Rat GLP1 ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Rat GLP1 in samples. No significant cross-reactivity or interference between Rat GLP1 and analogues was observed. |
Standard Curve Range |
1.56-100 pg/mL |
Sensitivity |
0.94 pg/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
%CV < 5.45 |
Intra-Assay |
%CV < 5.49 |
Spike Recovery |
83-103% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
GCG |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Rat GLP1 ELISA Kit (Colorimetric)
Background
Glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted from enteroendocrine L cells in response to ingested nutrients. The closely related peptides glucagon-like peptide (GLP-1) and glucagon have opposing effects on blood glucose. GLP-1 induces glucose-dependent insulin secretion in the pancreas, while glucagon stimulates gluconeogenesis and glycogenolysis in the liver. Glucagon is processed from a large precursor, proglucagon, in a tissue-specific manner in pancreatic alpha-cells. The identification of a hybrid peptide acting as both a GLP-1 agonist and a glucagon antagonist would provide a novel approach for the treatment of type 2 diabetes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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