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Mouse Ghrelin ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Standard Curve Range
123.5 - 10000 pg/mL
Sensitivity
45.6 pg/mL

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Mouse Ghrelin ELISA Kit (Colorimetric) Summary

Description
Assay time: 2 hours
Standard Curve Range
123.5 - 10000 pg/mL
Sensitivity
45.6 pg/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GHRL

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30x concentrate)

Alternate Names for Mouse Ghrelin ELISA Kit (Colorimetric)

  • appetite-regulating hormone
  • Ghrelin
  • ghrelin/obestatin preprohormone
  • ghrelin/obestatin prepropeptide
  • GHRL
  • Growth hormone secretagogue
  • Growth hormone-releasing peptide
  • Motilin-related Peptide
  • MTLRPgrowth hormone secretagogue receptor ligand
  • obestatin
  • Protein M46

Background

Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Mouse Ghrelin
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Product General Protocols

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As the hormone ghrelin is linked to appetite and weight gain, as well as impaired glucose-induced insulin secretion, there is considerable interest in this peptide as a potential drug target. Although the overall lack of success in this field has been...  Read full blog post.
Shades of Ghrelin in Weight Homeostasis
The neuropeptide and gut hormone Ghrelin is an endogenous ligand for the growth hormone (GH)-secretagogue receptor (GHSR) within the central nervous system. This pathway has received a great deal of attention and heavy study within the last decade bec...  Read full blog post.
Appetite and Energy: A Ghrelin Balancing Act
Ghrelin is the only potent orexigenic peptide in circulation. It stimulates food intake and leads to metabolism regulation, positive energy balance, adipogenesis, and body weight gain. However, in studies using ghrelin antibodies, the physiological si...  Read full blog post.
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Bioinformatics

Gene Symbol GHRL