Western Blot: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis using CDK1 mouse mAb against Hela (1), Jurkat (2), K562 (3), A431 (4), MCF-7 (5), RAW264.7 (6), NIN/3T3 (7), PC-12 (8), and Cos7 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of Eca109 cells using CDK1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of spinal cord tissue using CDC2/CDK1 antibody (2G9). Image from verified customer review.
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of ovarian cancer tissues (left) and lung cancer tissues (right) using CDK1 mouse mAb with DAB staining.
34 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5 using NBP2-37626 in the following applications:
Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified
Alternate Names for CDC2/CDK1 Antibody (2G9) - BSA Free
CDC2
CDC28A
CDC2MGC111195
CDK1
cell cycle controller CDC2
Cell division control protein 2 homolog
Cell division protein kinase 1
cyclin-dependent kinase 1
DKFZp686L20222
EC 2.7.11.22
EC 2.7.11.23
G1 to S and G2 to M
p34 protein kinase
P34CDC2
Background
The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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