![Western Blot: EGR1 Antibody [NBP1-78775] - Whole cell lysate from mouse bone marrow dendritic (D; 50 and 15 mcg for WB; 1 mg for IP, 20% of IP loaded), NIH3T3 (M; 50 mcg), human erythroleukemia (E; 50 mcg), and Jurkat (J; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-EGR1 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EGR1 was also immunoprecipitated by rabbit anti-EGR1 antibody NBP1-78776 which recognizes a disparate epitope.](http://images.novusbio.com/fullsize/EGR1-Antibody-Western-Blot-NBP1-78775-img0002.jpg "Western Blot: EGR1 Antibody [NBP1-78775] - Whole cell lysate from mouse bone marrow dendritic (D; 50 and 15 mcg for WB; 1 mg for IP, 20% of IP loaded), NIH3T3 (M; 50 mcg), human erythroleukemia (E; 50 mcg), and Jurkat (J; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-EGR1 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EGR1 was also immunoprecipitated by rabbit anti-EGR1 antibody NBP1-78776 which recognizes a disparate epitope.")
| Reactivity | Hu, Mu, BvSpecies Glossary |
| Applications | WB, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen for this product maps to a region between residue 1 and 50 of mouse Early Growth Response Protein 1 using the numbering given in entry NP_031939.1 (GeneID 13653). |
| Predicted Species | Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EGR1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30846562). Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE. |
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| Theoretical MW | 56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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