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Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free

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Western Blot: Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free [NBP2-71722] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Exosome component 7 (Right lane) cDNA for 48 ...read more
Immunocytochemistry/ Immunofluorescence: Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free [NBP2-71722] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Exosome component 7.
Immunohistochemistry: Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free [NBP2-71722] - Staining of paraffin-embedded Human prostate tissue using anti-Exosome component 7 mouse monoclonal antibody.
Western Blot: Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free [NBP2-71722] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Exosome component 7 monoclonal antibody (HepG2: human; HeLa: ...read more
Immunohistochemistry: Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free [NBP2-71722] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Exosome component 7 mouse monoclonal antibody.
Immunohistochemistry: Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free [NBP2-71722] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Exosome component 7 mouse monoclonal antibody.
Immunohistochemistry: Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free [NBP2-71722] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Exosome component 7 mouse monoclonal antibody.
Immunohistochemistry: Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free [NBP2-71722] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Exosome component 7 mouse monoclonal antibody.
Immunohistochemistry: Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free [NBP2-71722] - Staining of paraffin-embedded Human Kidney tissue using anti-Exosome component 7 mouse monoclonal antibody.
Immunohistochemistry: Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free [NBP2-71722] - Staining of paraffin-embedded Human pancreas tissue using anti-Exosome component 7 mouse monoclonal antibody.
Immunohistochemistry: Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free [NBP2-71722] - Staining of paraffin-embedded Human tonsil using anti-Exosome component 7 mouse monoclonal antibody.
CyTOF-ready: Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free [NBP2-71722] - Analysis of Jurkat cells, using anti-Exosome component 7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
CyTOF-ready: Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free [NBP2-71722] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human EXOSC7(NP_055819) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EXOSC7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
31.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Exosome component 7 Antibody (OTI1G8) - Azide and BSA Free

  • EAP1
  • exosome complex exonuclease RRP42
  • exosome component 7Rrp42p
  • KIAA0116hRrp42p
  • p8FLJ26543
  • RRP42Ribosomal RNA-processing protein 42

Background

Exosome component 7 is a component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3'-untranslated regions. Required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA. H

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EXOSC7