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Human Fc gamma RII/CD32 ELISA Kit (Colorimetric)

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ELISA: Human Fc gamma RII/CD32 ELISA Kit (Colorimetric) [NBP3-00382] - Samples were spiked with high concentrations of Human Fc gamma RII/CD32 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Fc gamma RII/CD32 ELISA Kit (Colorimetric) [NBP3-00382] - Samples were spiked with high concentrations of Human Fc gamma RII/CD32 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human Fc gamma RII/CD32 ELISA Kit (Colorimetric) [NBP3-00382] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
46.88 - 3000 pg/mL
Sensitivity
28.13 pg/mL

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Human Fc gamma RII/CD32 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Fc gamma RII/CD32 in samples. No significant cross-reactivity or interference between Human Fc gamma RII/CD32 and analogues was observed.
Standard Curve Range
46.88 - 3000 pg/mL
Sensitivity
28.13 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.01%
Intra-Assay
5.13%
Spike Recovery
89 - 107%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
FCGR2B

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Fc gamma RII/CD32 ELISA Kit (Colorimetric)

  • CD32 antigen
  • CD32B
  • CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
  • CDw32
  • Fc fragment of IgG, low affinity IIb, receptor (CD32)
  • Fc fragment of IgG, low affinity IIb, receptor for (CD32)
  • Fc gamma RII
  • Fc gamma RIIb
  • FCG2
  • Fc-gamma RII-b
  • FcgammaRIIb
  • fc-gamma-RIIb
  • FCGR2
  • FcgRII
  • FcgRIIB
  • fcRII-b
  • FcRII-c
  • IGFR2
  • IgG Fc receptor II-b
  • low affinity immunoglobulin gamma Fc region receptor II-b

Background

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Human Fc gamma RII/CD32 ELISA Kit (Colorimetric) Manuals
  PDF Datasheet
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Bioinformatics

Gene Symbol FCGR2B