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DGK-epsilon Antibody (7E1)

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Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03] - Analysis of DGKE expression in Raw 264.7 (Cat # L024V1).
Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03] - Analysis of DGKE expression in HeLa (Cat # L013V1).
Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03] - Analysis of DGKE expression in PC-12 (Cat # L012V1).
Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03] - Analysis of DGKE expression in NIH/3T3 (Cat # L018V1).
Sandwich ELISA: DGK-epsilon Antibody (7E1) [H00008526-M03] - Detection limit for recombinant GST tagged DGKE is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
7E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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DGK-epsilon Antibody (7E1) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
DGKE (NP_003638, 141 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CKQQCGCQPKLCDYRCIWCQKTVHDECMKNSLKNEKCDFGEFKNLIIPPSYLTSINQMRKDKKTDYEVLASKLGKQWTPLIILANSRSGTNMGEGLLGEF
Specificity
DGKE - diacylglycerol kinase, epsilon 64kDa
Isotype
IgG3 Lambda
Clonality
Monoclonal
Host
Mouse
Gene
DGKE
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 23542698).
Publications
Read Publication using
H00008526-M03 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DGK-epsilon Antibody (7E1)

  • DAG kinase epsilon
  • DAGK5
  • DAGK6
  • DGK
  • DGKE
  • DGKepsilon
  • DGK-epsilon
  • diacylglycerol kinase epsilon
  • diacylglycerol kinase, epsilon (64kD)
  • diacylglycerol kinase, epsilon 64kDa
  • Diglyceride kinase epsilon
  • EC 2.7.1.107

Background

Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DGK-epsilon Antibody (H00008526-M03)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Human
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Bioinformatics

Gene Symbol DGKE
Entrez
OMIM
Uniprot