![Immunocytochemistry/Immunofluorescence: CRYBB2 Antibody [NBP2-13876] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CRYBB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13876-img0005.jpg "Immunocytochemistry/Immunofluorescence: CRYBB2 Antibody [NBP2-13876] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm. Antibody staining is shown in green.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: IIDDDVPSFHAHGYQEKVSSVWVRSG |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CRYBB2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP2-13876 | Applications | Species |
|---|---|---|
| Barrow MA, Martin ME, Coffey A et al. A functional role for the cancer disparity-linked genes, CRY beta B2 and CRY beta B2P1, in the promotion of breast cancer Breast Cancer Res. 2019-09-11 [PMID: 31511085] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CRYBB2 |
![Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/CRYBB2-Antibody-Immunohistochemistry-Paraffin-NBP2-13876-img0009.jpg "Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876] - Staining of human cerebral cortex shows no positivity in neurons as expected.")
![Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876] - Staining of human eye shows strong positivity in the lens.](http://images.novusbio.com/fullsize/CRYBB2-Antibody-Immunohistochemistry-Paraffin-NBP2-13876-img0006.jpg "Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876] - Staining of human eye shows strong positivity in the lens.")
![Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876] - Staining of human pancreas shows on positivity in exorcirne glanuldar cells as expected.](http://images.novusbio.com/fullsize/CRYBB2-Antibody-Immunohistochemistry-Paraffin-NBP2-13876-img0007.jpg "Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876] - Staining of human pancreas shows on positivity in exorcirne glanuldar cells as expected.")
![Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/CRYBB2-Antibody-Immunohistochemistry-Paraffin-NBP2-13876-img0008.jpg "Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")