Cre Antibodies
We offer Cre Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each Cre Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Cre Antibodies can be used in a variety of model species: Cre/lox System, Human, Mouse. Use the list below to choose the Cre Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Cre polyclonal antibodies and browse our Cre monoclonal antibody catalog.
Alternate Names for Cre Antibodies
anti-Cre antibody
5 Results for "cre" in Primary Antibodies
![Western Blot: Cre Antibody [NB100-56133] - Analysis of Cre using NB100-56133 at 1:2000. Lane 1: HeLa cells stably transfected with a CRE expression plasmid. Lane 2: HeLa cells transfected with a neo plasmid (negative control).](http://images.novusbio.com/fullsize/Cre-Antibody-Western-Blot-NB100-56133-img0004.jpg "Western Blot: Cre Antibody [NB100-56133] - Analysis of Cre using NB100-56133 at 1:2000. Lane 1: HeLa cells stably transfected with a CRE expression plasmid. Lane 2: HeLa cells transfected with a neo plasmid (negative control).")
![Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133] - Representative co-IF image showing YFP (green) and Cre (red) staining on a MG section from a Trp53L/L;R26Y female 3 days after Ad-K8-Cre injection. Trp53L/L;R26Y females (n=3); R26Y females (n=3). Scale bar, 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28194015/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cre-Antibody---BSA-Free-Immunohistochemistry-NB100-56133-img0008.jpg)
![Immunohistochemistry: Cre Antibody [NB100-56133] - FFPE tissue section from the cerebellum of a Cre transgenic mouse using NB100-56133 at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. The arrow denotes an example of a stained neuron. Cre staining is also seen in the cytosol of cerebellar Purkinje cells. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Cre-Antibody-Immunohistochemistry-NB100-56133-img0005.jpg)
![Immunohistochemistry: Cre Antibody [NB100-56133] - FFPE tissue section from the cerebellum of a Cre transgenic mouse using NB100-56133 at 1:2000. In this mouse line, Cre is expressed in the neurons brain. This mouse line also contains a LacZ reporter gene that becomes transcriptionally active upon Cre-mediated recombination. Brain tissue slices were stained with X-gal (blue color) prior to embedding. Cre staining is brown. Nuclear Red counterstain.](http://images.novusbio.com/fullsize/Cre-Antibody-Immunohistochemistry-NB100-56133-img0006.jpg)
![Immunohistochemistry: Cre Antibody [NB100-56133] - Enkephalin expression in the Paraventricular nucleus of the hypothalamus (PVN) in mouse brain. Antibody at 1:500. Image from verified customer review.](http://images.novusbio.com/fullsize/Cre-Antibody-Immunohistochemistry-NB100-56133-img0007.jpg)
![Western Blot: Cre Antibody - Unpurified [NB100-56134] - Lane 1: wild-type mouse (negative control). Lanes 2 and 3: brain from 2 types of Cre transgenic mice. Lane 4: recombinant Cre (positive control). Full-length Cre is detected at ~36-38 kDa. The lower molecular weight bands in lane 4 are breakdown products from the recombinant Cre protein.](http://images.novusbio.com/fullsize/Cre-Antibody-Western-Blot-NB100-56134-img0005.jpg "Western Blot: Cre Antibody - Unpurified [NB100-56134] - Lane 1: wild-type mouse (negative control). Lanes 2 and 3: brain from 2 types of Cre transgenic mice. Lane 4: recombinant Cre (positive control). Full-length Cre is detected at ~36-38 kDa. The lower molecular weight bands in lane 4 are breakdown products from the recombinant Cre protein.")
