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![Western Blot: Collagen VI alpha 1 Antibody [NB120-6588] - Purification and imaging of collagen VI microfibrils from bovine cornea. Reducing SDS-PAGE (left hand panel) and western blot (right hand panel) of the central fraction of the void peak. Collagen VI chains were detected using a polyclonal rabbit anti collagen VI antibody. Arrows highlight bands at approximately 250 kDa, which corresponds to the alpha3 chain, and at 120 kDa which corresponds to alpha1 and alpha2 chains. Image collected and cropped by CiteAb from the following publication (www.linkinghub.elsevier.com/retrieve/pii/S1742706116306912) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-Western-Blot-NB120-6588-img0007.jpg "Western Blot: Collagen VI alpha 1 Antibody [NB120-6588] - Purification and imaging of collagen VI microfibrils from bovine cornea. Reducing SDS-PAGE (left hand panel) and western blot (right hand panel) of the central fraction of the void peak. Collagen VI chains were detected using a polyclonal rabbit anti collagen VI antibody. Arrows highlight bands at approximately 250 kDa, which corresponds to the alpha3 chain, and at 120 kDa which corresponds to alpha1 and alpha2 chains. Image collected and cropped by CiteAb from the following publication (www.linkinghub.elsevier.com/retrieve/pii/S1742706116306912) licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: Collagen VI alpha 1 Antibody [NB120-6588] - Feline blood vessels section. The image was captured with an epifluorescent microscope. Image submitted by a verified customer review](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-Frozen-NB120-6588-img0006.jpg)
![Flow Cytometry: Collagen VI alpha 1 Antibody [NB120-6588] - Effect of PTX3 on morphology, collagen production, & receptor expression. After 5 days incubation with 1 ug/ml PTX3, PBMCs were air-dried, fixed, & stained with anti-collagen-I or control antibodies. (Positive staining - red staining; nuclei - blue). Bar is 50 um. Following ICC staining, at least 100 elongated cells with oval nuclei were examined from at least 10 randomly selected fields, & the % of positive cells is expressed as mean +/- SEM (n = 3-5 donors). Supernatants from PBMC incubated in the absence (-) or presence (+) of 1 ug/ml PTX3 assessed by western blot. Adherent cells (macrophages & fibrocytes) were stained with collagen-VI (teal line SFM, red +PTX3) or control IgY (black SFM, orange +PTX3) antibodies. The data are representative of three separate experiments. Image collected & cropped by CiteAb from the following publication (www.dx.plos.org/10.1371/journal.pone.0119709) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-Flow-Cytometry-NB120-6588-img0008.jpg)
![Immunohistochemistry: Collagen VI alpha 1 Antibody [NB120-6588] - Showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval. Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-NB120-6588-img0005.jpg)
![Western Blot: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Western blot analysis of Collagen VI alpha 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Western-Blot-NBP2-67825-img0009.jpg "Western Blot: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Western blot analysis of Collagen VI alpha 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours.")
![Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunocytochemistry-Immunofluorescence-NBP2-67825-img0008.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Collagen VI alpha 1 with Rabbit anti-Collagen VI alpha 1 antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0014.jpg)
![Western Blot: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of Collagen VI on different lysates using anti-Collagen VI antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Human heart Lane 3: Human kidney](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Western-Blot-NBP2-67825-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunocytochemistry-Immunofluorescence-NBP2-67825-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunocytochemistry-Immunofluorescence-NBP2-67825-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunocytochemistry-Immunofluorescence-NBP2-67825-img0003.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human liver tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0004.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human lung tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0005.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded mouse colon tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0006.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-Collagen VI antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0010.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-Collagen VI alpha 1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0011.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Collagen VI antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0012.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Collagen VI with Rabbit anti-Collagen VI antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0013.jpg)
![Western Blot: Collagen VI alpha 1 Antibody [NBP2-15947] - Wild-type (WT) and COL6A1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-Western-Blot-NBP2-15947-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Collagen VI alpha 1 Antibody [NBP2-15947] - Wild-type (WT) and COL6A1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody [NBP2-15947] - Detects COL6A1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Collagen VI alpha 1 stained by diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15947-img0008.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP2-15947] - Mouse liver. COL6A1 antibody [C1C2], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Collagen-VI-Antibody-Immunohistochemistry-Paraffin-NBP2-15947-img0005.jpg)
