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Human GAD1/GAD67 ELISA Kit (Colorimetric)

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ELISA: Human GAD1/GAD67 ELISA Kit (Colorimetric) [NBP2-67965] - Samples were spiked with high concentrations of Human GAD1/GAD67 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human GAD1/GAD67 ELISA Kit (Colorimetric) [NBP2-67965] - Samples were spiked with high concentrations of Human GAD1/GAD67 and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Human GAD1/GAD67 ELISA Kit (Colorimetric) [NBP2-67965] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL

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Human GAD1/GAD67 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human GAD1 in samples. No significant cross-reactivity or interference between Human GAD1 and analogues was observed.
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.61%
Intra-Assay
CV% < 5.22%
Spike Recovery
84-110%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GAD1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human GAD1/GAD67 ELISA Kit (Colorimetric)

  • 67kD)
  • EC 4.1.1
  • FLJ45882
  • GAD1
  • GAD25
  • GAD67
  • glutamate decarboxylase 1 (brain, 67kDa)
  • Glutamate decarboxylase 67 kDa isoform

Background

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Human GAD1/GAD67 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol GAD1