![Immunohistochemistry: Cre Antibody - Unpurified [NB100-56134] - Cre Antibody [NB100-56134] - Analysis of Cre in a FFPE tissue section from the hippocampus of a Cre transgenic mouse at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. Hematoxylin-eosin counterstain. A1 is a higher magnification of A.](http://images.novusbio.com/fullsize/Cre-Antibody-Immunohistochemistry-NB100-56134-img0004.jpg)
![Western Blot: Cre Antibody [NB100-56134] - Generation, identification, & Rpe65 expression analysis of Rpe65CreERT2 mice.(A) KI strategy to introduce a P2A-CreERT2 coding sequence in-frame with the final coding exon (exon 14) of the Rpe65 gene. Primer-binding sites & expected PCR product sizes are shown below the wild-type & targeted alleles. The neomycin cassette in the targeting vector was removed during expansion of the embryonic stem cell clone. FLP-recombinase was subsequently bred out by crossing with C57BL/6 mice. The KI allele allows cotranslational expression of RPE65-P2A & CreERT2 as 2 separate polypeptides. The modified RPE65 protein contains an additional G534SGATNFSLLKQAGDVEENPG554 polypeptide sequence at its C-terminus, while CreERT2 contains an additional Pro residue at its N-terminus. (B) Genotyping results from wild-type, Cre+/–, & Cre+/+ animals. (C) Western blot analysis of RPE65 expression. After normalization to the alpha -tubulin loading controls, the RPE65 expression levels in Cre+/– & Cre+/+ mice were estimated to be 59.7% & 1.1% of that for wild-type mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33784255), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56134_rabbit-polyclonal-cre-antibody-imgenex-img-5721-31020241555211.jpg)
![Immunohistochemistry-Frozen: Cre Antibody [NB100-56135] - Cochlea cross-section of Cre+/-mouse, stained for Cre (green) and counterstained for F-actin (red). Tissue was fixed in 4% paraformaldehyde and incubated overnight with the anti-Cre antibody 1:100 in PBS containing Triton X-100 0.1% and 10% fetal calf serum. Image from verified customer review.](http://images.novusbio.com/fullsize/Cre-Antibody-Immunohistochemistry-Frozen-NB100-56135-img0002.jpg "Immunohistochemistry-Frozen: Cre Antibody [NB100-56135] - Cochlea cross-section of Cre+/-mouse, stained for Cre (green) and counterstained for F-actin (red). Tissue was fixed in 4% paraformaldehyde and incubated overnight with the anti-Cre antibody 1:100 in PBS containing Triton X-100 0.1% and 10% fetal calf serum. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Cre Antibody [NB100-56135] - Analysis of tissue section from the hippocampus of a Cre transgenic mouse at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. Hematoxylin-eosin counterstain. Arrows point to negative glia.](http://images.novusbio.com/fullsize/Cre-Antibody-Immunohistochemistry-NB100-56135-img0001.jpg)
![Western Blot: Cre Antibody (PSH01-93) [NBP3-32219] - Western blot analysis of Cre on different lysates with Rabbit anti-Cre antibody (NBP3-32219) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T transfected with Cre recombinase cell lysate Lysates/proteins at 50 ng/Lane. Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32219) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32219_rabbit-cre-mab-psh01-93-27820241524267.jpg "Western Blot: Cre Antibody (PSH01-93) [NBP3-32219] - Western blot analysis of Cre on different lysates with Rabbit anti-Cre antibody (NBP3-32219) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T transfected with Cre recombinase cell lysate Lysates/proteins at 50 ng/Lane. Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32219) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cre Antibody (PSH01-93) [NBP3-32219] - Immunofluorescence analysis of paraffin-embedded 293T transfacted with or without Cre cells labeling Cre recombinase with Rabbit anti-Cre antibody (NBP3-32219) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32219, green) at 1/20,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32219_rabbit-cre-mab-psh01-93-29820241420910.jpg)
![Immunohistochemistry: Cre Antibody (PSH01-93) [NBP3-32219] - Immunohistochemical analysis of paraffin-embedded 293T transfacted with or without Cre cells with Rabbit anti-Cre antibody (NBP3-32219) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32219) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32219_rabbit-cre-mab-psh01-93-29820241420940.jpg)
![Immunocytochemistry/ Immunofluorescence: Cre Antibody (PSH01-93) [NBP3-32219] - Immunocytochemistry analysis of 293T transfacted with or without Cre cells labeling Cre recombinase with Rabbit anti-Cre antibody (NBP3-32219) at 1/20,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cre antibody (NBP3-32219) at 1/20,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32219_rabbit-cre-mab-psh01-93-29202410334021.jpg)
![Flow Cytometry: Cre Antibody (PSH01-93) [NBP3-32219] - Flow cytometric analysis of 293T transfacted with or without Cre cells labeling Cre recombinase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32219, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32219_rabbit-cre-mab-psh01-93-29202416372187.jpg)
![Western Blot: Cre Antibody (10212) [NBP2-42832] - Analysis of A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins 12.5 % SDS-PAGE Cre recombinase antibody [10212] dilution: 1:10000](http://images.novusbio.com/fullsize/Cre-Antibody-10212-Western-Blot-NBP2-42832-img0003.jpg "Western Blot: Cre Antibody (10212) [NBP2-42832] - Analysis of A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins 12.5 % SDS-PAGE Cre recombinase antibody [10212] dilution: 1:10000")
![Western Blot: Cre Antibody (10212) [NBP2-42832] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug whole cell lysate/extract of Cre-tagged protein expressing HeLa cells 7.5 % SDS-PAGE Cre recombinase antibody [10212] dilution: 1:5000](http://images.novusbio.com/fullsize/Cre-Antibody-10212-Western-Blot-NBP2-42832-img0002.jpg)
![Immunoprecipitation: Cre Antibody (10212) [NBP2-42832] - Analysis of 293T whole cell lysate/extract A : 20 ug whole cell lysate/extract of Cre recombinase protein expressing 293T cells B : Control with 2.5 ug of pre-immune mouse IgG C : Immunoprecipitation of Cre recombinase protein by 2.6 ug of Cre recombinase antibody 7.5% SDS-PAGE The immunoprecipitated Cre recombinase protein was detected by Cre recombinase antibody diluted at 1 : 5000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Cre-Antibody-10212-Immunoprecipitation-NBP2-42832-img0001.jpg)