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DMC1 Antibody (2H12/4) - Azide and BSA Free

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Immunohistochemistry-Paraffin: DMC1 Antibody (2H12/4) - Azide and BSA Free [NBP3-08832] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with DMC1 Mouse Monoclonal Antibody (2H12/4).
Immunohistochemistry-Paraffin: DMC1 Antibody (2H12/4) - Azide and BSA Free [NBP3-08832] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with DMC1 Mouse Monoclonal Antibody (2H12/4).
Immunohistochemistry-Paraffin: DMC1 Antibody (2H12/4) - Azide and BSA Free [NBP3-08832] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with DMC1 Mouse Monoclonal Antibody (2H12/4).

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
2H12/4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

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DMC1 Antibody (2H12/4) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07507).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant full-length human DMC1 protein. (Uniprot: Q14565)
Localization
Nucleus. Chromosome.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DMC1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for DMC1 Antibody (2H12/4) - Azide and BSA Free

  • disrupted meiotic cDNA1, yeast, homolog of
  • DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologousrecombination (yeast)
  • DMC1 homologue
  • HsLim15
  • LIM15yeast homolog) meiosis-specific homologousrecombination
  • meiotic recombination protein DMC1/LIM15 homolog
  • MGC150472
  • MGC150473

Background

DMC-1 is a meiosis-specific homologue of RecA/Rad51 and is an essential component of the meiotic recombination machinery in yeast and higher eukaryotes. DMC-1 is the human equivalent of e. coli recA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DMC1 Antibody (NBP3-08832) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DMC1 Antibody (NBP3-08832) (0)

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Secondary Antibodies

 

Isotype Controls

Additional DMC1 Products

Research Areas for DMC1 Antibody (NBP3-08832)

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Blogs on DMC1.

Determining DMC1's role in Homologus Recombination
The DMC1 gene encodes a 36.7 kDa nuclear protein involved in meiotic homologous recombination. This recombinase is functionally related to the yeast RAD51 and E. coli RecA genes. In contrast to RAD51, which functions in both mitotic and meiotic recomb...  Read full blog post.

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Bioinformatics

Gene Symbol DMC1