CD36 Antibody (FA6-152)

Originally discovered in platelets, cluster of differentiation 36, CD36, (also known as thrombospondin receptor, fatty acid translocase (FAT), platelet membrane glycoprotein IV (GPIV), and scavenger receptor class B, member 3 (SR-B3)) is a plasma membrane glycoprotein belonging to the class B scavenger receptor family (1,2). Human, mouse, and rat CD36 is synthesized as a 472 amino acid (a.a.) protein with a theoretical molecular weight of 53 kDa for the canonical isoform (3). Its domains include a short cytoplasmic tail at the N-terminal and C-terminal and a large extracellular loop flanked on each side by a transmembrane domain. The extracellular domain facilitates the update of fatty acids (FFAs), phospholipids, and cholesterol by forming two hydrophobic cavities, which was first modeled in the CD36 homologue, LIMP-2/ SCARB2 (4).

The expression of CD36 has been reported in platelets, erythrocytes, monocytes, differentiated adipocytes, skeletal muscle, mammary epithelial cells, spleen cells, some skin microdermal endothelial cells, and in cancer. Circulating levels of soluble CD36 (sCD36) has also been reported in chronic inflammatory disease such as type 2 diabetes and chronic kidney disease. CD36 participates in angiogenesis, innate immunity, and the clearance of apoptotic phagocytes. In lipid metabolism, CD36 functions as a macrophage receptor for oxidized LDL and as an adipocyte receptor/transporter for long-chain FFAs. Plasmodium falciparum, the parasite that causes malaria, binds CD36 via PfEMP1 proteins, tethering parasite-infected erythrocytes to endothelial receptors (5). Anti-CD36 isoantibodies have been detected in Type 1 CD36-deficient mothers and is implicated as the cause of fetal/neonatal alloimmune thrombocytopenia (6).

References

1) Febbraio, M., Hajjar, D. P., & Silverstein, R. L. (2001). CD36: a class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. The Journal of clinical investigation, 108(6), 785-791. PMID: 11560944

2) Silverstein RL, Febbraio M. (2000) CD36 and atherosclerosis. Curr Opin Lipidol. 2000 11(5):483-91. PMID: 11048891.

3) Endemann G, Stanton LW, Madden KS, Bryant CM, White RT, Protter AA. (1993) CD36 is a receptor for oxidized low density lipoprotein. J Biol Chem. 268(16):11811-6. PMID: 7685021.

4) Wang, J., & Li, Y. (2019). CD36 tango in cancer: signaling pathways and functions. Theranostics, 9(17), 4893-4908. PMID: 31410189

5) Hsieh FL, Turner L, Bolla JR, Robinson CV, Lavstsen T, Higgins MK. (2016) The structural basis for CD36 binding by the malaria parasite. Nat Commun. 7:12837. PMID: 27667267

6) Gruarin P, Ulliers D, Thorne RF, Alessio M. (2000) Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb. Mol Cell Biochem 214, 115-121. PMID: 11195795.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: IHC-Fr, IP, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, WB.

Showing Publications 1 - 10 of 12. Show All 12 Publications. Collapse Publications.

Publications using NB600-1423	Applications	Species
Li Y, Xu J, Chen W et al. Hepatocyte CD36 modulates UBQLN1-mediated proteasomal degradation of autophagic SNARE proteins contributing to septic liver injury Autophagy 2023-04-23 [PMID: 37014234]
Liu S, Zhang Y, Zheng X et al. Sulforaphane Inhibits Foam Cell Formation and Atherosclerosis via Mechanisms Involving the Modulation of Macrophage Cholesterol Transport and the Related Phenotype Nutrients 2023-04-28 (IHC-Fr, Mouse) Details: 1:100 dilution	IHC-Fr	Mouse
Liu S, Zhang Y, Zheng X et al. Sulforaphane Inhibits Foam Cell Formation and Atherosclerosis via Mechanisms Involving the Modulation of Macrophage Cholesterol Transport and the Related Phenotype Nutrients 2023-04-28 [PMID: 37432260] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence)	Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence
You M, Wu F, Gao M et al. Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis Redox Biology 2022-11-01 [PMID: 36252341] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Zeng H, Qin H, Liao M et al. CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing Molecular metabolism 2021-12-30 [PMID: 34974159] (WB, Mouse)	WB	Mouse
Yu H, Yang F, Zhong W et al. Secretory Galectin-3 promotes hepatic steatosis via regulation of the PPAR gamma/CD36 signaling pathway Cellular signalling 2021-08-01 [PMID: 33991615]
Luo X, Li Y, Yang P et al. Obesity induces preadipocyte CD36 expression promoting inflammation via the disruption of lysosomal calcium homeostasis and lysosome function EBioMedicine 2020-06-01 [PMID: 32516742] (IP, Mouse)	IP	Mouse
Li Y, Yang P, Zhao L et al. CD36 plays a negative role in the regulation of lipophagy in hepatocytes through an AMPK-dependent pathway. J. Lipid Res. 2019-01-20 [PMID: 30662007] (WB, Human)	WB	Human
Khalifeh-Soltani A, McKleroy W, Sakuma S et al. Mfge8 promotes obesity by mediating the uptake of dietary fats and serum fatty acids. Nat. Med. 2014-02-07 [PMID: 24441829] (WB, Mouse, Human)	WB	Mouse, Human
Hberg A, Skjell M, Michelsen AE et al. Soluble CD36 in Plasma Is Increased in Patients With Symptomatic Atherosclerotic Carotid Plaques Is Related to Plaque Instability. Stroke;39(11):3092-3095. 2008-01-01 [PMID: 18723424]
Janer J, ersson S, Haglund C et al. Placental Growth Factor Vascular Endothelial Growth Factor Receptor-2 in Human Lung Development. Pediatrics;122(2):340-346. 2008-01-01 [PMID: 18676552]
During A, Doraiswamy S, Harrison EH. Xanthophylls are preferentially taken up compared with {beta}-carotene by retinal cells via a SRBI-dependent mechanism. J Lipid Res;49(8):1715-1724. 2008-01-01 [PMID: 18424859]
Show All 12 Publications. Collapse Publications.

Array NB600-1423

• CD36 Antibodies
• CD36 ELISA Kits
• CD36 Lysates
• CD36 Proteins