![Knockout Validated: FFAR4/GPR120 Antibody [NBP1-00858] - 3T3-L1 cells were exposed to DHA (100 i1/4M) for 2 d in the presence of the differentiation medium. The expression of GPR120, corin and ANP was measured in the GPR120 deficient adipocytes treated with DHA by Western blotting. Image collected and cropped by Citeab from the following publication (Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes. Int J Mol Sci (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FFAR4-GPR120-Antibody-Knockout-Validated-NBP1-00858-img0010.jpg "Knockout Validated: FFAR4/GPR120 Antibody [NBP1-00858] - 3T3-L1 cells were exposed to DHA (100 i1/4M) for 2 d in the presence of the differentiation medium. The expression of GPR120, corin and ANP was measured in the GPR120 deficient adipocytes treated with DHA by Western blotting. Image collected and cropped by Citeab from the following publication (Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes. Int J Mol Sci (2019) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, KD, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide made to an internal portion of the human GPR120 protein (between residues 200-300) [UniProt Q5NUL3] |
| Localization | FFAR4/GPR120 is a transmembrane protein with extracellular-helical-cytoplasmic domains |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FFAR4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Mouse | 04/02/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for FFAR4/GPR120 Antibody (NBP1-00858)Find related products by research area.
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![Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal epithelial cells showed an expected cytoplasmic staining (immunogen of NBP1-00858 corresponds to the cytoplasmic domain of GPR120 protein ), while some cells especially the goblet cells population depicted nuclear positivity also. The observed nuclear signal may be justified on the fact that - some GPCRs are known for nuclear translocation upon binding to extracellular or endogenous/non-secreted ligands, wherein they impact the trancriptional regulation via GPCR-heterotrimeric G-protein-effector complexes.](http://images.novusbio.com/fullsize/FFAR4-GPR120-Antibody-Immunohistochemistry-Paraffin-NBP1-00858-img0005.jpg "Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal epithelial cells showed an expected cytoplasmic staining (immunogen of NBP1-00858 corresponds to the cytoplasmic domain of GPR120 protein ), while some cells especially the goblet cells population depicted nuclear positivity also. The observed nuclear signal may be justified on the fact that - some GPCRs are known for nuclear translocation upon binding to extracellular or endogenous/non-secreted ligands, wherein they impact the trancriptional regulation via GPCR-heterotrimeric G-protein-effector complexes.")
![Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - Analysis of GPR120 (V259) antibody in extracts from LOVO cells.](http://images.novusbio.com/fullsize/FFAR4-GPR120-Antibody-Western-Blot-NBP1-00858-img0007.jpg "Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - Analysis of GPR120 (V259) antibody in extracts from LOVO cells.")
![Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 expression in mouse spleen cells (1), bone marrow (2), bone marrow derived DC (3) and macrophages (4). This image was submitted through a verified customer review.](http://images.novusbio.com/fullsize/FFAR4-GPR120-Antibody-Western-Blot-NBP1-00858-img0008.jpg "Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 expression in mouse spleen cells (1), bone marrow (2), bone marrow derived DC (3) and macrophages (4). This image was submitted through a verified customer review.")
![Immunocytochemistry/Immunofluorescence: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 antibody was tested at 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/FFAR4-GPR120-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00858-img0006.jpg "Immunocytochemistry/Immunofluorescence: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 antibody was tested at 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.")
![Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal epithelial cells showed an expected cytoplasmic staining (immunogen of NBP1-00858 corresponds to the cytoplasmic domain of GPR120 protein), while some cells especially the goblet cells population depicted nuclear positivity also. The observed nuclear signal may be justified on the fact that - some GPCRs are known for nuclear translocation upon binding to extracellular or endogenous/non-secreted ligands, wherein they impact the trancriptional regulation via GPCR-heterotrimeric G-protein-effector complexes.](http://images.novusbio.com/fullsize/FFAR4-GPR120-Antibody-Immunohistochemistry-Paraffin-NBP1-00858-img0003.jpg "Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal epithelial cells showed an expected cytoplasmic staining (immunogen of NBP1-00858 corresponds to the cytoplasmic domain of GPR120 protein), while some cells especially the goblet cells population depicted nuclear positivity also. The observed nuclear signal may be justified on the fact that - some GPCRs are known for nuclear translocation upon binding to extracellular or endogenous/non-secreted ligands, wherein they impact the trancriptional regulation via GPCR-heterotrimeric G-protein-effector complexes.")
