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GRK1 Antibody (D11) [DyLight 488]

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Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
D11
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB120-2776AF350
Alexa Fluor 405
NB120-2776AF405
Alexa Fluor 488
NB120-2776AF488
Alexa Fluor 532
NB120-2776AF532
Alexa Fluor 594
NB120-2776AF594
Alexa Fluor 647
NB120-2776AF647
Alexa Fluor 700
NB120-2776AF700
Alexa Fluor 750
NB120-2776AF750
Biotin
NB120-2776B
DyLight 350
NB120-2776UV
DyLight 405
NB120-2776V
DyLight 550
NB120-2776R
DyLight 594
NB120-2776DL594
DyLight 650
NB120-2776C
DyLight 680
NB120-2776FR
DyLight 755
NB120-2776IR
FITC
NB120-2776F
HRP
NB120-2776H
Janelia Fluor 549
NB120-2776JF549
Janelia Fluor 646
NB120-2776JF646
Unconjugated
NB120-2776
mFluor Violet 450 SE
NB120-2776MFV450
mFluor Violet 500 SE
NB120-2776MFV500
mFluor Violet 610 SE
NB120-2776MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB120-2776
BSA Free
NB120-2776
BSA Free
NB120-2776-0.025mg
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GRK1 Antibody (D11) [DyLight 488] Summary

Immunogen
Full length human GRK1.
Epitope
This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.
Specificity
Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This does not cross-react with other G protein-coupled kinases.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GRK1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for GRK1 Antibody (D11) [DyLight 488]

  • G protein-coupled receptor kinase 1rhodopsin kinase
  • GPRK1
  • GRK1
  • RHOK
  • RHOKEC 2.7.11
  • RKEC 2.7.11.14

Background

The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GRK1 Antibody
(D11) [DyLight 488]
NB120-2776G
GRK1 Antibody (D11) [DyLight 488]
Species: Hu, Bv
Applications: WB, ICC/IF, IHC, IP

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GRK1