Western Blot: Galanin Antibody [NBP1-45217] - Staining of Mouse (A) and Rat (B) Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western Blot: Galanin Antibody [NBP1-45217] - Analysis of Galanin in Human Cerebellum lysate (35ug protein in RIPA buffer) using NBP1-45217 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
WB: Approx. 17 kDa band observed in human brain (cerebellum) and in mouse and rat brain lysates (calculated MW of 13.3 kDa band according to human NP_057057.2 and of 13.5 kDa band according to mouse NP_034383.1 and rat NP_150240.1).
Publications
Read Publications using NBP1-45217 in the following applications:
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified
Alternate Names for Galanin Antibody
GAL
GAL1
galanin prepropeptide
Galanin
GALN
GALNgalanin-related peptide
GLNNgalanin-message-associated peptide
GMAP
MGC40167
Background
Galanin is a 29/30 amino acid neuropeptide widely distributed in the peripheral and central nervous systems and endocrine system. The amino acid sequence of galanin is very conserved (almost 90% among species), indicating the importance of the molecule. In the brain, the highest concentrations are observed in the hypothalamus and particularly in the nerve terminals of the median eminence. There are three well-characterized G-protein coupled galanin receptors subtypes (GalR1-3). A more recently discovered 60 amino acid galanin-like peptide (GALP) shares amino acid sequence homology with galanin (1-13) in position 9-21 and has high binding affinity for GalR1-3, with highest affinity for GalR3. The fact that Galanin has a wide spectrum of activities indicates that it is an important messenger for intercellular communication within the nervous system and the neuroendocrine axis. Both galanin and GALP are implicated in the regulation of food and water intake behavior, and many related neuroendocrine functions.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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