![Western Blot: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Total protein from MOLT-4 (lane 1) and Ntera-2 (lane 2) was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/mL of anti-FGF R1. FGF R1 protein was detected at ~150 kDa using an anti-mouse HRP secondary antibody. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/FGFR1-Antibody-M17D10-Azide-and-BSA-Free-Western-Blot-NBP2-80733-img0001.jpg "Western Blot: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Total protein from MOLT-4 (lane 1) and Ntera-2 (lane 2) was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/mL of anti-FGF R1. FGF R1 protein was detected at ~150 kDa using an anti-mouse HRP secondary antibody. Image from the standard format of this antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | M17D10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant human ectodomain of FGFr1b expressed in E. coli from pro23 to his415; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences. |
| Epitope | Epitope is within the sequence pro179 to lys195 in Ig loop II. |
| Localization | Cell Membrane |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FGFR1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is CyTOF ready. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for FGFR1 Antibody (NBP2-80733)Find related products by research area.
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FGFR1 - regulating cell growth and proliferation in development and disease The vertebrate fibroblast growth factor receptor (FGFR) family is an important group of proteins involved in embryonic development and the growth and proliferation of adult cells. Mutations in FGFR proteins can lead to pathologies including bone o... Read full blog post. |
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| Gene Symbol | FGFR1 |
![Immunocytochemistry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Analysis of FFPE tissue section of human liver using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. The hepatocytes depicted an expected and specific membrane-cytoplasmic-nuclear immunostaining of FGF R1 protein. Image from the standard forma](http://images.novusbio.com/fullsize/FGFR1-Antibody-M17D10-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80733-img0003.jpg "Immunocytochemistry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Analysis of FFPE tissue section of human liver using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. The hepatocytes depicted an expected and specific membrane-cytoplasmic-nuclear immunostaining of FGF R1 protein. Image from the standard forma")
![Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/ml concentration. Specific membrane-cytoplasmic immunopositivity of FGF R1 prot](http://images.novusbio.com/fullsize/FGFR1-Antibody-M17D10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80733-img0005.jpg "Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/ml concentration. Specific membrane-cytoplasmic immunopositivity of FGF R1 prot")
![Flow Cytometry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/FGFR1-Antibody-M17D10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80733-img0006.jpg "Flow Cytometry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - FGFR1 (M17D10) antibody was tested in NTERA2 cells at 1:100 with DyLight 488 (Green) using a 40X objective. Actin and nuclei were counterstained with Phalloidin 568 (Red) and DAPI (Blue), respectively. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/FGFR1-Antibody-M17D10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80733-img0002.jpg "Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - FGFR1 (M17D10) antibody was tested in NTERA2 cells at 1:100 with DyLight 488 (Green) using a 40X objective. Actin and nuclei were counterstained with Phalloidin 568 (Red) and DAPI (Blue), respectively. Image from the standard format of this antibody.")
![Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Analysis of FFPE tissue section of human normal skin using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. Cells of the epidermal layer showed a very strong membrane-cytoplasmic staining with relatively weak nuclear immunopositivity for for th](http://images.novusbio.com/fullsize/FGFR1-Antibody-M17D10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80733-img0004.jpg "Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Analysis of FFPE tissue section of human normal skin using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. Cells of the epidermal layer showed a very strong membrane-cytoplasmic staining with relatively weak nuclear immunopositivity for for th")
![Flow Cytometry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/mL) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/FGFR1-Antibody-M17D10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80733-img0007.jpg "Flow Cytometry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/mL) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown. Image from the standard format of this antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")