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Rat Contactin-1 ELISA Kit (Chemiluminescence)

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ELISA: Rat Contactin-1 ELISA Kit (Chemiluminescence) [NBP2-75800] - Samples were spiked with high concentrations of Rat Contactin-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Contactin-1 ELISA Kit (Chemiluminescence) [NBP2-75800] - Samples were spiked with high concentrations of Rat Contactin-1 and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Rat Contactin-1 ELISA Kit (Chemiluminescence) [NBP2-75800] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

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Rat Contactin-1 ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Rat CNTN1 in samples. No significant cross-reactivity or interference between Rat CNTN1 and analogues was observed.
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 9.73%
Intra-Assay
CV% < 9.22%
Spike Recovery
89-114%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
CNTN1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Rat Contactin-1 ELISA Kit (Chemiluminescence)

  • CNTN1
  • contactin 1
  • Contactin1
  • Contactin-1
  • F11
  • F3
  • F3cam
  • Glycoprotein gp135
  • GP135
  • Neural cell surface protein F3

Background

Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.; SUBUNIT: Monomer. Interacts with CNTNAP1 in cis form. Binds to the carbonic-anhydrase like domain of protein-tyrosine phosphatase zeta. Interacts with NOTCH1 and TNR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  Rat Contactin-1 ELISA Kit (Chemiluminescence) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol CNTN1