![Immunocytochemistry/Immunofluorescence: GCH1 Antibody (2C4) [H00002643-M02] - Analysis of monoclonal antibody to GCH1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/GCH1-Antibody-2C4-Immunocytochemistry-Immunofluorescence-H00002643-M02-img0004.jpg "Immunocytochemistry/Immunofluorescence: GCH1 Antibody (2C4) [H00002643-M02] - Analysis of monoclonal antibody to GCH1 on HeLa cell . Antibody concentration 10 ug/ml.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, ICC/IF |
| Clone | 2C4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | GCH1 (AAH25415.1, 1 a.a. ~ 250 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEKGPVRAPAEKPRGARCSNGFPERDPPRPGPSRPAEKPPRPEAKSAQPADGWKGERPRSEEDNELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATHMCMVMRGVQKMNSKTVTSTMLGVFREDPKTREEFLTLIRS |
| Specificity | GCH1 - GTP cyclohydrolase 1 (dopa-responsive dystonia) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GCH1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control. It has been used for IF. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
![Simple Western GCHFR Antibody (846011) [Unconjugated]](https://images.novusbio.com/images2/GCHFR_MAB7918_Simple_Western_16530.jpg)
![Western Blot GCHFR Antibody (846011) [Unconjugated]](https://images.novusbio.com/images2/GCHFR_MAB7918_Western_Blot_13066.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Sandwich ELISA: GCH1 Antibody (2C4) [H00002643-M02] - Detection limit for recombinant GST tagged GCH1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GCH1-Antibody-2C4-Sandwich-ELISA-H00002643-M02-img0005.jpg "Sandwich ELISA: GCH1 Antibody (2C4) [H00002643-M02] - Detection limit for recombinant GST tagged GCH1 is approximately 0.1ng/ml as a capture antibody.")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot Dopa Decarboxylase/DDC Antibody [Unconjugated]](https://images.novusbio.com/images2/Dopa_Decarboxylase_AF3564_Western_Blot_17092.jpg)
![Immunohistochemistry Dopa Decarboxylase/DDC Antibody [Unconjugated]](https://images.novusbio.com/images2/Dopa_Decarboxylase_AF3564_Immunohistochemistry_19241.jpg)
![Immunohistochemistry Dopa Decarboxylase/DDC Antibody [Unconjugated]](https://images.novusbio.com/images2/Dopa_Decarboxylase_AF3564_Immunohistochemistry_19242.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")