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ERK4/MAPK4 Antibody (OTI2D7) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, ICC/IF
Clone
OTI2D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71240AF350
Alexa Fluor 405
NBP2-71240AF405
Alexa Fluor 488
NBP2-71240AF488
Alexa Fluor 532
NBP2-71240AF532
Alexa Fluor 594
NBP2-71240AF594
Alexa Fluor 647
NBP2-71240AF647
Alexa Fluor 700
NBP2-71240AF700
Alexa Fluor 750
NBP2-71240AF750
Allophycocyanin
NBP2-71240APC
Biotin
NBP2-71240B
CoraFluor 1
NBP2-71240CL1
DyLight 350
NBP2-71240UV
DyLight 405
NBP2-71240V
DyLight 488
NBP2-71240G
DyLight 550
NBP2-71240R
DyLight 594
NBP2-71240DL594
DyLight 650
NBP2-71240C
DyLight 680
NBP2-71240FR
DyLight 755
NBP2-71240IR
FITC
NBP2-71240F
HRP
NBP2-71240H
Janelia Fluor 525
NBP2-71240JF525
Janelia Fluor 585
NBP2-71240JF585
Janelia Fluor 635
NBP2-71240JF635
Janelia Fluor 646
NBP2-71240JF646
Janelia Fluor 669
NBP2-71240JF669
PE
NBP2-71240PE
PerCP
NBP2-71240PCP
Unconjugated
NBP2-45743
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45743
Azide and BSA Free
NBP2-71240
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ERK4/MAPK4 Antibody (OTI2D7) [Janelia Fluor® 549] Summary

Immunogen
Full length recombinant protein of human ERK4/MAPK4 (NP_002738) produced in HEK293T cells.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MAPK4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for ERK4/MAPK4 Antibody (OTI2D7) [Janelia Fluor® 549]

  • EC 2.7.11
  • EC 2.7.11.24
  • ERK3
  • Erk3-related
  • ERK4
  • ERK-4
  • Extracellular signal-regulated kinase 4
  • MAP kinase 4
  • MAP kinase isoform p63
  • MAPK 4
  • MAPK4
  • mitogen-activated protein kinase 4
  • p63Mapk
  • PRKM4
  • PRKM4p63-MAPK

Background

MAPK4 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAPK4