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Fc gamma RII/RIII (CD32/CD16) Antibody [Biotin]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF1460
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF1460
Carrier Free
AF1460
Carrier Free
AF1460-SP
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Fc gamma RII/RIII (CD32/CD16) Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
Thr40-Arg217
Accession # NP_034317
Specificity
Detects mouse Fc gamma RII/RIII (CD32/CD16) in ELISAs and Western blots. In sandwich immunoassays, less than 10% cross-reactivity with recombinant mouse CD16 is observed and less than 0.1% cross-reactivity with recombinant human CD32 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse Fc gamma RIIB/CD32b Antibody (Catalog # MAB14601)
ELISA Detection: Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody (Catalog # BAF1460)
Standard:Recombinant Mouse Fc gamma RIIB/CD32b (Catalog # 1460-CD)
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RII/RIII (CD32/CD16) Antibody [Biotin]

  • CD16;CD16A;CD16-II;FCG3;FCGR3;FCGR3A;FCGRIII;FcGRIIIA;FCR-10;FCRIII;FCRIIIA;IGFR3;IMD20;Low affinity immunoglobulin gamma Fc region receptor III-A
  • CD32/CD16
  • Fc gamma RII/RIII

Background

Fc gamma RII, also named CD32, is a low-affinity receptor for the Fc region of complexed IgG. Three genes for human Fc gamma RII (A, B and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins, have been identified. The extracellular domain of mouse Fc gamma RII shares approximately 95% amino acid sequence identity with that of mouse Fc gamma RIII/CD16.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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