Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (SPM255) - IHC-Prediluted

Murine EHS laminin preparation

Basement membrane

This monoclonal antibody specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecans strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

IgG2a Kappa

Monoclonal

Rat

HSPG2

Protein A or G purified

Test in a species/application not listed above to receive a full credit towards a future purchase.

Hu-chromosome location: 1p36.1-p34 Molecular weight of antigen: >400kDa Immunohistochemistry-Paraffin 1 - 2 ug/ml for 30 minutes at RT; Staining of formalin/paraffin tissues Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

400 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.