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Glut2 Antibody (205115) [Unconjugated]

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Glut2 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Rat Anti-Mouse Glut2 Monoclonal Antibody (Catalog # MAB1440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Glut2 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Rat Anti-Mouse Glut2 Monoclonal Antibody (Catalog # MAB1440) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
beta TC-6 mouse beta cell insulinoma cell line was stained with Rat Anti-Mouse Glut2 Monoclonal Antibody (Catalog # MAB1440, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
205115
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1440U-100UG
Alexa Fluor 405
FAB1440V-100UG
Alexa Fluor 488
FAB1440G-100UG
Alexa Fluor 532
FAB1440X
Alexa Fluor 594
FAB1440T-100UG
Alexa Fluor 647
FAB1440R-100UG
Alexa Fluor 700
FAB1440N-100UG
Alexa Fluor 750
FAB1440S-100UG
Allophycocyanin
FAB1440A-100UG
Allophycocyanin/Cy7
FAB1440APCCY7
Biotin
BAM1440
DyLight 350
FAB1440D
DyLight 405
FAB1440E
DyLight 488
FAB1440K
DyLight 550
FAB1440L
DyLight 594
FAB1440M
DyLight 650
FAB1440W
DyLight 680
FAB1440Y
DyLight 755
FAB1440Z
FITC
FAB1440F
HRP
FAB1440H
Janelia Fluor 525
FAB1440JF525
Janelia Fluor 549
FAB1440I
Janelia Fluor 646
FAB1440J
Janelia Fluor 669
FAB1440JF669
PE/Atto594
FAB1440PEATT594
PE/Cy5.5
FAB1440PECY55
PE/Cy7
FAB1440PECY7
PerCP
FAB1440C
Phycoerythrin
FAB1440P-100UG
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1440-SP
Carrier Free
FAB1440V-100UG
Carrier Free
FAB1440U-100UG
Carrier Free
FAB1440T-100UG
Carrier Free
FAB1440S-100UG
Carrier Free
FAB1440R-100UG
Carrier Free
FAB1440N-100UG
Carrier Free
FAB1440G-100UG
Carrier Free
FAB1440A-100UG
Carrier Free
FAB1440P-100UG
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Glut2 Antibody (205115) [Unconjugated] Summary

Immunogen
CHO Chinese hamster ovary cell line transfected with mouse Glut2
Met1-Val523
Accession # P14246
Specificity
Detects mouse Glut2. Recognizes mouse Glut2-transfected NS0 cells but not NS0 control transfectants. It also detects Glut2 on mouse insulinoma beta TC-6 cells (2).
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
SLC2A2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publication using
MAB1440 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glut2 Antibody (205115) [Unconjugated]

  • GLUT2 Glucose transporter type 2, liver
  • Glut2
  • GLUT-2
  • SLC2A2
  • solute carrier family 2 (facilitated glucose transporter), member 2
  • solute carrier family 2, facilitated glucose transporter member 2

Background

Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state (1).

  1. Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
  2. Poitout, V. et al. (1995) Diabetes 44:306.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut2 Antibody (MAB1440)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SLC2A2
Uniprot