![Western Blot: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858] - The combination of RA & omega -3 PUFAs induces G alpha q-P38 activation through RAR alpha & GPR40(A): Cells were treated with RA(20μM) + EPA(80μM) with or without GPR120-knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (B): Cells were treated with RA(20μM) + EPA(80μM) with or without GPR40-knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (C): Cells were treated with RA(20μM) + EPA(80μM) with or without RAR alpha -knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (D): Cells were treated with RA(20μM) + EPA(80μM) with or without RAR beta -knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (E): Cells were treated with RA(20μM) + EPA(80μM) with or without RAR gamma -knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (F): MCF-7 cells were treated with RA(20μM) + EPA(80μM) & their extracts fractionated using an iodixanol density gradient, as described in the Materials & Methods section. Each fraction was subjected to SDS-PAGE & immunoblot analysis using antibodies against the indicated proteins. (G): MCF-7 cells were pretreated with the indicated concentrations of methyl-beta -cyclodextrin (M beta CD) for 1 h, followed by 15 min treatment with RA(20μM) + EPA(80μM). Cell lysates were prepared & subjected to SDS-PAGE & immunoblot analysis. (H): MCF-7 cells were pretreated with the indicated concentrations of methyl-beta -cyclodextrin (M beta CD) for 1 h followed by 24h treatment with RA(20μM) + EPA(80μM), & then subjected to cell counts. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22629), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-00858_rabbit-polyclonal-ffar4-gpr120-antibody-310202416175226.jpg "Western Blot: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858] - The combination of RA & omega -3 PUFAs induces G alpha q-P38 activation through RAR alpha & GPR40(A): Cells were treated with RA(20μM) + EPA(80μM) with or without GPR120-knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (B): Cells were treated with RA(20μM) + EPA(80μM) with or without GPR40-knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (C): Cells were treated with RA(20μM) + EPA(80μM) with or without RAR alpha -knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (D): Cells were treated with RA(20μM) + EPA(80μM) with or without RAR beta -knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (E): Cells were treated with RA(20μM) + EPA(80μM) with or without RAR gamma -knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (F): MCF-7 cells were treated with RA(20μM) + EPA(80μM) & their extracts fractionated using an iodixanol density gradient, as described in the Materials & Methods section. Each fraction was subjected to SDS-PAGE & immunoblot analysis using antibodies against the indicated proteins. (G): MCF-7 cells were pretreated with the indicated concentrations of methyl-beta -cyclodextrin (M beta CD) for 1 h, followed by 15 min treatment with RA(20μM) + EPA(80μM). Cell lysates were prepared & subjected to SDS-PAGE & immunoblot analysis. (H): MCF-7 cells were pretreated with the indicated concentrations of methyl-beta -cyclodextrin (M beta CD) for 1 h followed by 24h treatment with RA(20μM) + EPA(80μM), & then subjected to cell counts. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22629), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858] - The expression of corin & ANP was increased in DHA-induced adipocytes. 3T3-L1 cells were exposed to DHA (100 μM) for 2 d in the presence of the differentiation medium. (A–C) The expression of corin & ANP in DHA-induced adipocytes was analyzed by qRT-PCR & Western blotting. The basal delta-Ct levels for tested genes are presented as Supplementary Table S2. ** p < 0.01. (D,E) The expression of corin & ANP was measured in the GPR120 deficient adipocytes treated with DHA by qRT-PCR & Western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001. (F) 3T3-L1 cells were treated with 1 μM TUG-891, a potent GPR120 agonist for 24 h. The corin & ANP expression levels were analyzed by Western blotting. (G) The concentration of ANP was measured in the media derived from the DHA-induced adipocytes using ELISA. ** p < 0.01. The data are shown as the means ± standard deviations from three or more independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31817347), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-00858_rabbit-polyclonal-ffar4-gpr120-antibody-31020241616567.jpg "Western Blot: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858] - The expression of corin & ANP was increased in DHA-induced adipocytes. 3T3-L1 cells were exposed to DHA (100 μM) for 2 d in the presence of the differentiation medium. (A–C) The expression of corin & ANP in DHA-induced adipocytes was analyzed by qRT-PCR & Western blotting. The basal delta-Ct levels for tested genes are presented as Supplementary Table S2. ** p < 0.01. (D,E) The expression of corin & ANP was measured in the GPR120 deficient adipocytes treated with DHA by qRT-PCR & Western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001. (F) 3T3-L1 cells were treated with 1 μM TUG-891, a potent GPR120 agonist for 24 h. The corin & ANP expression levels were analyzed by Western blotting. (G) The concentration of ANP was measured in the media derived from the DHA-induced adipocytes using ELISA. ** p < 0.01. The data are shown as the means ± standard deviations from three or more independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31817347), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
