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Human Dopamine beta-Hydroxylase ELISA Kit (Colorimetric)

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ELISA: Human Dopamine beta-Hydroxylase ELISA Kit (Colorimetric) [NBP3-18755] - Samples were spiked with high concentrations of Human Dopamine beta-Hydroxylase and diluted with Reference Standard & Sample Diluent to ...read more
ELISA: Human Dopamine beta-Hydroxylase ELISA Kit (Colorimetric) [NBP3-18755] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
1.56-100 mIU/mL
Sensitivity
0.94 mIU/mL

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Human Dopamine beta-Hydroxylase ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Dopamine beta-Hydroxylase in samples. No significant cross-reactivity or interference between Human Dopamine beta-Hydroxylase and analogues was observed.
Standard Curve Range
1.56-100 mIU/mL
Sensitivity
0.94 mIU/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.80
Intra-Assay
%CV < 5.35
Spike Recovery
95-110%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
DBH

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard & Sample Diluent
  10. Reference Standard
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Dopamine beta-Hydroxylase ELISA Kit (Colorimetric)

  • DBH
  • DBM
  • dopamine beta-hydroxylase (dopamine beta-monooxygenase)
  • Dopamine betaHydroxylase
  • Dopamine beta-Hydroxylase
  • Dopamine beta-monooxygenase
  • DOPBHY
  • EC 1.14.17.1

Background

Dopamine beta-hydroxylase belongs to the copper type II, ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of dopamine to noradrenaline. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons being expressed in noradrenergic and adrenergic neurons. DBH serves as a marker of noradrenergic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol DBH