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GATA-1 Antibody (234732) [Phycoerythrin]

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MG-63 human osteosarcoma cell line was stained with Rat Anti-Human GATA-1 PE-conjugated Monoclonal Antibody (Catalog # IC1779P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
MG-63 human osteosarcoma cell line was stained with Rat Anti-Human GATA-1 PE-conjugated Monoclonal Antibody (Catalog # IC1779P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
234732
Clonality
Monoclonal
Host
Rat
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
MAB1779
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1779
Carrier Free
MAB1779
Carrier Free
MAB1779-SP
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GATA-1 Antibody (234732) [Phycoerythrin] Summary

Immunogen
E. coli-derived recombinant human GATA-1
Met1-Ser413
Accession # P15976
Specificity
Detects human GATA-1 in direct ELISAs. In Western blots, this antibody does not cross-react with recombinant human (rh) GATA-2 or rhGATA-6.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
GATA1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-1 Antibody (234732) [Phycoerythrin]

  • Eryf1
  • ERYF1GATA-binding protein 1 (globin transcription factor 1)
  • GATA binding protein 1 (globin transcription factor 1)
  • GATA1
  • GATA-1
  • GATA-1erythroid transcription factor
  • GATA-binding factor 1
  • GF-1
  • GF1globin transcription factor 1
  • NF-E1 DNA-binding protein
  • NFE1erythroid transcription factor 1
  • transcription factor GATA1
  • XLTT

Background

GATA-1 is the founding member of the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue‑specific programs of gene expression and morphogenesis. GATA-1 is expressed in blood forming cells. It interacts with several additional proteins to activate or repress gene expression and is essential for erythropoiesis (1).

  1. Patient, R.K. and J. McGhee (2002) Curr. Opin. Genet. Dev. 12:416.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GATA1
Uniprot