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CDC2L6 Antibody (CL14116)

Images

 
Staining of human skeletal muscle shows no positivity in myocytes as expected.
Staining of human prostate shows moderate nuclear positivity in glandular cells.
Western blot analysis in human cell line U-2197.
Orthogonal Strategies: Analysis in human testis and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.
Staining of human testis shows strong nuclear positivity in spermatids.
Staining of human cervix shows moderate nuclear positivity in squamous epithelial cells.
Staining in SH-SY5Y cell line showing specific staining of nuclear bodies and nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL14116
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
       

Orthogonal Strategies

 

Order Details

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CDC2L6 Antibody (CL14116) Summary

Immunogen
This antibody was developed against a Synthetic Peptide of Q9BWU1. Actual immunogen sequence is proprietary.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CDK19
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CDC2L6 Antibody (CL14116)

  • bA346C16.3
  • CDC2L6
  • CDC2-related protein kinase 6
  • CDK19
  • CDK8-like cyclin-dependent kinase
  • cell division cycle 2-like 6 (CDK8-like)
  • Cell division cycle 2-like protein kinase 6
  • Cell division protein kinase 19
  • cyclin-dependent kinase (CDC2-like) 11
  • Cyclin-dependent kinase 11
  • cyclin-dependent kinase 19
  • Death-preventing kinase
  • EC 2.7.11
  • EC 2.7.11.22
  • KIAA1028
  • KIAA1028CDK11

Background

CDC2L6 encodes a protein that is one of the components of the Mediator coactivator complex. The Mediator complex is a multiprotein complex required for transcriptional activation by DNA binding transcription factors of genes transcribed by RNA polymerase II. The protein encoded by this gene is similar to cyclin-dependent kinase 8 which can also be a component of the Mediator complex. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional CDC2L6 Products

Array NBP3-26850

Research Areas for CDC2L6 Antibody (NBP3-26850)

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Bioinformatics

Gene Symbol CDK19