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DNAJA2 Antibody (OTI3A10) - Azide and BSA Free

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Western Blot: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJA2.
Western Blot: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJA2 (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJA2.
Immunohistochemistry: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - Staining of paraffin-embedded Human lymphoma tissue using anti-DNAJA2 mouse monoclonal antibody.
Flow Cytometry: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-DNAJA2 ...read more
Western Blot: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - Analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJA2 monoclonal antibody.
Immunohistochemistry: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DNAJA2 mouse monoclonal antibody.
Immunohistochemistry: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DNAJA2 mouse monoclonal antibody.
Immunohistochemistry: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DNAJA2 mouse monoclonal antibody.
Immunohistochemistry: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DNAJA2 mouse monoclonal antibody.
Immunohistochemistry: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - Staining of paraffin-embedded Human breast tissue using anti-DNAJA2 mouse monoclonal antibody.
Immunohistochemistry: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - Staining of paraffin-embedded Human Kidney tissue using anti-DNAJA2 mouse monoclonal antibody.
Immunohistochemistry: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - Staining of paraffin-embedded Human lymph node tissue using anti-DNAJA2 mouse monoclonal antibody.
Immunohistochemistry: DNAJA2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71565] - Staining of paraffin-embedded Human thyroid tissue using anti-DNAJA2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI3A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71565AF350
Alexa Fluor 405
NBP2-71565AF405
Alexa Fluor 488
NBP2-71565AF488
Alexa Fluor 532
NBP2-71565AF532
Alexa Fluor 594
NBP2-71565AF594
Alexa Fluor 647
NBP2-71565AF647
Alexa Fluor 700
NBP2-71565AF700
Alexa Fluor 750
NBP2-71565AF750
Allophycocyanin
NBP2-71565APC
Biotin
NBP2-71565B
CoraFluor 1
NBP2-71565CL1
DyLight 350
NBP2-71565UV
DyLight 405
NBP2-71565V
DyLight 488
NBP2-71565G
DyLight 550
NBP2-71565R
DyLight 594
NBP2-71565DL594
DyLight 650
NBP2-71565C
DyLight 680
NBP2-71565FR
DyLight 755
NBP2-71565IR
FITC
NBP2-71565F
HRP
NBP2-71565H
Janelia Fluor 525
NBP2-71565JF525
Janelia Fluor 549
NBP2-71565JF549
Janelia Fluor 646
NBP2-71565JF646
Janelia Fluor 669
NBP2-71565JF669
PE
NBP2-71565PE
PerCP
NBP2-71565PCP
Unconjugated
NBP2-02996
mFluor Violet 450 SE
NBP2-71565MFV450
mFluor Violet 500 SE
NBP2-71565MFV500
mFluor Violet 610 SE
NBP2-71565MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-02996
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DNAJA2 Antibody (OTI3A10) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human DNAJA2 (NP_005871) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DNAJA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50-150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Theoretical MW
45.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for DNAJA2 Antibody (OTI3A10) - Azide and BSA Free

  • cell cycle progression 3 protein
  • Cell cycle progression restoration gene 3 protein
  • CPR3PRO3015
  • Dj3
  • DJA2
  • DnaJ (Hsp40) homolog, subfamily A, member 2
  • dnaJ homolog subfamily A member 2
  • DNAJ
  • DNJ3
  • HIRA interacting protein 4
  • HIRA-interacting protein 4
  • HIRIP4RDJ2
  • Renal carcinoma antigen NY-REN-14

Background

DNAJA2 is encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DNAJA2 Antibody
(OTI3A10) - Azide and ...
NBP2-71565
DNAJA2 Antibody (OTI3A10) - Azide and BSA Free
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DNAJA2