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Glucagon Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Analysis in human pancreas and skeletal muscle tissues. Corresponding GCG RNA-seq data are presented for the same tissues.
Independent Antibodies: Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human colon, pancreas, skeletal muscle and small intestine using Anti-GCG antibody NBP2-38329 (A) shows similar ...read more
Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP2-38329] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human pancreas shows strong cytoplasmic positivity in endocrine glandular cells.
Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.
Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human colon shows moderate cytoplasmic positivity in enteroendocrine cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Glucagon Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GCG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
Glucagon Recombinant Protein Antigen (NBP2-38329PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Glucagon Antibody

  • GCG
  • glicentin-related polypeptide
  • GLP1
  • GLP-1
  • Glucagon
  • glucagon-like peptide 1
  • glucagon-like peptide 2
  • GRPP

Background

The protein encoded by the Glucagon gene is actually a preproprotein that is cleaved into four distinct mature peptides. One ofthese, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulatingglycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signallingpathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promotenutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an activeenteroglucagon. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GCG
Uniprot