![Western Blot: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Human HeLa lysate probed with Cytochrome C antibody at 0.1 ug/ml. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Western-Blot-NBP2-80686-img0002.jpg "Western Blot: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Human HeLa lysate probed with Cytochrome C antibody at 0.1 ug/ml. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, Dr, RbSpecies Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC, WB, CyTOF-ready |
| Clone | 7H8.2C12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991). |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CYCS |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytochrome c Antibody (NBP2-80686)Find related products by research area.
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Pathway Highlight: Three key factors that contribute to cellular heterogeneity in apoptosis Have you ever wondered why cells in the same population respond differently to an apoptotic stimulus? Apoptosis, a form of programmed cell death, is vital for the removal of unwanted or damaged cells. As with most cellular processes, too much or to... Read full blog post. |
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The use of apoptosis antibodies and controls in cell death research Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance. Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha... Read full blog post. |
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Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co... Read full blog post. |
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Cytochrome C - a mediator of apoptosis Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain. Additionally, cytochrome c has also been identified as a player in programmed cell death (apop... Read full blog post. |
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Cytochrome C in Apoptosis, Immune Response and Cancer Cytochrome C is an electron carrier protein that localizes in mitochondrion intermembrane space and has been identified as one of the key signaling molecules of apoptosis or programmed cell death. Suppression of the anti-apoptotic members or activatio... Read full blog post. |
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Mitofilin and the Mitochondrial Inner Membrane Organizing System (MINOS) Mitofilin was originally described as a heart muscle protein due to its high expression in the heart. Recently, analysis of the human heart mitochondrial proteome demonstrated that Mitofilin is one of the most abundant mitochondrial proteins (1). Rese... Read full blog post. |
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Bax Research Gives New Insight into Oxidative Apoptosis Bax is a member of the Bcl-2 family; an extensive range of proteins which play key roles in apoptosis, or programmed cell death, by regulating outer mitochondrial membrane permeability. We at Novus Biologicals are one of the leading antibody suppliers... Read full blog post. |
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The Role of the Caspase 3 Antibody in Apoptosis Research The caspases are a group of cysteine protease enzymes essential to apoptosis, inflammation and necrosis. Caspase 3 has been identified as one of the key mediators of apoptosis.Human caspases form three distinct groups: cytokine activators, which are... Read full blog post. |
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| Gene Symbol | CYCS |
![Immunocytochemistry/Immunofluorescence: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - p73 was detected in immersion fixed Hela human cell line using NB100-56503 at 25 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and mitochondria. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80686-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - p73 was detected in immersion fixed Hela human cell line using NB100-56503 at 25 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and mitochondria. Image from the standard format of this antibody.")
![Immunohistochemistry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Cytochrome C was detected in immersion fixed paraffin-embedded sections of human heart using anti-human mouse monoclonal antibody (Catalog # NB100-56503) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detec](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80686-img0004.jpg "Immunohistochemistry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Cytochrome C was detected in immersion fixed paraffin-embedded sections of human heart using anti-human mouse monoclonal antibody (Catalog # NB100-56503) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detec")
![Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Using the Alexa Fluor 488 direct conjugate, an intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80686-img0006.jpg "Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Using the Alexa Fluor 488 direct conjugate, an intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.")
![Western Blot: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Analysis using the biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 mg of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is detected.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Western-Blot-NBP2-80686-img0001.jpg "Western Blot: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Analysis using the biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 mg of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is detected.")
![Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Detection of Cytochrome C in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Cytochrome C Monoclonal Antibody (Catalog # NB100-56503, filled histogram), or Mouse IgG2B isotype control (Catalog # MAB0041, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a Bio-Techne brand. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80686-img0005.jpg "Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Detection of Cytochrome C in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Cytochrome C Monoclonal Antibody (Catalog # NB100-56503, filled histogram), or Mouse IgG2B isotype control (Catalog # MAB0041, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a Bio-Techne brand. Image from the standard format of this antibody.")
![Simple Western: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytochrome c at approximately 23 kDa (as indicated) using 2.5 ug/mL of Mouse Anti-Cytochrome c Monoclonal Antibody (Catalog # NB100-56503). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Azide-and-BSA-Free-Simple-Western-NBP2-80686-img0007.jpg "Simple Western: Cytochrome c Antibody (7H8.2C12) - Azide and BSA Free [NBP2-80686] - Lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytochrome c at approximately 23 kDa (as indicated) using 2.5 ug/mL of Mouse Anti-Cytochrome c Monoclonal Antibody (Catalog # NB100-56503). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Image from the standard format of this antibody.")
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