![Western Blot: CT45A1 Antibody [NBP2-46702] - Analysis in human cell line U-2 OS and human cell line A-549.](http://images.novusbio.com/fullsize/CT45A1-Antibody-Western-Blot-NBP2-46702-img0012.jpg "Western Blot: CT45A1 Antibody [NBP2-46702] - Analysis in human cell line U-2 OS and human cell line A-549.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: TEKVAVDPETVFKRPRECDSPSYQKRQRMALLARKQGAGDSLIAGSAMSK |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CT45A1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Western Blot SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Western_Blot_5161.jpg)
![Chromatin Immunoprecipitation (ChIP) SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Chromatin_Immunoprecipitation_11224.jpg)
![Simple Western SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Simple_Western_16676.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: CT45A1 Antibody [NBP2-46702] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CT45A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-46702-img0011.jpg "Immunocytochemistry/Immunofluorescence: CT45A1 Antibody [NBP2-46702] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: CT45A1 Antibody [NBP2-46702] - Staining in human testis and liver tissues . Corresponding CT45A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CT45A1-Antibody-Immunohistochemistry-Paraffin-NBP2-46702-img0010.jpg "Immunohistochemistry-Paraffin: CT45A1 Antibody [NBP2-46702] - Staining in human testis and liver tissues . Corresponding CT45A1 RNA-seq data are presented for the same tissues.")
![Western Blot: CT45A1 Antibody [NBP2-46702] - Analysis NBP2-46702 (A) shows similar pattern to independent antibody NBP2-46729 (B).](http://images.novusbio.com/fullsize/CT45A1-Antibody-Western-Blot-NBP2-46702-img0013.jpg "Western Blot: CT45A1 Antibody [NBP2-46702] - Analysis NBP2-46702 (A) shows similar pattern to independent antibody NBP2-46729 (B).")
![Immunohistochemistry-Paraffin: CT45A1 Antibody [NBP2-46702] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CT45A1-Antibody-Immunohistochemistry-Paraffin-NBP2-46702-img0008.jpg "Immunohistochemistry-Paraffin: CT45A1 Antibody [NBP2-46702] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: CT45A1 Antibody [NBP2-46702] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CT45A1-Antibody-Immunohistochemistry-Paraffin-NBP2-46702-img0009.jpg "Immunohistochemistry-Paraffin: CT45A1 Antibody [NBP2-46702] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.")
![Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Chromatin_Immunoprecipitation_11218.jpg)
![Immunocytochemistry Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Immunocytochemistry_7093.jpg)
![Simple Western Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct3_4_AF1759_Simple_Western_20183.jpg)
![SDS-PAGE ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/ErbB2_1129-ER_70.jpg)
![Bioactivity ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/1129-er_recombinant-human-erbb2-her2-fc-chimera-protein-cf-bioactivity-7122020142841.jpg)
![Immunocytochemistry Progesterone R/NR3C3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Progesterone_R_AF5415_Immunocytochemistry__Immunofluorescence_20674.jpg)
![Immunohistochemistry Progesterone R/NR3C3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Progesterone_R_AF5415_Immunohistochemistry_20779.jpg)
![Western Blot Progesterone R/NR3C3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Progesterone_R_AF5415_Western_Blot_5715.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")