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CDC2/CDK1 [p Thr161] Antibody

Images

 
Western Blot: CDC2/CDK1 [p Thr161] Antibody [NB100-81837] - Analysis of extracts from Hela cells untreated (lane 1) or treated with UV (lane 2) using CDC2 (Phospho-Thr161) antibody.
Immunohistochemistry-Paraffin: CDC2/CDK1 [p Thr161] Antibody [NB100-81837] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81837 (left) or the same antibody preincubated with blocking ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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CDC2/CDK1 [p Thr161] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human CDC2/CDK1 around the phosphorylation site of threonine 161 (T-Y-TP-H-E).
Modification
p Thr161
Specificity
Detects endogenous levels of CDC2/CDK1 only when phosphorylated at threonine 161.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:2000
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CDC2/CDK1 Overexpression Lysate
Publications
Read Publication using NB100-81837.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol, 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDC2/CDK1 [p Thr161] Antibody

  • CDC2
  • CDC28A
  • CDC2MGC111195
  • CDK1
  • cell cycle controller CDC2
  • Cell division control protein 2 homolog
  • Cell division protein kinase 1
  • cyclin-dependent kinase 1
  • DKFZp686L20222
  • EC 2.7.11.22
  • EC 2.7.11.23
  • G1 to S and G2 to M
  • p34 protein kinase
  • P34CDC2

Background

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDC2/CDK1 Antibody (NB100-81837)(1)

We have publications tested in 1 application: WB.


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WB
(1)
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Showing Publication 1 - 1 of 1.
Publication using NB100-81837 Applications Species
Li H. APC/C independent function of the spindle assembly checkpoint Thesis. 2017-01-01 (WB) WB

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDK1
Entrez
Uniprot