![Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - FoxP3 Antibody (3G3) [Azide Free] [NBP2-33297] - Analysis in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and NBP2-33297PE (right) at 0.1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate, anti CD25 APC conjugate, anti FOXP3 PE conjugate (NBP2-33297PE).](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Azide-Free-Flow-Cytometry-NBP2-33297-img0007.jpg "Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - FoxP3 Antibody (3G3) [Azide Free] [NBP2-33297] - Analysis in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and NBP2-33297PE (right) at 0.1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate, anti CD25 APC conjugate, anti FOXP3 PE conjugate (NBP2-33297PE).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | 3G3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Full-length His-tagged murine FOXP3 was used as the immunogen. The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein (NP_001107849). |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FOXP3 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS containing 0.2% gelatin. Does not contain BSA |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
![Immunohistochemistry CD3 Antibody (17A2) [Unconjugated]](https://images.novusbio.com/images2/CD3_MAB4841_Immunohistochemistry_7033.jpg)
![Immunocytochemistry CD3 Antibody (17A2) [Unconjugated]](https://images.novusbio.com/images2/CD3_MAB4841_Immunocytochemistry__Immunofluorescence_23528.jpg)
![Immunohistochemistry CD3 Antibody (17A2) [Unconjugated]](https://images.novusbio.com/images/antibody/mab4841_mouse-cd3-mab-clone-17a2-immunohistochemistry-88202312119.jpg)
![Flow Cytometry GITR/TNFRSF18 Antibody (110416) [Unconjugated]](https://images.novusbio.com/images/antibody/mab689_human-gitr-tnfrsf18-mab-clone-110416-flow-cytometry-286202414437..jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for FoxP3 Antibody (NBP2-33297)Find related products by research area.
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Meeting Report: 2nd International Antibody Validation Meeting Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde... Read full blog post. |
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FOXP3 Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function. Distinctively, ... Read full blog post. |
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FOXP3 is a Master Regulator of T Regulatory (Treg) Cells FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of ... Read full blog post. |
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FOXP3: Master Regulatory Transcriptional Factor FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom... Read full blog post. |
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| Gene Symbol | FOXP3 |
![Western Blot: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis in 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Azide-Free-Western-Blot-NBP2-33297-img0001.jpg "Western Blot: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis in 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml.")
![Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.5 ug/10^6 cells. Anti-CD25 APC conjugate this antibody, anti-FOXP3 FITC.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Azide-Free-Flow-Cytometry-NBP2-33297-img0011.jpg "Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.5 ug/10^6 cells. Anti-CD25 APC conjugate this antibody, anti-FOXP3 FITC.")
![Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis in 2x10^6 mouse lymph node cells using 0.1 ug of PE conjugated antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Azide-Free-Flow-Cytometry-NBP2-33297-img0004.jpg "Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis in 2x10^6 mouse lymph node cells using 0.1 ug of PE conjugated antibody.")
![Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.1 ug/10^6 cells. Anti-CD25 FITC conjugate this antibody, anti-FOXP3 FITC.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Azide-Free-Flow-Cytometry-NBP2-33297-img0009.jpg "Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.1 ug/10^6 cells. Anti-CD25 FITC conjugate this antibody, anti-FOXP3 FITC.")
![Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody. 1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Azide-Free-Flow-Cytometry-NBP2-33297-img0008.jpg "Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody. 1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate.")
![Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody, 5 ug/10^6 cells. Products used: anti-CD4 PE conjugate, anti-CD25 APC conjugate, anti-FOXP3 FITC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Azide-Free-Flow-Cytometry-NBP2-33297-img0010.jpg "Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody, 5 ug/10^6 cells. Products used: anti-CD4 PE conjugate, anti-CD25 APC conjugate, anti-FOXP3 FITC conjugate.")
![N/A CD25/IL-2R alpha [HRP]](https://images.novusbio.com/images2/IL-2_R_alpha_DR2A00_ELISA_167.jpg)
![N/A CD25/IL-2R alpha [HRP]](https://images.novusbio.com/images2/DATA_CD25_DR2A00_ELISA_800.jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
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![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![N/A IL-17/IL-17A [Biotin]](https://images.novusbio.com/images2/DATA_IL17_DY421_ELISA_2448.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")