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GRB2 Antibody

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Western Blot: GRB2 Antibody [NBP2-55208] - Analysis using Anti-GRB2 antibody NBP2-55208 (A) shows similar pattern to independent antibody NBP2-55209 (B).
Independent Antibodies: Western Blot: GRB2 Antibody [NBP2-55208] - Analysis using Anti-GRB2 antibody NBP2-55208 (A) shows similar pattern to independent antibody NBP2-55209 (B).
Immunocytochemistry/ Immunofluorescence: GRB2 Antibody [NBP2-55208] - Staining of human cell line PC-3 shows localization to nucleus & nucleoli.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

        

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GRB2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: KVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPT
Predicted Species
Mouse (98%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRB2
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
GRB2 Recombinant Protein Antigen (NBP2-55208PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for GRB2 Antibody

  • abundant SRC homology
  • Adapter protein GRB2
  • ASH
  • EGFRBP-GRB2
  • epidermal growth factor receptor-binding protein GRB2
  • GRB2
  • Grb3-3
  • growth factor receptor-bound protein 2
  • growth factor receptor-bound protein 3
  • HT027
  • MST084
  • MSTP084
  • NCKAP2
  • Protein Ash
  • SH2/SH3 adapter GRB2

Background

The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GRB2