodenum. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunohistochemistry-Paraffin-NBP1-31596-img0004.jpg "Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Mouse duodenum. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunoprecipitation: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Immunoprecipitation of GRP75 protein from HepG2 whole cell extracts using 5 ug of GRP75 antibody. Western blot analysis was performed using GRP75 antibody diluted at 1:2500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunoprecipitation-NBP1-31596-img0008.jpg "Immunoprecipitation: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Immunoprecipitation of GRP75 protein from HepG2 whole cell extracts using 5 ug of GRP75 antibody. Western blot analysis was performed using GRP75 antibody diluted at 1:2500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31596_rabbit-polyclonal-grp75-hspa9b-mortalin-antibody-91020242041215.jpg "Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.GRP75/HSPA9B/Mortalin stained by GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31596_rabbit-polyclonal-grp75-hspa9b-mortalin-antibody-91020242075965.jpg "Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.GRP75/HSPA9B/Mortalin stained by GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.GRP75/HSPA9B/Mortalin stained by GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31596_rabbit-polyclonal-grp75-hspa9b-mortalin-antibody-910202420115040.jpg "Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.GRP75/HSPA9B/Mortalin stained by GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![Bioactivity GM-CSF [Unconjugated]](https://images.novusbio.com/images/protein/7954-gmcf_recombinant-human-gm-csf-cho-expressed-protein-cf-bioactivity-272020121514.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![SDS-PAGE IL-3 [Unconjugated]](https://images.novusbio.com/images2/IL-3_203-IL_345.jpg)
![Bioactivity IL-3 [Unconjugated]](https://images.novusbio.com/images2/IL-3_203-IL_347.jpg)
![SEC-MALS IL-3 [Unconjugated]](https://images.novusbio.com/images/203-il_recombinant-human-il-3-protein-sec-mals-133202482656..jpg)
![Neutralization G-CSF Antibody (67604) [Unconjugated]](https://images.novusbio.com/images2/G-CSF_MAB414_Block_Neutralize_9188.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")