ELISA: Human Collagen VI alpha 1 ELISA Kit (Colorimetric) [NBP2-75878] - Samples were spiked with high concentrations of Human Collagen VI alpha 1 and diluted with Reference Standard & Sample Diluent to produce samples ...read more
ELISA: Human Collagen VI alpha 1 ELISA Kit (Colorimetric) [NBP2-75878] - Standard Curve Reference
Storage of components varies. See protocol for specific instructions.
Kit Components
Components
Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent
Alternate Names for Human Collagen VI alpha 1 ELISA Kit (Colorimetric)
Collagen 6
collagen alpha-1(VI) chain
collagen VI, alpha-1 polypeptide
collagen, type VI, alpha 1
Collagen-6
human mRNA for collagen VI alpha-1 C-terminal globular domain10alpha 1 (VI) chain (61 AA)
OPLL
Background
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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