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EHHADH Antibody (OTI2C4) - Azide and BSA Free

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Western Blot: EHHADH Antibody (OTI2C4) - Azide and BSA Free [NBP2-70625] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EHHADH.
Western Blot: EHHADH Antibody (OTI2C4) - Azide and BSA Free [NBP2-70625] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EHHADH.
Immunohistochemistry: EHHADH Antibody (OTI2C4) - Azide and BSA Free [NBP2-70625] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI2C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70625AF350
Alexa Fluor 405
NBP2-70625AF405
Alexa Fluor 488
NBP2-70625AF488
Alexa Fluor 532
NBP2-70625AF532
Alexa Fluor 594
NBP2-70625AF594
Alexa Fluor 647
NBP2-70625AF647
Alexa Fluor 700
NBP2-70625AF700
Alexa Fluor 750
NBP2-70625AF750
Allophycocyanin
NBP2-70625APC
Biotin
NBP2-70625B
CoraFluor 1
NBP2-70625CL1
DyLight 350
NBP2-70625UV
DyLight 405
NBP2-70625V
DyLight 488
NBP2-70625G
DyLight 550
NBP2-70625R
DyLight 594
NBP2-70625DL594
DyLight 650
NBP2-70625C
DyLight 680
NBP2-70625FR
DyLight 755
NBP2-70625IR
FITC
NBP2-70625F
HRP
NBP2-70625H
Janelia Fluor 525
NBP2-70625JF525
Janelia Fluor 549
NBP2-70625JF549
Janelia Fluor 585
NBP2-70625JF585
Janelia Fluor 635
NBP2-70625JF635
Janelia Fluor 646
NBP2-70625JF646
Janelia Fluor 669
NBP2-70625JF669
PE
NBP2-70625PE
PerCP
NBP2-70625PCP
Unconjugated
NBP2-46487
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46487
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EHHADH Antibody (OTI2C4) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 496-723 of human EHHADH (NP_001957) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EHHADH
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Theoretical MW
79.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for EHHADH Antibody (OTI2C4) - Azide and BSA Free

  • enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase
  • enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
  • LBFP
  • LBP
  • L-PBE
  • PBFEMGC120586
  • peroxisomal bifunctional enzyme3,2-trans-enoyl-CoA isomerase
  • peroxisomal enoyl-CoA hydratase
  • peroxisomal

Background

The protein encoded by the EHHADH gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomalbeta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while theC-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomaldisorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for thisgene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EHHADH