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GAD1/GAD67 Antibody (CL2911)

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Western Blot: GAD1/GAD67 Antibody (2911) [NBP2-46637] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Western Blot: GAD1/GAD67 Antibody (2911) [NBP2-46637] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (2911) [NBP2-46637] - Staining of human pancreas shows absence of immunoreactivity (negative control).
Immunohistochemistry: GAD1/GAD67 Antibody (2911) [NBP2-46637] - Staining of rat globus pallidus shows strong immunoreactivity in GABAergic neural fibers.
Immunohistochemistry: GAD1/GAD67 Antibody (2911) [NBP2-46637] - Staining of mouse substantia nigra shows strong immunoreactivity in GABAergic neural processes.
Immunohistochemistry: GAD1/GAD67 Antibody (2911) [NBP2-46637] - Staining of rat cerebral cortex shows strong positivity in GABAergic synapses on pyramidal neurons.
Immunohistochemistry: GAD1/GAD67 Antibody (2911) [NBP2-46637] - Staining of mouse cerebellum shows positivity in GABAergic neural fibers.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (2911) [NBP2-46637] - Staining of human cerebral cortex shows immunoreactivity in neuropil, as well as some neuronal cells bodies.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (2911) [NBP2-46637] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells, as well as in fibers in granular and molecular layers.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
CL2911
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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GAD1/GAD67 Antibody (CL2911) Summary

Immunogen
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence QSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRS
Epitope
TETDFSNLFA
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GAD1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
GAD1/GAD67 Recombinant Protein Antigen (NBP2-46637PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for GAD1/GAD67 Antibody (CL2911)

  • 67kD)
  • EC 4.1.1
  • FLJ45882
  • GAD1
  • GAD25
  • GAD67
  • glutamate decarboxylase 1 (brain, 67kDa)
  • Glutamate decarboxylase 67 kDa isoform

Background

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GAD1
Entrez
Uniprot