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Decorin Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Decorin Antibody [NBP1-84970] - Staining in human ovary and cerebral cortex tissues using anti-DCN antibody. Corresponding DCN RNA-seq data are presented for ...read more
Western Blot: Decorin Antibody [NBP1-84970] - Analysis in control (vector only transfected HEK293T lysate) and DCN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Western Blot: Decorin Antibody [NBP1-84970] - Analysis in human cell line U-2197.
Immunohistochemistry-Paraffin: Decorin Antibody [NBP1-84970] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: Decorin Antibody [NBP1-84970] - Staining of human ovary shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Decorin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DCN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 25979340). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Decorin Protein (NBP1-84970PEP)
Publications
Read Publications using
NBP1-84970 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24634138).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Decorin Antibody

  • Bone proteoglycan II
  • CSCD
  • DCN
  • decorin proteoglycan
  • Decorin
  • dermatan sulphate proteoglycans II
  • DSPG2
  • PG40
  • PGII
  • PG-II
  • PGS2
  • PG-S2
  • proteoglycan core protein
  • SLRR1B
  • SLRR1BPGS2
  • small leucine-rich protein 1B

Background

Decorin is encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Decorin Antibody (NBP1-84970)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC-P, Immunohistochemistry, WB.


Filter By Application
IHC-P
(1)
Immunohistochemistry
(1)
WB
(1)
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Human
(3)
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Showing Publications 1 - 7 of 7.
Publications using NBP1-84970 Applications Species
Voutilainen SH, Kosola SK, Lohi J et al. Expression of 6 Biomarkers in Liver Grafts After Pediatric Liver Transplantation: Correlations with Histology, Biochemistry, and Outcome Annals of Transplantation 2020-10-16 [PMID: 33060556] (Immunohistochemistry) Immunohistochemistry
Kerola A, Lohi J, Heikkila P et al. Divergent expression of liver transforming growth factor superfamily cytokines after successful portoenterostomy in biliary atresia. Surgery 2019-01-24 [PMID: 30686515] (IHC-P, Human) IHC-P Human
Herchenhan A, Uhlenbrock F, Eliasson P et al. Lysyl Oxidase activity is required for ordered collagen fibrillogenesis by tendon cells. J. Biol. Chem. 2015-05-15 [PMID: 25979340] (WB, Human)

Details:
Tenascin X antibody was used at 1:500 dilution with ON 4C incubation for WB detection of established collagen fibrillogenesis regulator Tenascin X protein on samples from the control or beta-aminoproprionitrile /BAPN treated tendon constructs which were prepared using tendon fibroblasts isolated from human semitendinosus and gracilis tendon of human anterior cruciate ligament/ACL rupture patients undergoing reconstructive surgery. See the full text for protocol related details and the data is shown in FIGURE 7A.
WB Human
Bozoky B, Savchenko A, Guven H et al. Decreased decorin expression in the tumor microenvironment. Cancer Med 2014-06-01 [PMID: 24634138] (Human) Human
Henke A, Grace OC, Ashley GR et al. Stromal expression of decorin, Semaphorin6D, SPARC, Sprouty1 and Tsukushi in developing prostate and decreased levels of decorin in prostate cancer. PLoS One 2012-01-01 [PMID: 22880013]
Edlund K, Lindskog C, Saito A et al. CD99 is a novel prognostic stromal marker in non-small cell lung cancer. Int J Cancer 2012-11-15 [PMID: 22392539]
Cawthorn TR, Moreno JC, Dharsee M et al. Proteomic Analyses Reveal High Expression of Decorin and Endoplasmin (HSP90B1) Are Associated with Breast Cancer Metastasis and Decreased Survival. PLoS One 2012-01-01 [PMID: 22363530]

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol DCN