Immunocytochemistry/ Immunofluorescence: Glucagon Antibody (09) [NBP2-21803] - Staining in human pancreas tissueSignal is strong in alpha cells. Dilution: 1:4000
Immunohistochemistry-Paraffin: Glucagon Antibody (09) [NBP2-21803] - Staining of human pancreas tissue . Alpha cells of the Langerhans islet were stained strongly. Dilution: 1:8000
Sandwich ELISA: Glucagon Antibody (09) - BSA Free [NBP2-21803] - Glucagon Antibody (09) [NBP2-21803] - The calibration curve of a sandwich assay for Glucagon using NBP2-21803 as the capture antibody and NBP2-21802B as ...read more
Use in Flow reported in scientific literature (PMID:33284818). ELISA: NBP2-21803 binds free glucagon in solution. NBP2-21803 when used as capture antibody forms a sandwich ELISA pair with NBP2-21802B as biotinylated detection antibody for measuring glucagon. IHC: NBP2-21803 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:8000. IF: NBP2-21803 was used in IF with a dilution of 1:4000.
Publications
Read Publications using NBP2-21803 in the following applications:
Use in Mouse reported in scientific literature (PMID:32953935).
Packaging, Storage & Formulations
Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
0.9% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified
Alternate Names for Glucagon Antibody (09) - BSA Free
GCG
glicentin-related polypeptide
GLP1
GLP-1
Glucagon
glucagon-like peptide 1
glucagon-like peptide 2
GRPP
Background
The protein encoded by the Glucagon gene is actually a preproprotein that is cleaved into four distinct mature peptides. One ofthese, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulatingglycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signallingpathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promotenutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an activeenteroglucagon. (provided by RefSeq)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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