![Western Blot of GFP antibody [FITC]. Lane 1: GFP. Lane 2: None. Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT.Block for 30 min at RT.Predicted/Observed size: 28 kDa, 33 kDa for GFP. Other band(s): None.](http://images.novusbio.com/fullsize/GFP-Antibody-[FITC]-Western-Blot-NB100-1771-img0013.jpg "Western Blot of GFP antibody [FITC]. Lane 1: GFP. Lane 2: None. Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT.Block for 30 min at RT.Predicted/Observed size: 28 kDa, 33 kDa for GFP. Other band(s): None.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, DB, ELISA, Flow, Func, ICC/IF, IHC, KD, KO |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | FITC |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB600-1502 | |||
| Biotin | NB100-1678 | |||
| DyLight 488 | NBP1-69969 | |||
| FITC | NB100-1771SS | |||
| HRP | NB600-313 | |||
| Rhodamine | NBP1-97327-500ug | |||
| Texas Red | NB120-6660 | |||
| Unconjugated | NB100-1770 | |||
| Description | For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room GFP Fluorescein Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria) |
| Immunogen | The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. (Uniprot: P42212) |
| Specificity | No reaction was observed against Human, Mouse and Rat Serum Proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. Primary antibody: GFP antibody at 1 ug/ml for 1 h at room temperature. Secondary antibody: IRDye 800 conjugated Donkey-a-Goat IgG [H&L] MX7 secondary antibody at 1:2,500 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 33 kDa for GFP. Other band(s): none.](http://images.novusbio.com/fullsize/GFP-Antibody-[FITC]-Western-Blot-NB100-1771-img0011.jpg "Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. Primary antibody: GFP antibody at 1 ug/ml for 1 h at room temperature. Secondary antibody: IRDye 800 conjugated Donkey-a-Goat IgG [H&L] MX7 secondary antibody at 1:2,500 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 33 kDa for GFP. Other band(s): none.")