![Western Blot: Collagen VI alpha 1 Antibody [NBP2-15947] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with COL6A1 antibody [C1C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Collagen-VI-Antibody-Western-Blot-NBP2-15947-img0007.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP2-15947] - Human hepatoma. COL6A1 antibody [C1C2], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Collagen-VI-Antibody-Immunohistochemistry-Paraffin-NBP2-15947-img0003.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human placenta shows moderate positivity in extracellular matrix.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP1-91193-img0010.jpg "Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human placenta shows moderate positivity in extracellular matrix.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Analysis in human placenta and pancreas tissues. Corresponding COL6A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP1-91193-img0006.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human testis shows weak positivity in extracellular matrix in peritubular myoid cells.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP1-91193-img0007.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human skin shows moderate positivity in extracellular matrix.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP1-91193-img0008.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91193] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP1-91193-img0009.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91195] - Collagen VI Antibody [NBP1-91195] - Staining in human gallbladder and pancreas tissues using anti-COL6A1 antibody. Corresponding COL6A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Collagen-VI-Antibody-Immunohistochemistry-Paraffin-NBP1-91195-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [NBP1-91195] - Collagen VI Antibody [NBP1-91195] - Staining in human gallbladder and pancreas tissues using anti-COL6A1 antibody. Corresponding COL6A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Collagen VI Antibody [NBP1-91195] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/Collagen-VI-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91195-img0005.jpg)
![Immunohistochemistry-Paraffin: Collagen VI Antibody [NBP1-91195] - Staining of human gallbladder shows high expression.](http://images.novusbio.com/fullsize/Collagen-VI-Antibody-Immunohistochemistry-Paraffin-NBP1-91195-img0006.jpg)
![Immunohistochemistry-Paraffin: Collagen VI Antibody [NBP1-91195] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Collagen-VI-Antibody-Immunohistochemistry-Paraffin-NBP1-91195-img0007.jpg)
![Western Blot: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Western blot analysis of extracts of various cell lines, using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-7J1P4-Western-Blot-NBP3-16869-img0003.jpg "Western Blot: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Western blot analysis of extracts of various cell lines, using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Immunohistochemistry of paraffin-embedded human liver using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-7J1P4-Immunohistochemistry-Paraffin-NBP3-16869-img0002.jpg)
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Immunohistochemistry of paraffin-embedded rat lung using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-7J1P4-Immunohistochemistry-Paraffin-NBP3-16869-img0001.jpg)
![Western Blot: Collagen VI Antibody [NBP1-59126] - Titration: 5% Milk, Positive Control: human LCL.](http://images.novusbio.com/fullsize/Collagen-VI-Antibody-Western-Blot-NBP1-59126-img0004.jpg "Western Blot: Collagen VI Antibody [NBP1-59126] - Titration: 5% Milk, Positive Control: human LCL.")
![Western Blot: Collagen VI Antibody [NBP1-59126] - Titration: 0.2-1 ug/ml, Positive Control: 721_B cell lysate.](http://images.novusbio.com/fullsize/Collagen-VI-Antibody-Western-Blot-NBP1-59126-img0003.jpg)
![ELISA: Human Collagen VI alpha 1 ELISA Kit (Colorimetric) [NBP2-75878] - Samples were spiked with high concentrations of Human Collagen VI alpha 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75878_human-collagen-vi-alpha-1-elisa-kit-colorimetric-132202416215364.jpg "ELISA: Human Collagen VI alpha 1 ELISA Kit (Colorimetric) [NBP2-75878] - Samples were spiked with high concentrations of Human Collagen VI alpha 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Collagen VI alpha 1 ELISA Kit (Colorimetric) [NBP2-75878] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75878_human-collagen-vi-alpha-1-elisa-kit-colorimetric-4320249133231.jpg)
![SDS-Page: Recombinant Human Collagen VI Protein [H00001291-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001291-Q01-1.jpg "SDS-Page: Recombinant Human Collagen VI Protein [H00001291-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Human Collagen VI ELISA Kit (Chemiluminescence) [NBP2-75875] - Samples were spiked with high concentrations of Human Collagen VI and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75875_human-collagen-vi-elisa-kit-chemiluminescence-1322024162613.jpg "ELISA: Human Collagen VI ELISA Kit (Chemiluminescence) [NBP2-75875] - Samples were spiked with high concentrations of Human Collagen VI and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Collagen VI ELISA Kit (Chemiluminescence) [NBP2-75875] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75875_human-collagen-vi-elisa-kit-chemiluminescence-4320248574365.jpg)