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CXCL8/IL-8 Antibody (105105) [Unconjugated]

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Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant PorcineIL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351). The ND50 is typically 1-6 µg/mL.
Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
105105
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5351-SP
Carrier Free
MAB5351-500
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CXCL8/IL-8 Antibody (105105) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant porcine IL-8/CXCL8
Ala26-Gln104
Accession # CAA43461
Specificity
Detects porcine IL-8/CXCL8 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha , 12/SDF-1 beta , rhCXCL13, recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha , rmCXCL13, recombinant rat (rr) CXCL1, rrCXCL3/CINC2 alpha , or rr3/CINC-2 beta is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL8
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 1-6 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Porcine CXCL8/IL-8 Biotinylated Antibody (Catalog number BAF535)
Standard: Recombinant Porcine CXCL8/IL-8 (Catalog number 535-IN)
Publications
Read Publications using
MAB5351 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL8/IL-8 Antibody (105105) [Unconjugated]

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAP
  • MONAPGCP1
  • NAF
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

Interleukin 8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro‑inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.

IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro‑inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. Recently, IL-8 was reported to be angiogenic both in vivo and in vitro.

  1. Van Damme, J. et al. (1998) in The Cytokine Handbook, A.W. Thomson ed., Academic Press, New York. p. 271.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL8/IL-8 Antibody (MAB5351)(9)

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 5 applications: ELISA Capture, ELISA Development, ELISA Development (Capture), Luminex Development, Western Blot.


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ELISA Capture
(2)
ELISA Development
(2)
ELISA Development (Capture)
(1)
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(1)
Western Blot
(1)
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Human
(1)
Porcine
(6)
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Showing Publications 1 - 9 of 9.
Publications using MAB5351 Applications Species
DE Marin, CV Bulgaru, CA Anghel, GC Pistol, MI Dore, ML Palade, I Taranu Grape Seed Waste Counteracts Aflatoxin B1 Toxicity in Piglet Mesenteric Lymph Nodes Toxins, 2020-12-15;12(12):. 2020-12-15 [PMID: 33333857] (ELISA Capture, Porcine) ELISA Capture Porcine
SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines MethodsX, 2019-05-17;6(0):1218-1227. 2019-05-17 [PMID: 31193967] (ELISA Capture, Porcine) ELISA Capture Porcine
Z Kunicka, A Kurzy?ska, A Szyd?owska, B Kaczy?ska, I Bogacka PPAR?/? ligands regulate the expression of immune response mediators in the porcine endometrium - An in�vitro study Theriogenology, 2019-05-28;134(0):112-120. 2019-05-28 [PMID: 31158734] (Western Blot, Porcine) Western Blot Porcine
S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets Infect. Immun., 2017-03-23;85(4):. 2017-03-23 [PMID: 28167667] (ELISA Development (Capture), Porcine) ELISA Development (Capture) Porcine
Daniela E. Marin, Monica Motiu, Ionelia Taranu Food Contaminant Zearalenone and Its Metabolites Affect Cytokine Synthesis and Intestinal Epithelial Integrity of Porcine Cells Toxins (Basel) 2015-05-29 [PMID: 26035492]
Ionelia Taranu, Mihail Gras, Gina Cecilia Pistol, Monica Motiu, Daniela E. Marin, Nicoleta Lefter et al. omega -3 PUFA Rich Camelina Oil By-Products Improve the Systemic Metabolism and Spleen Cell Functions in Fattening Pigs PLoS ONE 2014-10-10 [PMID: 25303320]
Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia. Intensive Care Med, 2007-04-25;33(6):1085-93. 2007-04-25 [PMID: 17457574] (ELISA Development, Human) ELISA Development Human
Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology. Cytometry A, 2006-05-01;69(5):391-5. 2006-05-01 [PMID: 16604540] (Luminex Development, Porcine) Luminex Development Porcine
Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Schmitz O, Orskov H, Tonnesen E Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model. Anesthesiology, 2004-04-01;100(4):861-70. 2004-04-01 [PMID: 15087621] (ELISA Development, Porcine) ELISA Development Porcine

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Bioinformatics

Gene Symbol IL8
Uniprot