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DNA Ligase I Antibody (1A9) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, IP
Clone
1A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08267AF350
Alexa Fluor 405
NBP3-08267AF405
Alexa Fluor 488
NBP3-08267AF488
Alexa Fluor 532
NBP3-08267AF532
Alexa Fluor 594
NBP3-08267AF594
Alexa Fluor 647
NBP3-08267AF647
Alexa Fluor 700
NBP3-08267AF700
Alexa Fluor 750
NBP3-08267AF750
Allophycocyanin
NBP3-08267APC
Biotin
NBP3-08267B
CoraFluor 1
NBP3-08267CL1
DyLight 350
NBP3-08267UV
DyLight 405
NBP3-08267V
DyLight 488
NBP3-08267G
DyLight 550
NBP3-08267R
DyLight 594
NBP3-08267DL594
DyLight 650
NBP3-08267C
DyLight 680
NBP3-08267FR
DyLight 755
NBP3-08267IR
FITC
NBP3-08267F
HRP
NBP3-08267H
Janelia Fluor 525
NBP3-08267JF525
Janelia Fluor 585
NBP3-08267JF585
Janelia Fluor 635
NBP3-08267JF635
Janelia Fluor 646
NBP3-08267JF646
Janelia Fluor 669
NBP3-08267JF669
PE
NBP3-08267PE
PerCP
NBP3-08267PCP
Unconjugated
NBP3-07248-100ug
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-07248-100ug
Azide and BSA Free
NBP3-08267
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DNA Ligase I Antibody (1A9) [Janelia Fluor® 549] Summary

Immunogen
Full-length native bovine DNA Ligase I protein (Uniprot: P18858)
Localization
Nucleus.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LIG1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for DNA Ligase I Antibody (1A9) [Janelia Fluor® 549]

  • DNA ligase 1
  • DNA Ligase I
  • EC 6.5.1.1
  • ligase I, DNA, ATP-dependent
  • MGC117397
  • MGC130025
  • Polydeoxyribonucleotide synthase [ATP] 1
  • polydeoxyribonucleotide synthase ATP 1

Background

DNA ligase 1 catalyzes the joining of double-stranded DNA ends during replication, recombination, and repair. Structurally, DNA ligase 1 is toroidal and encircles nicked DNA substrates. The 9-1-1 complex, a DNA damage checkpoint complex, has been reported to associate with DNA ligase 1 and stimulate ligase activity. Mutations in DNA ligase 1 result in immunodeficiency and increased sensitivity to DNA-damaging agents.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DNA Ligase I
Antibody (1A9) [Janel ...
NBP3-08267JF549
DNA Ligase I Antibody (1A9) [Janelia Fluor® 549]
Species: Hu, Mu, Rt, Bv
Applications: WB, ELISA, IP

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Secondary Antibodies

 

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Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and ...  Read full blog post.
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Bioinformatics

Gene Symbol LIG1