Western Blot: Cre Antibody - Unpurified [NB100-56134] - Lane 1: wild-type mouse (negative control). Lanes 2 and 3: brain from 2 types of Cre transgenic mice. Lane 4: recombinant Cre (positive control). Full-length Cre ...read more
Immunohistochemistry: Cre Antibody - Unpurified [NB100-56134] - Cre Antibody [NB100-56134] - Analysis of Cre in a FFPE tissue section from the hippocampus of a Cre transgenic mouse at 1:2000. In this mouse line, Cre is ...read more
Western Blot: Cre Antibody [NB100-56134] - Generation, identification, & Rpe65 expression analysis of Rpe65CreERT2 mice.(A) KI strategy to introduce a P2A-CreERT2 coding sequence in-frame with the final coding exon ...read more
Immunohistochemistry Free-Floating reported in scientific literature (PMID 25429143)
Immunohistochemistry 1:10 - 1:5000
Immunohistochemistry-Frozen 1:10 - 1:5000
Immunohistochemistry-Paraffin 1:1000 - 1:5000
Immunoprecipitation 1:50 - 1:200
SDS-Page reported in scientific literature (PMID 27432829)
Western Blot 1:1000 - 1:2000. Use reported in scientific literature (PMID 33784255)
Application Notes
CRE is detected at approx. 36-38 kDa on western blots. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
38.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5 using NB100-56134 in the following applications:
Specific to Cre/lox system (Bacteriophage). Shown to detect Cre protein in Cre-transgenic mice. Use in Mouse reported in scientific literature (PMID:33784255)
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
50 uL neat whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified
Alternate Names for Cre Antibody
Cyclization recombinase
Background
The Cre (cyclization recombination) enzyme, a member of a large family of recombinases, recognizes loxP which is a sequence motif of 34 bp from the PI bateriophage. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule. The excised DNA segment is subsequently degraded. Cre and lox comprise the Cre/lox system which was first developed in the late 1980's to artificially manipulate gene expression (reviewed in Branda and Dymecki, 2004; Ghosh and Duyne, 2002; and Gilbertson, 2003). As an example, mice with the Cre protein expressing in a specific cell type are bred with mice that contain a target gene surrounded by loxP sites. When the mice are bred, the cells carrying Cre will cause those cells to lose the target gene. If the Cre gene is, for example, bound to a promoter that only allows Cre production in neuronal cells, the target gene will be deleted only in those cells. Since 1989 the Cre/lox system has been used extensively and there are numerous animal, plant and bacterial stocks that already contain the Cre gene driven by ubiquitous, tissue-specific or inducible promoters and provide a quick method for breeding experiments. The Cre/lox system has the advantage of working in almost any type of cell. This has led to its application in many experiments, for example in selectively labeling neuronal cells in the brain thereby differentiating them from other types of surrounding cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Cre Antibody (NB100-56134)(8)
We have publications tested in 2 confirmed species: Mouse, Cre/lox System.
We have publications tested in 7 applications: Block/Neutralize, IF/IHC, IHC-Fr, IHC-FrFl, PAGE, WB, Western Blot.
FAQs for Cre Antibody (NB100-56134). (Showing 1 - 2 of 2 FAQs).
A customer is looking for a Cre Antibody, raised in either rabbit or goat, suitable for IHC-F. Would you know of a suitable Cre antibody that fits the above criteria and also been published for IHC-Fr? If known, can you also provide details of the publication?
With regards to your customer's inquiry on our Cre antibodies, we do have a wide range of antibodies that would be suitable to use in IHC for frozen sections. NB100-56134 for instance has been tested and validated for IHC-fry and there is one cited PubMed publication on it as well.
Do you have a recommended antigen retrieval protocol for NB100-56134?
The recommended antigen retrieval for tissues fixed in formalin and paraffin-embedded can be found below (this step is after rehydration and before blocking and primary incubation): Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100 degrees C - 20 minutes.Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) - 1 minute @ RT.
Understanding DNA Recombination with Cre-Lox Cyclization recombination enzyme (Cre) is a member of the extensive family of recombinases and recognizes a 34 bp sequence motif from PI bacteriophage referred to as LoxP. The Cre enzyme works to cleanly excise an intervening DNA fragment that is flan... Read full blog post.
