Home » DYKDDDDK Epitope Tag » DYKDDDDK Epitope Tag Antibodies » DYKDDDDK Epitope Tag Antibody (1042E) [Alexa Fluor® 488]

DYKDDDDK Epitope Tag Antibody (1042E) [Alexa Fluor® 488]

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HEK293 cells transfected with DYKDDDDK-tagged proteins (filled histogram) vs Irrelevant HEK293 wild type cells (open histogram) were stained with Rabbit Anti-DYKDDDDK Epitope Tag Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8529G) at 0.001 ug/106 cells. To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.
HEK293 cells transfected with DYKDDDDK-tagged proteins (filled histogram) vs Irrelevant HEK293 wild type cells (open histogram) were stained with Rabbit Anti-DYKDDDDK Epitope Tag Alexa Fluor® 488‑conjugated ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications Flow
Clone
1042E
Clonality
Monoclonal
Host
Rabbit
Conjugate
Alexa Fluor 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC8529U-100UG
Alexa Fluor 405
IC8529V-100UG
Alexa Fluor 488
IC8529G-100
Alexa Fluor 594
IC8529T-100UG
Alexa Fluor 647
IC8529R-025
Alexa Fluor 647
IC8529R-100
Alexa Fluor 700
IC8529N-100UG
Alexa Fluor 750
IC8529S-100UG
Biotin
BAM8529
HRP
HAM85291
Unconjugated
MAB8529
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB8529
Carrier Free
IC8529R-025
Carrier Free
MAB8529
Carrier Free
HAM85291
Carrier Free
IC8529V-100UG
Carrier Free
IC8529T-100UG
Carrier Free
IC8529U-100UG
Carrier Free
IC8529S-100UG
Carrier Free
IC8529N-100UG
Carrier Free
IC8529R-100
Carrier Free
IC8529G-100
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DYKDDDDK Epitope Tag Antibody (1042E) [Alexa Fluor® 488] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
DYKDDDDK epitope tag
Specificity
Detects N-terminal and C-terminal DYKDDDDK (FLAG®) epitope tagged proteins in Western blot.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry
Publications
Read Publications using
IC8529G in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

FLAG® is a registered trademark of Sigma-Aldrich Co. LLC


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DYKDDDDK Epitope Tag Antibody (1042E) [Alexa Fluor® 488]

  • DDDDK epitope tag
  • DDK
  • DYKDDDDK Epitope Tag
  • DYKDDDDK tag
  • ECS epitope tag
  • ECS tag
  • Enterokinase Cleavage Site epitope tag
  • Enterokinase Cleavage Site tag
  • epitope tag flag
  • FLAG (FLAG is a trademark of Sigma Aldrich)
  • FLAG Tag
  • Ty 1 Tag
  • xxxDDDDK epitope tag

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DYKDDDDK Epitope Tag Antibody (IC8529G)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(4)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using IC8529G Applications Species
Wu, Z;Chen, G;Qiu, C;Yan, X;Xu, L;Jiang, S;Xu, J;Han, R;Shi, T;Liu, Y;Gao, W;Wang, Q;Li, J;Ye, F;Pan, X;Zhang, Z;Ning, P;Zhang, B;Chen, J;Du, Y; Structural basis for the ligand recognition and G protein subtype selectivity of kisspeptin receptor Science advances 2024-08-16 [PMID: 39151001] (Flow Cytometry, Human) Flow Cytometry Human
Kim, MS;Bhargava, HK;Shavey, GE;Lim, WA;El-Samad, H;Ng, AH; Degron-based bioPROTACs for controlling signaling in CAR T cells bioRxiv : the preprint server for biology 2024-02-17 [PMID: 38405763] (Flow Cytometry, Human, Mouse) Flow Cytometry Human, Mouse
Mitrut, RE;Stranford, DM;Chan, JM;Bailey, MD;Luo, M;Meade, TJ;Wang, M;Leonard, JN; HaloTag display enables quantitative single-particle characterization and functionalization of engineered extracellular vesicles bioRxiv : the preprint server for biology 2023-09-27 [PMID: 37808729] (Flow Cytometry, Human) Flow Cytometry Human
H Qiu, C Hosking, E Rothzerg, A Samantha, K Chen, V Kuek, H Jin, S Zhu, A Vrielink, K Lim, M Foley, J Xu ADR3, a next generation i-body to human RANKL, inhibits osteoclast formation and bone resorption The Journal of Biological Chemistry, 2023-01-09;0(0):102889. 2023-01-09 [PMID: 36634847] (Human) Human
AJ Stevens, AR Harris, J Gerdts, KH Kim, C Trentesaux, JT Ramirez, WL McKeithan, F Fattahi, OD Klein, DA Fletcher, WA Lim Programming Multicellular Assembly with Synthetic Cell Adhesion Molecules Nature, 2022-12-12;0(0):. 2022-12-12 [PMID: 36509107] (Flow Cytometry, Human) Flow Cytometry Human
Geoff P. O’Donoghue, Lukasz J. Bugaj, Warren Anderson, Kyle G. Daniels, David J. Rawlings, Wendell A. Lim T cells selectively filter oscillatory signals on the minutes timescale Proceedings of the National Academy of Sciences 3/2/2021 [PMID: 33627405]

Reviews for DYKDDDDK Epitope Tag Antibody (IC8529G) (0)

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FAQs for DYKDDDDK Epitope Tag Antibody (IC8529G). (Showing 1 - 5 of 5 FAQs).

  1. Are the FLAG Tag antibodies validated in Simple Western?
    • Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.
  2. What the theoretical molecular weight for FLAG Tag antibodies?
    • The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.
  3. Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.
    • If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.
  4. Will FLAG Tag antibodies recognize FLAG at the C-terminal end?
    • Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.
  5. Do FLAG Tag antibodies comes in lyophilized form?
    • The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

Secondary Antibodies

 

Isotype Controls

Additional DYKDDDDK Epitope Tag Products

Blogs on DYKDDDDK Epitope Tag.

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By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto...  Read full blog post.
Hoist the DYKDDDDK Epitope Tag
The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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