![Western Blot: GAD1/GAD67 Antibody (GAD1/2391) [NBP3-08276] - Western Blot Analysis of human Brain tissue lysate using GAD1/GAD67 Mouse Monoclonal Antibody (GAD1/2391).](http://images.novusbio.com/fullsize/GAD1-GAD67-Antibody-GAD1-2391-Western-Blot-NBP3-08276-img0002.jpg "Western Blot: GAD1/GAD67 Antibody (GAD1/2391) [NBP3-08276] - Western Blot Analysis of human Brain tissue lysate using GAD1/GAD67 Mouse Monoclonal Antibody (GAD1/2391).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, MA |
| Clone | GAD1/2391 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07357). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human GAD1/GAD67 protein fragment (around aa 72-135) (exact sequence is proprietary) (Uniprot: Q99259) |
| Localization | Cytoplasmic |
| Marker | GABAergic Neuronal Marker |
| Specificity | This monoclonal antibody recognizes a protein of 67kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GAD1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: For coating, order antibody without BSA |
| Theoretical MW | 67 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for GAD1/GAD67 Antibody (NBP3-08276)Find related products by research area.
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| Gene Symbol | GAD1 |
![Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (GAD1/2391) [NBP3-08276] - Formalin-fixed, paraffin-embedded human Brain stained with GAD1/GAD67 Mouse Monoclonal Antibody (GAD1/2391).](http://images.novusbio.com/fullsize/GAD1-GAD67-Antibody-GAD1-2391-Immunohistochemistry-Paraffin-NBP3-08276-img0001.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (GAD1/2391) [NBP3-08276] - Formalin-fixed, paraffin-embedded human Brain stained with GAD1/GAD67 Mouse Monoclonal Antibody (GAD1/2391).")
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