Cre/Lox: The Genomic Utility Knife Cre (Cyclization recombination enzyme) is a member of the large family of recombinases. Cre recognizes Lox site loxP, a 34 bp sequence motif from the PI bateriophage. If a DNA segment is flanked by two loxP sites in the same orientation, Cre neatly ex... Read full blog post.
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![Western Blot: Cre Antibody - Unpurified [NB100-56134] - Lane 1: wild-type mouse (negative control). Lanes 2 and 3: brain from 2 types of Cre transgenic mice. Lane 4: recombinant Cre (positive control). Full-length Cre is detected at ~36-38 kDa. The lower molecular weight bands in lane 4 are breakdown products from the recombinant Cre protein.](http://images.novusbio.com/fullsize/Cre-Antibody-Western-Blot-NB100-56134-img0005.jpg "Western Blot: Cre Antibody - Unpurified [NB100-56134] - Lane 1: wild-type mouse (negative control). Lanes 2 and 3: brain from 2 types of Cre transgenic mice. Lane 4: recombinant Cre (positive control). Full-length Cre is detected at ~36-38 kDa. The lower molecular weight bands in lane 4 are breakdown products from the recombinant Cre protein.")
![Immunohistochemistry: Cre Antibody - Unpurified [NB100-56134] - Cre Antibody [NB100-56134] - Analysis of Cre in a FFPE tissue section from the hippocampus of a Cre transgenic mouse at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. Hematoxylin-eosin counterstain. A1 is a higher magnification of A.](http://images.novusbio.com/fullsize/Cre-Antibody-Immunohistochemistry-NB100-56134-img0004.jpg "Immunohistochemistry: Cre Antibody - Unpurified [NB100-56134] - Cre Antibody [NB100-56134] - Analysis of Cre in a FFPE tissue section from the hippocampus of a Cre transgenic mouse at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. Hematoxylin-eosin counterstain. A1 is a higher magnification of A.")
![Western Blot: Cre Antibody [NB100-56134] - Generation, identification, & Rpe65 expression analysis of Rpe65CreERT2 mice.(A) KI strategy to introduce a P2A-CreERT2 coding sequence in-frame with the final coding exon (exon 14) of the Rpe65 gene. Primer-binding sites & expected PCR product sizes are shown below the wild-type & targeted alleles. The neomycin cassette in the targeting vector was removed during expansion of the embryonic stem cell clone. FLP-recombinase was subsequently bred out by crossing with C57BL/6 mice. The KI allele allows cotranslational expression of RPE65-P2A & CreERT2 as 2 separate polypeptides. The modified RPE65 protein contains an additional G534SGATNFSLLKQAGDVEENPG554 polypeptide sequence at its C-terminus, while CreERT2 contains an additional Pro residue at its N-terminus. (B) Genotyping results from wild-type, Cre+/–, & Cre+/+ animals. (C) Western blot analysis of RPE65 expression. After normalization to the alpha -tubulin loading controls, the RPE65 expression levels in Cre+/– & Cre+/+ mice were estimated to be 59.7% & 1.1% of that for wild-type mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33784255), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56134_rabbit-polyclonal-cre-antibody-imgenex-img-5721-31020241555211.jpg "Western Blot: Cre Antibody [NB100-56134] - Generation, identification, & Rpe65 expression analysis of Rpe65CreERT2 mice.(A) KI strategy to introduce a P2A-CreERT2 coding sequence in-frame with the final coding exon (exon 14) of the Rpe65 gene. Primer-binding sites & expected PCR product sizes are shown below the wild-type & targeted alleles. The neomycin cassette in the targeting vector was removed during expansion of the embryonic stem cell clone. FLP-recombinase was subsequently bred out by crossing with C57BL/6 mice. The KI allele allows cotranslational expression of RPE65-P2A & CreERT2 as 2 separate polypeptides. The modified RPE65 protein contains an additional G534SGATNFSLLKQAGDVEENPG554 polypeptide sequence at its C-terminus, while CreERT2 contains an additional Pro residue at its N-terminus. (B) Genotyping results from wild-type, Cre+/–, & Cre+/+ animals. (C) Western blot analysis of RPE65 expression. After normalization to the alpha -tubulin loading controls, the RPE65 expression levels in Cre+/– & Cre+/+ mice were estimated to be 59.7% & 1.1% of that for wild-type mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33784255), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")