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Glucagon Antibody (09) - BSA Free

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Immunocytochemistry/ Immunofluorescence: Glucagon Antibody (09) [NBP2-21803] - Staining in human pancreas tissueSignal is strong in alpha cells. Dilution: 1:4000
Immunohistochemistry-Paraffin: Glucagon Antibody (09) [NBP2-21803] - Staining of human pancreas tissue . Alpha cells of the Langerhans islet were stained strongly. Dilution: 1:8000
Sandwich ELISA: Glucagon Antibody (09) - BSA Free [NBP2-21803] - Glucagon Antibody (09) [NBP2-21803] - The calibration curve of a sandwich assay for Glucagon using NBP2-21803 as the capture antibody and NBP2-21802B as ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, ELISA
Clone
09
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Glucagon Antibody (09) - BSA Free Summary

Immunogen
Synthetic full length oxyntomodulin coupled to carrier.
Epitope
The epitope lies within the C-terminal region of glucagon.
Specificity
Binds human glucagon. Cross-reacts less than 5% with oxyntomodulin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GCG
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:4000
  • Immunohistochemistry 1:8000
  • Immunohistochemistry-Paraffin 1:8000
  • Sandwich ELISA Capture
  • Sandwich ELISA 1:100-1:2000
Application Notes
Use in Flow reported in scientific literature (PMID:33284818). ELISA: NBP2-21803 binds free glucagon in solution. NBP2-21803 when used as capture antibody forms a sandwich ELISA pair with NBP2-21802B as biotinylated detection antibody for measuring glucagon. IHC: NBP2-21803 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:8000. IF: NBP2-21803 was used in IF with a dilution of 1:4000.
Publications
Read Publications using
NBP2-21803 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32953935).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
0.9% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Glucagon Antibody (09) - BSA Free

  • GCG
  • glicentin-related polypeptide
  • GLP1
  • GLP-1
  • Glucagon
  • glucagon-like peptide 1
  • glucagon-like peptide 2
  • GRPP

Background

The protein encoded by the Glucagon gene is actually a preproprotein that is cleaved into four distinct mature peptides. One ofthese, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulatingglycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signallingpathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promotenutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an activeenteroglucagon. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glucagon Antibody (NBP2-21803)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 4 applications: FLOW, ICC/IF, IF/IHC, WB.


Filter By Application
FLOW
(1)
ICC/IF
(1)
IF/IHC
(1)
WB
(1)
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Human
(1)
Mouse
(1)
Porcine
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Showing Publications 1 - 7 of 7.
Publications using NBP2-21803 Applications Species
Wu Y, Wong CW, Chiles EN Et al. Glycerate from intestinal fructose metabolism induces islet cell damage and glucose intolerance Cell Metab 2022-06-10 [PMID: 35688154]

Details:
Citation using the Alexa Fluor 647 version of this antibody.
Xi Y, Song B, Ngan I et al. Glucagon-receptor-antagonism-mediated beta-cell regeneration as an effective anti-diabetic therapy Cell reports 2022-05-31 [PMID: 35649369] (ICC/IF) ICC/IF
W Tang, Y Zhong, Y Wei, Z Deng, J Mao, J Liu, TG Valencak, J Liu, H Xu, H Wang Ileum tissue single-cell mRNA sequencing elucidates the cellular architecture of pathophysiological changes associated with weaning in piglets Bmc Biology, 2022-05-30;20(1):123. 2022-05-30 [PMID: 35637473]
Lau H, Corrales N, Rodriguez S et al. Dose-dependent effects of necrostatin-1 supplementation to tissue culture media of young porcine islets PloS one 2020-12-07 [PMID: 33284818] (FLOW, Porcine) FLOW Porcine
Zhao J, Yue Y, Patel A et al. High-Resolution Histological Landscape of AAV DNA Distribution in Cellular Compartments and Tissues following Local and Systemic Injection Molecular Therapy - Methods & Clinical Development 2020-09-01 [PMID: 32953935] (IF/IHC, Mouse) IF/IHC Mouse
Lau, H, Corrales, N Et al. An islet maturation media to improve the development of young porcine islets during in vitro culture. Islets 2020-05-03 [PMID: 32459554] (WB, Human) WB Human
Lau H, Corrales N, Alexander M et al. Necrostatin-1 supplementation enhances young porcine islet maturation and in vitro function Xenotransplantation [PMID: 31532037]

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GCG