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Chymase/CMA1/Mast Cell Chymase Antibody

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Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with NBP2-27551 (0.5 ug/mL) in Lane A and probed with anti-DYKDDDDK Tag (1:3000) in lane C. Mock-transfected HEK293 probed with NBP2-27551 (1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with NBP2-27551 (0.5 ug/mL) in Lane A and probed with ...read more
Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - Staining of paraffin embedded Human Tonsil (Mast cells). Antibody at 1.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, ...read more
Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - Staining of human skin Epidermis shows detection on the apical side of the basal cell layer.
Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - Staining of paraffin embedded Human Testis (Mast cells). Antibody at 1.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Chymase/CMA1/Mast Cell Chymase Antibody Summary

Immunogen
Peptide with sequence C-QFRHPKYNTSTLHHD corresponding to internal region according to NP_001827.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CMA1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1.5 ug/mL
  • Immunohistochemistry-Paraffin 1.5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.5 - 1 ug/mL
Application Notes
WB: In transfected HEK293 transiently expressing full-length human CMA1 (myc and DYKDDDDK tagged), a band of approx. 35 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. IHC-P: Human skin shows staining on the apical side of the basal cell layer from the Epidermis.
Publications
Read Publication using NBP2-27551.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Chymase/CMA1/Mast Cell Chymase Antibody

  • Alpha-chymase
  • chymase 1 preproprotein transcript E
  • chymase 1 preproprotein transcript I
  • chymase 1, mast cell
  • Chymase
  • chymase, heart
  • chymase, mast cell
  • CMA1
  • CYH
  • CYM
  • EC 3.4.21
  • EC 3.4.21.39
  • Mast cell protease I
  • MCP3P
  • Mcpt5
  • MCT1
  • MGC119890
  • MGC119891

Background

Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CMA1
Uniprot