Home » EBNA1BP2 » EBNA1BP2 Lysates » EBNA1BP2 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

EBNA1BP2 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: EBNA1BP2 Overexpression Lysate (Adult Normal) [NBL1-10093] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EBNA1BP2.
Western Blot: EBNA1BP2 Overexpression Lysate (Adult Normal) [NBL1-10093] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EBNA1BP2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

EBNA1BP2 Overexpression Lysate Summary

Description

EBNA1BP2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC200250

Accession#: NM_006824

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
EBNA1BP2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
34.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for EBNA1BP2 Overexpression Lysate

  • EBNA1 binding protein 2
  • EBNA1-binding protein 2nucleolar protein p40; homolog of yeast EBNA1-binding protein
  • EBP2nuclear FGF3 binding protein
  • NOBP
  • Nucleolar protein p40
  • P40
  • probable rRNA-processing protein EBP2

Background

EBNA1BP2 is required for the processing of the 27S pre-rRNA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

IL-23A/IL-23 P19
[Unconjugated]
1290-IL
IL-23A/IL-23 P19 [Unconjugated]
(115 Publications)
Species: Hu
Applications: BA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(452 Publications)
Species: Hu
Applications: BA
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(301 Publications)
Species: Mu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(811 Publications)
Species: Hu
Applications: BA
IL-12
[Unconjugated]
419-ML
SDS-PAGE IL-12 [Unconjugated]
Bioactivity IL-12 [Unconjugated]
(271 Publications)
Species: Mu
Applications: BA
CDK5 Activator 1
Antibody
NBP3-13279
Immunocytochemistry/ Immunofluorescence CDK5 Activator 1 Antibody
Immunohistochemistry-Paraffin CDK5 Activator 1 Antibody
Immunohistochemistry-Paraffin CDK5 Activator 1 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
CDCA5 Antibody
NBP1-89530
Western Blot CDCA5 Antibody
Immunohistochemistry-Paraffin CDCA5 Antibody
Immunocytochemistry/ Immunofluorescence CDCA5 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Annexin A1
Antibody [Unconjugate
AF3770
Western Blot Annexin A1 Antibody [Unconjugated]
Immunohistochemistry Annexin A1 Antibody [Unconjugated]
Simple Western Annexin A1 Antibody [Unconjugated]
(1 Review)
(6 Publications)
Species: Hu, Mu, Rt
Applications: IHC, Simple Western, WB
Ficolin-2
[Unconjugated]
2428-FC
Ficolin-2 [Unconjugated]
(2 Publications)
Species: Hu
Applications: Bind
Uroplakin IIIB
Antibody
NBP1-92564
Immunohistochemistry-Paraffin Uroplakin IIIB Antibody
Immunohistochemistry-Paraffin Uroplakin IIIB Antibody
Immunohistochemistry-Paraffin Uroplakin IIIB Antibody
(1 Review)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
GCHFR Antibody
(846011) [Unconjugate
MAB7918
Simple Western GCHFR Antibody (846011) [Unconjugated]
Western Blot GCHFR Antibody (846011) [Unconjugated]
Species: Hu
Applications: Simple Western, WB
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry p19ARF/CDKN2A Antibody - BSA Free
Western Blot p19ARF/CDKN2A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence p19ARF/CDKN2A Antibody - BSA Free
(1 Review)
(48 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(661 Publications)
Species: Mu
Applications: ELISA
IL-37/IL-1F7
[Biotin]
DY1975
N/A IL-37/IL-1F7 [Biotin]
(13 Publications)
Species: Hu
Applications: ELISA
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA
Annexin A6 Antibody
NBP1-90149
Western Blot Annexin A6 Antibody
Immunohistochemistry-Paraffin Annexin A6 Antibody
Immunohistochemistry-Paraffin Annexin A6 Antibody
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Factor IX Antibody
NBP1-33581
Immunohistochemistry-Paraffin Factor IX Antibody
Immunohistochemistry-Paraffin Factor IX Antibody
Immunocytochemistry/ Immunofluorescence Factor IX Antibody
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P
p19 INK4d Antibody
(DCS-100)
NBP3-11884
Immunocytochemistry/ Immunofluorescence p19 INK4d Antibody (DCS-100)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, RIA, WB
Reg1A Antibody
(431202) [Unconjugate
MAB4937
Intracellular Staining by Flow Cytometry Reg1A Antibody (431202) [Unconjugated]
(2 Publications)
Species: Hu
Applications: CyTOF-ready, ICFlow, WB

Publications for EBNA1BP2 Lysate (NBL1-10093) (0)

There are no publications for EBNA1BP2 Lysate (NBL1-10093).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EBNA1BP2 Lysate (NBL1-10093) (0)

There are no reviews for EBNA1BP2 Lysate (NBL1-10093). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EBNA1BP2 Lysate (NBL1-10093) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional EBNA1BP2 Products

Research Areas for EBNA1BP2 Lysate (NBL1-10093)

Find related products by research area.

Blogs on EBNA1BP2

There are no specific blogs for EBNA1BP2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EBNA1BP2 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol EBNA1BP2