Recombinant Human DCI His Protein

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SDS-Page: DCI Protein [NBP2-23023]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

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Recombinant Human DCI His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 42-302 of Human DCI

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSFGSQRVL VEPDAGAGVA VMKFKNPPVN SLSLEFLTEL VISLEKLEND KSFRGVILTS DRPGVFSAGL DLTEMCGRSP AHYAGYWKAV QELWLRLYQS NLVLVSAING ACPAGGCLVA LTCDYRILAD NPRYCIGLNE TQLGIIAPFW LKDTLENTIG HRAAERALQL GLLFPPAEAL QVGIVDQVVP EEQVQSTALS AIAQWMAIPD HARQLTKAMM RKATASRLVT QRDADVQNFV SFISKDSIQK SLQMYLERLK EEKG

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ECI1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
31.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human DCI His Protein

  • acetylene-allene isomerase
  • D2-enoyl-CoA isomerase
  • D3
  • DCI3,2-trans-enoyl-CoA isomerase3,2 trans-enoyl-CoA isomerase
  • Delta(2)-enoyl-CoA isomerase
  • Delta(3)
  • delta3, delta2-enoyl-CoA isomerase
  • dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase)
  • dodecenoyl-CoA isomerase3,2-trans-enoyl-CoA isomerase, mitochondrial
  • dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
  • EC 5.3.3.8
  • enoyl-CoA delta isomerase 13,2 trans-enoyl-Coenzyme A isomerase

Background

ECI1 is a member of the hydratase/isomerase superfamily. The protein is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. Recombinant human ECI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol ECI1